Expression of Nucleoprotein Gene of CTN Strain Rabies Virus from China in E. coli with Antigenicity

Wangbin Cao, Ying He, Qiumei Shi, Cairan Yang, Yanying Zhang

Department of Animal Science, Hebei Key Laboratory of Preventive Veterinary, Hebei Normal University of Science & Technology, Qinhuangdao, China.

DOI: 10.4236/ojvm.2013.37050                

Abstract

The nucleoprotein (NP) gene of rabies CTN strain isolated from China was recombined into pMal-c2x. The antigenicity of the recombined MBP-NP fusion proteins was examined by western blotting and by enzyme linked immunosorbent assay (ELISA). The results demonstrated that the recombined protein possesses predominant antigenicity.

Keywords

Rabies Virus; Nucleoprotein; CTN Strain; Antigencity; Expression

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	C. E. Rupprecht, C. A. Hanlon and T. Hemachudha, “Rabies Re-Examined,” The Lancet Infectious Diseases, Vol. 2, No. 6, 2002, pp. 327-343. http://dx.doi.org/10.1016/S1473-3099(02)00287-6
[2]	“Rabies,” Biotech Business Week, Vol. 4, No. 12, 2004, p. 448.
[3]	W. H. Wunner and J. K. Larson, “The Molecular Biology of Rabies Viruses,” Reviews of infectious diseases, Vol. 10, Suppl. 4, 1988, pp. 771-784. http://dx.doi.org/10.1093/clinids/10.Supplement_4.S771
[4]	W. H. Wunner, “The Chemical Composition and Molecular Structure of Rabies Viruses,” In: G. M. Baer, Ed., The Natural History of Rabies. CRC Press, Boca Raton, 1991, pp. 31-67.
[5]	K. K. Conzelmann, “Nonsegmented Negative-Strand RNA Viruses: Genetics and Manipulation of Viral Genomes,” Annual Review of Genetics, Vol. 32, 1998, pp. 123-162. http://dx.doi.org/10.1146/annurev.genet.32.1.123
[6]	A. Flamand, T. J. Wiktor and H. Koprowshi, “Use of Hybridoma Monoclonal Antibodies in the Detection of Antigenic Differences Between Rabies and Rabies-related Virus Proteins. I. The Nucleocapsid Protein,” Journal of General Virology, Vol. 48, No. 1, 1980, pp. 97-104. http://dx.doi.org/10.1099/0022-1317-48-1-97
[7]	H. C. Ertl, B. Dietzschold, M. Gore, L. Otvos Jr., J. K. Larson, W. H. Wunner and H. Koprowski, “Induction of Rabies Virus-Specific T-Helper Cells by Synthetic Peptides That Carry Dominant T-Helper Cell Epitopes of the Viral Ribonucleoprotein,” Journal of Virology, Vol. 63, No. 7, 1989, pp. 2885-2892.
[8]	H. Goto, N. Minamoto, H. Ito, M. Sugiyama, T. Kinjo, K. Mannen, K. Mifune and A. Kawai, “Nucleotide Sequence of the Nucleoprotein Gene of the RC·HL Strain of Rabies Virus, a Seed Strain Used for Animal Vaccine Production in Japan,” Virus Genes, Vol. 8, No. 2, 1994, pp. 91-97. http://dx.doi.org/10.1007/BF01703607
[9]	A. Kawai, J. Anzai, Y. Honda, K. Morimoto, K. Takeuchi, T. Kohno, K. Wakisaka, H. Goto and N. Minamoto, “Monoclonal Antibody #5-2-26 Recognizes the Phosphatase-Sensitive Epitope of Rabies Virus Nucleoprotein,” Microbiology & Immunology, Vol. 41, No. 1, 1997, pp. 33-42.
[10]	Q. Ouyang, C. Tang, J. Zheng and J. Zhu, “Cloning and Sequencing of Nucleoprotein Gene of Chinese Rabies Virus Vaccine Strain (5aG),” Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi, Vol. 11, No. 1, 1997, pp. 13-15.
[11]	H. Goto, N. Minamoto, H. Ito, N. Ito, M. Sugiyama, T. Kinjo and A. Kawai, “Mapping of Epitopes and Structural Analysis of Antigenic Sites in the Nucleoprotein of Rabies Virus,” Journal of General Virology, Vol. 81, No. 1, 2000, pp. 119-127.
[12]	N. Minamoto, H. Tanaka and M. Hoshida, “Linear and Conformation-Dependent Antigenic Sites on the Nucleoprotein of Rabies Virus,” Microbiology and Immunology, Vol. 38, No. 6, 1994, pp. 449-455.
[13]	S. Inoue Y. Sato, H. Hasegawa, A. Noguchi, A. Yamada, T. Kurata and T. Iwasaki, “Cross-Reactive Antigenicity of Nucleoproteins of Lyssaviruses Recognized by a Monospecific Antirabies Virus Nucleoprotein Antiserum on Paraffin Sections of Formalin-Fixed Tissues,” Pathology International, Vol. 53, No. 8, 2003, pp. 525-533. http://dx.doi.org/10.1046/j.1440-1827.2003.01511.x
[14]	F. Cliquet, L. M. Mcelhinney, A. Servat, J. M. Boucher, J. P. Lowings, T. Godard, K. L. Mansfield and A. R. Fooks, “Development of a Qualitative Indirect ELISA for the Measurement of Rabies Virus-Specific Antibodies from Vaccinated Dogs and cats,” Journal of Virological Methods, Vol. 117, No. 1, 2004, pp. 1-8. http://dx.doi.org/10.1016/j.jviromet.2003.12.001
[15]	S. Inoue, Y. Motoi, T. Kashimura, K. Ono and A. Yamada, “Safe and Easy Monitoring of Anti-Rabies Antibody in Dogs Using His-Tagged Recombinant N-Protein,” Japanese Journal of Infectious Diseases, Vol. 56, No. 4, 2003, pp. 158-160.