Leaf Traits and Histochemistry of Trichomes of Conocarpus lancifolius a Combretaceae in Semi-Arid Conditions
Amina Redha, Naemah Al-Mansour, Patrice Suleman, Mohamad Afzal, Redha Al-Hasan
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DOI: 10.4236/ajps.2011.22018   PDF    HTML     7,986 Downloads   15,322 Views   Citations

Abstract

Leaf traits, structure and water status of Conocarpus lancifolius, a Combretaceae were investigated under semi-arid conditions. The leaf traits examined included leaf area and thickness, stomatal distribution, sclerophylly, succulence and relative water content. Additionally, the types of secretory structures, histochemistry of trichomes, and chemical nature of the cuticlular waxes were evaluated. Leaves showed xerophytic characteristics including a high degree of sclerophylly, thick cuticle and outer epidermal cell wall, low relative water content and high trichome density on younger leaves. The species has two types of trichomes; a secretory, short-stalked capitate trichome and a non-secretory trichome with a bulbous base and a pointed tip. The leaves also have a pair of extrafloral nectaries on both sides of the distal end of the petiole, 3-4 pairs near the leaf apex and two secretory ducts or cavities on mature leaves that secreted polysaccharides, epicuticlar waxes and polyphenols. Compared to young leaves mature leaves had almost 3 times total cuticular wax deposit or load. The most abundant fatty acids were palmitic, stearic, nondecanoic, behenic and arachidic acids. The leaf traits and structures are discussed in relation to semi-arid habitat.

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A. Redha, N. Al-Mansour, P. Suleman, M. Afzal and R. Al-Hasan, "Leaf Traits and Histochemistry of Trichomes of Conocarpus lancifolius a Combretaceae in Semi-Arid Conditions," American Journal of Plant Sciences, Vol. 2 No. 2, 2011, pp. 165-174. doi: 10.4236/ajps.2011.22018.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] A. A. Agrawal and D. A. Spiller, “Polymorphic buttonwood: effects of disturbance on resistance to herbivores in green and silver morphs of a Bahamian shrub,” American Journal of Botany, Vol. 91, 2004, pp. 1990-1997.
[2] L. Ascensao and M. S. S. Pais, “Glandula,r trichomes of Artemisa campestris (ssp. Maritima): ontogeny and histochemistry of the secretory product,” Botanical Gazette, Vol. 148, 1987, pp. 221-227.
[3] N. C. W. Beadle, “Soil phosphate and its role in molding segments of Australian flora and vegetation with reference to xeromorphy and sclerophylly”. Ecology, Vol. 47, 1966, pp. 992-1007.
[4] T. M. Bourett, R. J. Howard, D. P. O’keefe and D. L. Hallahan, “Gland development on leaf surfaces of Nepeta racemosa,” International Journal of Plant Science, Vol. 155, 1994, pp. 623-632.
[5] M. C. Brundrett, B. Kendrick and C. A. Peterson, “Efficient lipid staining in plant material with Sudan 7B or fluorol yellow 088 in polyethylene glycol-glycerol,” Biotechnic. and Histochemistry, Vol. 66, 1991, pp. 111-116.
[6] F. Bussoti, D. Bettini, P. Grossoni, R. Mansuino, C. Soda and C. Tani, “Structure and functional traits of Quercus ilex in response to water availability,” Environmental Experimental Botany, Vol. 47, 2002, pp. 11-23.
[7] K. Chartzoulakis, A. Bosabalidis, A. Patakas and A. Vemmos, “Effect of water stress on water relation, gas exchange and leaf structure of olive tree,” Acta Horticulturae, Vol. 537, 2000, pp. 241-247.
[8] C. Edwards, J. Read and G. Sanson, “Characterizing sclerophyll: some mechanical properties of leaves from heath and forest,” Oecologia, Vol. 123, 2000, pp. 158-167.
[9] T. S. Elias, 1983. “Extrafloral nectaries: their structure and distribution,” In: B, Bentley and T. Elias, Eds., The Biology of Nectaries. New York, NY, Columbia University Press, 1983, pp. 174-203.
[10] K. Esau, “Anatomy of Seed Plants.John,” Wiley and Sons NewYork, 1997.
[11] A. Fahn, 1988. “Secretory tissues in vascular plants,” New Phytologist, Vol. 108, pp. 229-257.
[12] P. B. Gahan, “Plant histochemistry and cytochemistry,” London, Academic Press, 1984.
[13] E. Garnier and G. Laurent, “Leaf anatomy, specific mass and water content in congeneric annual and perennial grass species,” New Phytologist Vol. 128, 1994, pp. 725-736.
[14] L. Gonzàlez, and M. Gonzàalez-Vilar, “Determination of relative water content.” In: M. J. R. Roger, Ed., Handbook of Plant Ecophysiology Techniques. Kluwer Acad. Pub. Dordrecht, ND, 2001, pp. 207-212.
[15] Y. Heslop-Harrison and J. Heslop-Harrison, “The digestive glands of Pinguicula: structure and cytochemistry,” Annals of Botany, Vol. 47, 1981, pp. 293-319.
[16] M. G. Holmes and D. R. Keiller, “Effects of pubescence and waxes in the reflectance of leaves in the ultraviolet and photosynthetic wavebands: a composition of a range of species,” Plant Cell and Environment Vol. 25, 2002, pp. 85-95.
[17] W. A. Jensen, “Botanical Histochemistry: Principles and Practice,” Freeman, San Francisco, CA., 1962.
[18] G. Jordan, R. A. Dillon and P. H. Weston, “Solar radiation as a factor in the evolution of scleromorphic leaf anatomy in Proteaceae,” American Journal of Botany Vol. 92, 2005, pp. 789-189.
[19] G. Karabourniotis and J. Bornman, “Penetration of UV-A, UV-B and blue light through the leaf trichome layers of two xeromorphic plants, Olive and Oak, measured by optical fibre microprobes,” Physiologia Plantarum Vol. 105, 1999, pp. 655-661.
[20] K. Kathiresan and B. L. Bingham, “Biology of mangrove and mangrove ecosystems. Advance Marine Biology,” Vol. 40, 2001, pp. 81-251.
[21] K. Kock, C. Neinhius, H. J. Ensikat and W. Barthlott, “Self assembly of epiculticular waxes on living plant surfaces imaged by atomic force microscopy (AFM),” Journal of Experimental Botany, Vol. 55, 2004, pp. 711-718.
[22] S. Koptur, “Plants with extrafloral nectatries and ants in Everglades habitats,” Florida Entomologist, Vol. 75, 1992, pp. 39-50.
[23] A. Manal, A. Redha and P. Suleman, “Polyamine accumulation and osmotic adjustment as adaptive response to water and salinity stress in Conocarpus lanciflolius,” Functional Plant Science and Biotechnology, Vol. 3, 2009, pp. 42-48.
[24] S. Misra, A. K. Datta, S. M. Chattopadhyay, A. Choudhury and A. Ghosh, “Hydrcarbons and wax esters from seven species of mangrove leaves,” Phytochem. Vol. 26, 1987, pp. 3265-3268.
[25] C. O. Mosjidis, C. M. Peterson and J. A. Mosjidis, “Developmental differences in the location of polyphernols and condensed tannins in the leaves and stems of Sericea lespedeza, Lespedeza cuneata,” Annals of Botany Vol. 65, 1990, pp. 355-360.
[26] C. Neinhuis, K. Koch and W. Barthlott, “Movement and regeneration of epicuticular waxes through plant cuticles,” Planta Vol. 213, 2001, pp. 427-434.
[27] J. J. Oertli, S. H. Lips and M. Agami, “The strength of sclerophyllous cells to resist collapse due to negative turgor pressure,” Acta Oecologia Vol. 11, 1990, pp. 281-289.
[28] A. G. Richardson and G. Berlyn, “Changes in foliar spectral reflectance and chlorophyll fluorescence of four temperate species following branch cutting,” Tree Physiology Vol. 43, 2002, pp. 499-506.
[29] T. W. Schoener, “Leaf damage in island buttonwood, Conocarpus erectus: correlations with pubescence, island area, isolation and distribution of major carnivores,” Oikos Vol. 53, 1988, pp. 253-266.
[30] G. T. Wagner, “Secreting glandular trichomes: More than just hairs,” Plant Physiology, Vol. 96, 1991, pp. 675-679.
[31] T. J. Walton, “Waxes, cutin and suberin”. In: J. L. Harwood and J. Boyer, Eds., Methods in Plant Biochemistry, Vol. 4, 1990, pp. 106-158.
[32] E. Werker, E. Putievsky, U. Ravid, N. Dudai and I. Katzir, “Glandular hairs and essential oil in developing leaves of Ocimmum basilicum L. (Lamiaceae),” Annals of Botany, Vol. 71, 1993, pp. 43-50.
[33] J. Y. Zhang, C. D. Broeckling, E. B. Bancafllor, M. K. Sledge, L. W. Sumner and Z. Y. Want, “Overexpression of WXP1, a putative Medicago truncatula AP2 domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa),” Plant J. Vol. 42, 2005, pp. 689-707.

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