Mango Malformation: I. Toxin Production Associated with Fusarium Pathogens

Wafaa Haggag M., Hazza M., Sehab A., Abd El-Wahab M.

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DOI: 10.4236/ajps.2011.22030                

Abstract

Eight Fusarium species i.e. F. subglutinans, F. solani, F. oxyspoum, F. sterilihyphosum, F. proliferatum, F. monili-forme, F. avena and F. chlamydspore isolated from mango malformed disease were tested for their ability to cause mango malformation disease and their production of moniliformin and total fumonisins (FB1 + FB2) using HPLC. A evaluated for moniliformin production, seven isolates were toxin producers, the production levels ranging from 0.51 to 8.90 µg/ml. The higher levels were produced by Fusarium subglutinans (8.51 µg/ml). Moderate concentrations of moniliformin was produced by F.moniliforme (6.90 µg/ml), F. oxysporum (6.30 µg/ml), F. proliferatum (4.10 μg/ml) and F. sterilihyphosum (1.10 μg/ml). Separation and identification of Fumonisin that was isolated from the pathogen- causing disease are made by (HPLC). A evaluated for total fumonisin production (FB1 + FB2), seven isolates were toxin producers, the production levels ranging from 0.10 to 8.30 µg/ml. The higher levels were produced by F. monili-forme (8.30 µg/ml. Moderate concentrations of fumonisin was produced by F .proliferatum (0.64 µg/ml) and F. subglutinans (0.50 µg/ml). Strong positive correlations between moniliformin and total fumonisins (FB1 + FB2) activities and malformation disease incidence by F. subglutinans, F. solani, F. oxyspoum, F. sterilihyphosum, F. proliferatum was observed.

Keywords

Fusarium, Mango Malformation, Moniliformin And Fumonisins

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Chulze, S. N., Ramirez, M. L., Farnochi, M. C., Pascale, M., Visconti, A. & March, G. (1996). Fusarium and fumonisins occurrence in Argentinian corn at different ear maturity stages. Journal of Agricultural and Food Chemistry 44, 2797-2801
[2]	Haggag, Wafaa and Abd Wahab (2009). First Report of Fusarium sterilihyphosum and F. proliferatum-Induced Malformation Disease of Mango in Egypt. J. of Plant Pathology 91(1), 231-240 (232).
[3]	Khaskheli, M. I.; Pathan, M.; Jiskani, M.; Soomro, M. H. and Poussio, G. B. (2008). First record of Fusarium Nivale (FR.) ces. Associated with mango malformation disease (MMD) in Pakistan. Pak. J. Bot. 40(6): 2641-2644.
[4]	Kumar, J. and Beniwal, S. P. S. (1987). A method of estimating cultivar susceptibility against mango malformation. Trop. Pest Management 33:208-210.
[5]	Kumar, J. and Beniwal, S. P. S (1992). Mango malformation, In: Plant Diseases of international Importance. Kumar, J., Chaube, H.S., Singh ,U. S., Mukhopadhyay, A. N. (ed) 3:357-393.New York: Prentice Hall. 456p.
[6]	Miller, J. D. (1994). Epidemology of Fusarium ear diseases. pp. 19-36 in J. D. Miller and H. L. Trenholm, eds. mycotoxins in grain . Eagan press, St. Paul, MN.
[7]	Miller, J. D.; Savard, M. E.; Sibilia, A.; Rapior, S.; Hocking, A. D. and Pitt, J. I. (1993). Production of fumonisins and fusarins by Fusarium moniliforme from Southeast Asia. Mycologia 85:385-391.
[8]	Nelson, P. E.; Plattner, R. D.; Shackelford, D. D. and Desjardins, A. E. (1992 ). Fumonisin B1 production by Fusarium species other than F. moniliforme in section Liseola and by some related species. Appl . Environ Microbiol. 58(3): 984-989.
[9]	Ploetz, R. C. (2001). Malformation: a unique and important disease of mango, Mangifera indica L. In: Summerell, B.A., Leslie, J. F., Backhouse, D. and Bryden, W. L. (eds) Fusarium: Paul E. Nelson Memorial Symposium. American Phytopathological Society (APS) Press, St Paul, Minnesota, pp. 233-247.
[10]	Ram, S. (1991). Horticultural aspects of mango malformation. Acta Horticulture 291:235-252.
[11]	Savard, M. E. and Miller, J. D. (1992). Characterization of fusarin, A new fusarin from Fusarium moniliforme. J. Nat. Prod. 55:64-70.
[12]	Scott, P.M. and Lwrence, G. A. (1987). Liquid chromatographic determination and stability of the Fusarium mycotoxin moniliformin in cereal grains. J. Assoc. of. Anal. Chem. 70:850-853.
[13]	Singh, Z. and Dhillon, B. S. (1986). Effect of naphthalene acetic acid, ethrel, dikegulac and hand deblossoming on floral malformation , flowering, yield and fruit quality of mango (mangifera indica L.). J. Phytopath.120:245-248.
[14]	Singh, Z. and Dhillon, B. S.(1989a). Presence of Malformin –like substance in malformed floral tissues of mango. J. Phytopathol.125:117-123.
[15]	Singh, Z. and Dhillon, B.S. (1989b). Hormonal changes associated with vegetative malformation of mango (Mangifera indica L.). J. of Phytopathology 125, 193-197.
[16]	Singh, Z. and Dhillon, B. S. (1990). Comparative developmental morphology of normal and malformed floral organs of mango (Mangifera indicaL.). Trop. Agric. (Trinidad). 67:143-148.