Prohibitins, novel vitamin K2 target factors in osteoblast

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Vitamin K2 (VK2, menaquinone) is a drug for osteoporosis. VK2 acts as a cofactor for γ-glutamyl carboxylase, which catalyzes the carboxylation of specific glutamic acid residues (γ-carboxylation) of substrate proteins. Here we demonstrate that VK2 also regulate osteoblastgenic marker gene expression. Using VK2-immobilzed nanobeads new target proteins were purified and identified from osteoblastic cell line. They are prohibitin 1 and 2 (PHB1 & 2), respectively. To confirm the PHBs function on VK2-dependent transcription, PHB1 & 2 were knock-down and osteocalcin gene 2 transcriptions were analyzed, indicating that PHBs regulate VK2-dependent transcription. Taken together PHBs are VK2 target proteins for osteoblastgenic transcription.

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Uebi, T. , Umeda, M. , Maekawa, N. , Karasawa, S. , Handa, H. and Imai, T. (2013) Prohibitins, novel vitamin K2 target factors in osteoblast. Journal of Biosciences and Medicines, 1, 1-4. doi: 10.4236/jbm.2013.13001.


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