Genetic expression of Col-2A and Col-10A as a function of administration of IGF-1 & TGF-β with and without anterior mandibular repositioning appliance on the growth of mandibular condylar cartilage in young rabbit

A. S. Patil, R. B. Sable, R. M. Kothari, P. Nagarajan

Deartment of Orthodontics and Dentofacial Orthopedics, Bharati Vidyapeeth Dental College and Hospital, Bharati Vidyapeeth Deemed University, Pune, India.
Rajiv Gandhi Institute of Biotechnology, Bharati Vidyapeeth Deemed University, Pune, India.

DOI: 10.4236/ojst.2013.39A002                

Abstract

New Zealand (NZ) young rabbits with the administration of insulin-like growth factor (IGF-1) and transforming growth factor-β (TGF-β) with and without mandibular anterior repositioning appliances are explored for the growth of the mandibular condylar cartilage (MCC). 32 growing NZ and rabbits were divided into 4 groups: the group with saline injection in TMJ, the group which received growth factor injection in TMJ, the group which received anterior positioning appliance and the group which received growth factors injection as well as mandibular repositioning appliance. Gene expression was studied by real-time RT-PCR and cartilage growth by histomorphometry. Administration of growth factors along with mandibular repositioning appliances has induced 1) 1.70-fold expression of Col-2Agene (p value < 0.0005) and 2) 1.47-fold expression of Col-10Agene (p value < 0.0005). In contrast, administration of only mandibular repositioning appliances induced 1) 1.28-fold expression of Col-2Agene (p value < 0.0005) and 2) merely 0.62-fold expression of Col-10Agene (p value < 0.0005), while administration of growth factors only induced 1) mere 0.56-fold expression of Col-2Agene (p value <0.0005) and 2) 0.86-fold expression of Col-10A gene (p value < 0.0005). Administration of growth factors along with mandibular repositioning appliances causes an increase in genetic expressions which have been corroborated by histomorphometry and validated by statistical analysis, during an accelerated growth of mandibular condylar cartilage. Administration of growth factors in the TMJ could provide a synergistic role along with mandibular repositioning appliances for treatment of mandibular retrognathism as well as disorders on the MCC. 

Keywords

Transforming Growth Factor-β (TGF-β); Insulin-Like Growth Factor (IGF-1); Condylar Cartilage Growth; Mandibular Repositioning Appliances; Col-2A; Col-10A

Conflicts of Interest

The authors declare no conflicts of interest.

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