Genetic Diversity of Great Dane Breed Using Ten Microsatellites: Impact of Breeding Control over the Breeding Line
Gino Noris, Carla Santana, Mariana Herrán-Aguirre, Marco Antonio Meraz-Ríos, Mario Pérez-Martínez, Carlos Esquivel-Lacroix, Leonor C. Acosta-Saavedra, Eduardo Rodríguez, María de la Paz Juaréz, Emma S. Calderón-Aranda, Rocío Gómez
BIMODI (Biología Molecular Diagnóstica), Querétaro, Qro., México.
Departamento de Biomedicina Molecular, Cinvestav-IPN, México D.F., México.
Departamento de Morfología, Facultad de Medicina Veteri-naria y Zootecnia, Universidad Nacional Autónoma de México, México D.F., México.
Departamento de Reproducción, Facultad de Medicina Vete-rinaria y Zootecnia, Universidad Nacional Autónoma de México, México D.F., México.
Departamento de Toxicología, Cinvestav-IPN. Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, México D.F., México.
DOI: 10.4236/ojgen.2014.42011   PDF    HTML     5,728 Downloads   9,092 Views   Citations

Abstract

The American Kennel Club and the Fédération Cynologique Internationale recognize two phenotypic variants of Great Dane breed denominated American and European varieties. Historically, these varieties have been segregated according to morphological characteristics. In an attempt to obtain a better characterization, breeders have been interested in the genetic parameters that could evaluate the within-breed diversity. In this document, we studied the genetic structure of Great Dane breed with 10 STR markers in 88 dogs using capillary electrophoresis. Cluster analysis, population differentiation and phylogenetic analyses revealed that American and European varieties are genetically independent. Nevertheless, within the American variety a genetic stratification was found. Additionally, a high misclassification (28%) was detected, which could be due to wrong registration or false paternity. Our results support the importance to deem genetic markers as useful tools in breeding control. Similarly, these studies serve as an accurate reference to establish standards by dog breeding associations and for choosing among dogs for inter-breeding. Nevertheless, genetic tools are only a complement of morphological methods, since both are reshuffling the control over the breeding line. Notwithstanding, this database provides an overall and scape concerning the impact of genetic diversity within-breed. Unmistakably, more databases are needed to increase the quality of the breeding line as well as the number of STR in order to study, with more detail, the genetic structure in the Great Dane race.

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Noris, G. , Santana, C. , Herrán-Aguirre, M. , Meraz-Ríos, M. , Pérez-Martínez, M. , Esquivel-Lacroix, C. , Acosta-Saavedra, L. , Rodríguez, E. , Paz Juaréz, M. , Calderón-Aranda, E. and Gómez, R. (2014) Genetic Diversity of Great Dane Breed Using Ten Microsatellites: Impact of Breeding Control over the Breeding Line. Open Journal of Genetics, 4, 78-86. doi: 10.4236/ojgen.2014.42011.

Conflicts of Interest

The authors declare no conflicts of interest.

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