Protective Effects of a Novel Preparation Consists of Concentrated Dead Sea Water and Natural Plants Extracts against Skin Photo-Damage

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DOI: 10.4236/jcdsa.2014.41002    2,640 Downloads   4,232 Views   Citations


Background: Exposure to solar UV rays has deleterious effects on skin appearance through physiological and structural alterations that eventually lead to skin photo-damage. Aims: To test the photo-protective effect of a novel preparation, Dead Sea Osmoter Concentrate (DSOC), containing high concentration of Dead Sea water. Methods: Human skin organ culture was used as a model to assess the biological effects of UVB irradiation and the protective effect of topical application of DSOC preparation. Skin pieces were analyzed for mitochondrial activity by Alamar blue (resazurin) assay, for apoptosis by caspase 3 assay, for detoxification by proteasome 20S sub-unit activity and for skin hydration by aquaporin 3 (AQP3) membrane channels expression. Human subjects were tested to evaluate the effect of DSOC Serum, a topical formula for facial skin, on skin hydration by a corneometer. Results: UVB irradiation induced cell apoptosis in the epidermis of skin organ cultures and decreased their proteasome activity and AQP3 expression. Topical application of DSOC significantly attenuated all these effects. In human subjects, an elevation in the intense skin moisture, which was higher relatively to subjects’ regular moisturizer, was observed. Moreover, DSOC Serum boosted the hydration effect together with regular moisturizer. Conclusions: The results clearly demonstrate that DSOC preparation can significantly improve the skin capability in coping with UVB rays stress in different cellular functions such as anti-apoptotic properties as well as detoxification and hydration recovery that can attenuate biological effects of skin photo-damage. Topical application of DSOC Serum had contributed to skin appearance by a strong hydration impact, also as a booster.

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M. Portugal-Cohen, I. Afriat-Staloff, Y. Soroka, M. Frusic-Zlotkin, G. Schlippe, W. Voss and Z. Ma’or, "Protective Effects of a Novel Preparation Consists of Concentrated Dead Sea Water and Natural Plants Extracts against Skin Photo-Damage," Journal of Cosmetics, Dermatological Sciences and Applications, Vol. 4 No. 1, 2014, pp. 7-15. doi: 10.4236/jcdsa.2014.41002.


[1] Y. Miyachi and O. Ishikawa, “Dermal Connective Tissue Metabolism in Photoageing,” Australasian Journal of Dermatology, Vol. 39, No. 1, 1998, pp. 19-23. tb01235.x
[2] S. Pillai, C. Oresajo and J. Hayward, “Ultraviolet Radiation and Skin Aging: Roles of Reactive Oxygen Species, Inflammation and Protease Activation, and Strategies for Prevention of Inflammation-Induced Matrix Degradation —A Review,” International Journal of Cosmetic Science, Vol. 27, No. 1, 2005, pp. 17-34.
[3] T. M. Runger, “Role of UVA in the Pathogenesis of Melanoma and Non-Melanoma Skin Cancer. A Short Review,” Photodermatology, Photoimmunology & Photomedicine, Vol. 15, No. 6, 1999, pp. 212-216.
[4] M. Ichihashi, M. Ueda, A. Budiyanto, T. Bito, M. Oka, M. Fukunaga, K. Tsuru and T. Horikawa, “UV-Induced Skin Damage,” Toxicology, Vol. 189, No. 1-2, 2003, pp. 21-39. S0300-483X(03)00150-1
[5] R. Kohen and A. Nyska, “Oxidation of Biological Systems: Oxidative Stress Phenomena, Antioxidants, Redox Reactions, and Methods for Their Quantification,” Toxicologic Pathology, Vol. 30, No. 6, 2002, pp. 620-650.
[6] S. Verdier-Sevrain and F. Bonte, “Skin Hydration: A Review on Its Molecular Mechanisms,” Journal of Cosmetic Dermatology, Vol. 6, No. 2, 2007, pp. 75-82. 00300.x
[7] A. Watkinson, C. Harding, A. Moore and P. Coan, “Water Modulation of Stratum Corneum Chymotryptic Enzyme Activity and Desquamation,” Archives of Dermatological Research, Vol. 293, No. 9, 2001, pp. 470-476.
[8] S. J. Shan, T. Xiao, J. Chen, S. L. Geng, C. P. Li, X. Xu, Y. Hong, C. Ji, Y. Guo, H. Wei, W. Liu, D. Li and H. D. Chen, “Kanglaite Attenuates UVB-Induced Down-Regulation of Aquaporin-3 in Cultured Human Skin Keratinocytes,” International Journal of Molecular Medicine, Vol. 29, 2011, pp. 625-629.
[9] S. Halevy and S. Sukenik, “Different Modalities of Spa Therapy for Skin Diseases at the Dead Sea Area,” Archives of Dermatology, Vol. 134, No. 11, 1998, pp. 14161420. archderm.134.11.1416
[10] S. W. Moses, M. David, E. Goldhammer, A. Tal and S. Sukenik, “The Dead Sea, a Unique Natural Health Resort,” Israel Medical Association Journal, Vol. 8, 2006, pp. 483-488.
[11] Y. Soroka, Z. Ma’or, Y. Leshem, L. Verochovsky, R. Neuman, F. M. Bregegere and Y. Milner, “Aged Keratinocyte Phenotyping: Morphology, Biochemical Markers and Effects of Dead Sea Minerals,” Experimental Gerontology, Vol. 43, No. 10, 2008, pp. 947-957. 2008.08.003
[12] Z. Ma’or, S. Yehuda and W. Voss, “Skin Smoothing Effects of Dead Sea Minerals: Comparative Profilometric Evaluation of Skin Surface,” International Journal of Cosmetic Science, Vol. 19, No. 3, 1997, pp. 105-110.
[13] X. Wang, F. Bregegere, Y. Soroka, A. Kayat, G. Redziniak and Y. Milner, “Enhancement of Fas-Mediated Apoptosis in Ageing Human Keratinocytes,” Mechanisms of Ageing and Development, Vol. 125, No. 3, 2004, pp. 237-249.
[14] Y. Matsumura and H. N. Ananthaswamy, “Toxic Effects of Ultraviolet Radiation on the Skin,” Toxicology and Applied Pharmacology, Vol. 195, No. 3, 2004, pp. 298-308. j.taap.2003.08.019
[15] M. Iwata, T. Takebayashi, H. Ohta, R. E. Alcalde, Y. Itano and T. Matsumura, “Zinc Accumulation and Metallothionein Gene Expression in the Proliferating Epidermis during Wound Healing in Mouse Skin,” Histochemistry and Cell Biology, Vol. 112, No. 4, 1999, pp. 283-290. 80050449
[16] Z. Y. Wang, R. Agarwal, D. R. Bickers and H. Mukhtar, “Protection against Ultraviolet B Radiation-Induced Photocarcinogenesis in Hairless Mice by Green Tea Polyphenols,” Carcinogenesis, Vol. 12, No. 8, 1991, pp. 1527-1530.
[17] D. Bagchi, M. Bagchi, S. J. Stohs, D. K. Das, S. D. Ray, C. A. Kuszynski, S. S. Joshi and H. G. Pruess, “Free Radicals and Grape Seed Proanthocyanidin Extract: Importance in Human Health and Disease Prevention,” Toxicology, Vol. 148, No. 2-3, 2000, pp. 187-197. (00)00210-9
[18] S. K. Mantena and S. K. Katiyar, “Grape Seed Proanthocyanidins Inhibit UV-Radiation-Induced Oxidative Stress and Activation of MAPK and NF-KappaB Signaling in Human Epidermal Keratinocytes,” Free Radical Biology and Medicine, Vol. 40, No. 9, 2006, pp. 1603-1614.
[19] M. A. Carluccio, L. Siculella, M. A. Ancora, M. Massaro, E. Scoditti, C. Storelli, F. Visioli, A. Distante and R. De Caterina, “Olive Oil and Red Wine Antioxidant Polyphenols Inhibit Endothelial Activation: Antiatherogenic Properties of Mediterranean Diet Phytochemicals,” Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 23, 2003, pp. 622-629. 32.A0
[20] Y. Kimura and M. Sumiyoshi, “Olive Leaf Extract and Its Main Component Oleuropein Prevent Chronic Ultraviolet B Radiation-Induced Skin Damage and Carcinogenesis in Hairless Mice,” Journal of Nutrition, Vol. 139, No. 11, 2009, pp. 2079-2086.
[21] M. Portugal-Cohen, Y. Soroka, M. Frusic-Zlotkin, L. Verkhovsky, F. M. Bregegere, R. Neuman, R. Kohen and Y. Milner, “Skin Organ Culture as a Model to Study Oxidative Stress, Inflammation and Structural Alterations Associated with UVB-Induced Photodamage,” Experimental Dermatology, Vol. 20, No. 9, 2011, pp. 749-755.
[22] E. Filipowicz, P. Adegboyega, R. L. Sanchez and Z. Gatalica, “Expression of CD95 (Fas) in Sun-Exposed Human Skin and Cutaneous Carcinomas,” Cancer, Vol. 94, No. 3, 2002, pp. 814-819.
[23] K. Kannan and S. K. Jain, “Oxidative Stress and Apoptosis,” Pathophysiology, Vol. 7, No. 3, 2000, pp. 153-163.
[24] A. L. Bulteau, M. Moreau, C. Nizard and B. Friguet, “Proteasome and Photoaging: The Effects of UV Irradiation,” Annals of the New York Academy of Sciences, Vol. 1100, 2007, pp. 280-290.
[25] R. Koziel, R. Greussing, A. B. Maier, L. Declercq and P. Jansen-Durr, “Functional Interplay between Mitochondrial and Proteasome Activity in Skin Aging,” Journal of Investigative Dermatology, Vol. 131, 2011, pp. 594-603.
[26] N. Ikarashi, T. Ushiki, T. Mochizuki, T. Toda, T. Kudo, K. Baba, M. Ishii, K. Ito, W. Ochiai and K. Sugiyama, “Effects of Magnesium Sulphate Administration on Aquaporin 3 in Rat Gastrointestinal Tract,” Biological and Pharmaceutical Bulletin, Vol. 34, No. 2, 2011, pp. 238-242.
[27] C. Cao, S. Wan, Q. Jiang, A. Amaral, S. Lu, G. Hu, Z. Bi, N. Kouttab, W. Chu and Y. Wan, “All-Trans Retinoic Acid Attenuates Ultraviolet Radiation-Induced Down-Regulation of Aquaporin-3 and Water Permeability in Human Keratinocytes,” Journal of Cellular Physiology, Vol. 215, No. 2, 2008, pp. 506-516.
[28] S. Kang, J. H. Chung, J. H. Lee, G. J. Fisher, Y. S. Wan, E. A. Duell and J. J. Voorhees, “Topical N-Acetyl Cysteine and Genistein Prevent Ultraviolet-Light-Induced Signaling that Leads to Photoaging in Human Skin in Vivo,” Journal of Investigative Dermatology, Vol. 120, 2003, pp. 835-841.
[29] E. Wineman, M. Portugal-Cohen, Y. Soroka, D. Cohen, G. Schlippe, W. Voss, S. Brenner, Y. Milner, N. Hai and Z. Ma’or, “Photo-Damage Protective Effect of Two Facial Products, Containing a Unique Complex of Dead Sea Minerals and Himalayan Actives,” Journal of Cosmetic Dermatology, Vol. 11, No. 3, 2012, pp. 183-192.

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