Feeding Reduces Serum Concentration of Endogenous Bioactive (1-84), but Not Total Intact Parathyroid Hormone in Female Sprague-Dawley Rats

Abstract

Background: Interpretation of parathyroid hormone (PTH) measurements requires an understanding of its structural conformation. PTH reflects calcium intake and vitamin D status but is commonly measured in the fasted state using 2nd generation assays, known to cross-react with PTH 7-84 fragments, which is not observed with 3rd generation assays. The objective was to determine if plasma PTH in the fed and non-fed state differ while controlling for diurnal rhythm and the generation of PTH assay. Methods: Blood was sampled, 2 d apart, from Sprague-Dawley rats (30 males and 30 females) in both fed and non-fed states at 20 wk of age (weight: 470.2 ± 23.2 g) for measurement of ionized calcium (iCa), total intact (INT) and bioactive 1-84 (BIO) PTH. Differences between groups were tested using a GLIMMIX model with sex and feeding state as fixed effects and individual rats as a random effect. Results: Females had a lower iCa than males (F: 1.43 ± 0.01 vs M: 1.46 ± 0.01 mmol/L, P = 0.03). In males and females, there was no difference between fed and non-fed groups when PTH was assessed using the INT PTH assay (M Fed: 21.6 ± 1.3 vs M Non-fed: 22.6 ± 2.3 pmol/L, P = 0.59; F Fed: 19.3 ± 1.5 vs F Non-fed: 22.8 ± 2.9 pmol/L, P = 0.39). However, in females only, PTH measured using the BIO PTH, was significantly lower in the fed group versus the non-fed (Fed: 8.4 ± 0.8 vs Non-fed: 16.7 ± 3.4 pmol/L, P = 0.05). Conclusions:

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DeGuire, J. and Weiler, H. (2013) Feeding Reduces Serum Concentration of Endogenous Bioactive (1-84), but Not Total Intact Parathyroid Hormone in Female Sprague-Dawley Rats. Open Journal of Nephrology, 3, 61-65. doi: 10.4236/ojneph.2013.31010.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] T. Fujita, S. Ohgitani and Y. Fujii, “Overnight Suppression of Parathyroid Hormone and Bone Resorption Markers by Active Absorbable Algae Calcium. A Double-Blind Crossover Study,” Calcified Tissue International, Vol. 60, No. 6, 1997, pp. 506-512. doi:10.1007/s002239900272
[2] W. R. McKane, S. Khosla, K. S. Egan, S. P. Robins, M. F. Burritt and B. L. Riggs, “Role of Calcium Intake in Modulating Age-Related Increases in Parathyroid Function and Bone Resorption,” The Journal of Clinical Endocrinology and Metabolism, Vol. 81, No. 5, 1996, pp. 1699-1703. doi:10.1210/jc.81.5.1699
[3] H. A. Weiler, S. Austin, S. Fitzpatrick-Wong, E. Nitschmann, N. Bankovic-Calic, R. Mollard, H. Aukema and M. Ogborn, “Conjugated Linoleic Acid Reduces Parathyroid Hormone in Health and in Polycystic Kidney Disease in Rats,” American Journal of Clinical Nutrition, Vol. 79, No. S6, 2004, pp. S1186-S1189.
[4] H. A. Weiler, S. Fitzpatrick and S. C. Fitzpatrick-Wong, “Dietary Conjugated Linoleic Acid in the cis-9, trans-11 Isoform Reduces Parathyroid Hormone in Male, but Not Female Rats,” Journal Nutritional Biochemistry, Vol. 19, No. 11, 2008, pp. 762-769. doi:10.1016/j.jnutbio.2007.09.010
[5] P. Gao and P. D’Amour, “Evolution of the Parathyroid Hormone (PTH) Assay—Importance of Circulating PTH Immunohetero-geneity and of Its Regulation,” Clinical Laboratory, Vol. 51, No. 1-2, 2005, pp. 21-29.
[6] C. Ruh, N. Doyle, P. Oldfield, P. Bednarek and S. Y. Smith, “Effects of Fasting on Endogenous Parathyroid Hormone (PTH) Levels in Cynomolgus Monkeys,” Journal of Bone and Mineral Research, Vol. 25, No. S1, 2010, pp. S363-S502.
[7] Canadian Council on Animal Care, “Guide to the Care and Use of Experimental Animals,” Bradda Printing Services Inc., Ottawa, 1993.
[8] P. G. Reeves, “Components of the AIN-93 Diets as Improvements in the AIN-76A Diet,” Journal of Nutrition, Vol. 127, No. S5, 1997, pp. S838-S841.
[9] E. Slatopolsky, J. Finch, P. Clay, D. Martin, G. Sicard, G. Singer, P. Gao, T. Cantor and A. Dusso, “A Novel Mechanism for Skeletal Resistance in Uremia,” Kidney International, Vol. 58, No. 2, 2000, pp. 753-761.
[10] P. Divieti, M. R. John, H. Juppner and F. R. Bringhurst, “Human PTH-(7-84) Inhibits Bone Resorption in Vitro via Actions Independent of the Type 1 PTH/PTHrP Receptor,” Endocrinology, Vol. 143, No. 1, 2002, pp. 171-176. doi:10.1210/en.143.1.171
[11] H. Yamashita, P. Gao, T. Cantor, T. Futata, T. Murakami, S. Uchino, S. Watanabe, H. Kawamoto, M. Fukagawa and S. Noguchi, “Large Carboxy-Terminal Parathyroid Hormone (PTH) Fragment with a Relatively Longer Half-Life than 1-84 PTH is Secreted, Directly From the Parathyroid Gland in Humans,” European Journal of Endocrinology, Vol. 149, No. 4, 2003, pp. 301-306. doi:10.1530/eje.0.1490301
[12] M. Inaba, K. Nakatsuka, Y. Imanishi, M. Watanabe, Y. Mamiya, E. Ishimura and Y. Nishizawa, “Technical and Clinical Characterization of the Bio-PTH (1-84) Immunochemiluminometric Assay and Comparison with a Second-Generation Assay for Parathyroid Hormone,” Clinical Chemistry, Vol. 50, No. 2, 2004, pp. 385-390. doi:10.1373/clinchem.2003.026831
[13] A. Schlemmer and C. Hassager, “Acute Fasting Diminishes the Circadian Rhythm of Biochemical Markers of Bone Resorption,” European Journal of Endocrinology, Vol. 140, No. 4, 1999, pp. 332-337. doi:10.1530/eje.0.1400332
[14] W. D. Fraser, F. C. Logue, J. P. Christie, D. A. Cameron, D. S. Oreilly and G. H. Beastall, “Alteration of the Circadian-Rhythm of Intact Parathyroid-Hormone Following a 96-Hour Fast,” Clinical Endocrinology, Vol. 40, No. 4, 1994, pp. 523-528.
[15] M. S. Calvo, R. Kumar and H. Heath, “Elevated Secretion and Action of Serum Parathyroid-Hormone in Young-Adults Consuming High Phosphorus, Low Calcium Diets Assembled from Common Foods,” Journal of Clinical Endocrinology & Metabolism, Vol. 66, No. 4, 1988, pp. 823-829. doi:10.1210/jcem-66-4-823
[16] W. D. Fraser, F. C. Logue, J. P. Christie, S. J. Gallacher, D. Cameron, D. S. J. O’Reilly, G. H. Beastall and I. T. Boyle, “Alteration of the Circadian Rhythm of Intact Parathyroid Hormone and Serum Phosphate in Women with Established Postmenopausal Osteoporosis,” Osteoporosis International, Vol. 8, No. 2, 1998, pp. 121-126. doi:10.1007/BF02672507
[17] F. C. Logue, W. D. Fraser, D. S. Oreilly and G. H. Beastall, “The Circadian-Rhythm of Intact Parathyroid-Hormone (1-84) and Nephrogenous Cyclic Adenosine-Monophosphate in Normal Men,” Journal of Endocrinology, Vol. 121, No. 1, 1989, pp. R1-R3. doi:10.1677/joe.0.121R001
[18] M. E. Markowitz, S. Arnaud, J. F. Rosen, M. Thorpy and S. Laximinarayan, “Temporal Interrelationships between the Circadian-Rhythms of Serum Parathyroid-Hormone and Calcium Concentrations,” Journal of Clinical Endocrinology & Metabolism, Vol. 67, No. 5, 1988, pp. 1068-1073. doi:10.1210/jcem-67-5-1068
[19] M. S. Calvo, R. Kumar and H. Heath, “Persistently Elevated Parathyroid-Hormone Secretion and Action in Young-Women after 4 Weeks of Ingesting High Phosphorus, Low Calcium Diets,” Journal of Clinical Endocrinology & Metabolism, Vol. 70, No. 5, 1990, pp. 1334-1340. doi:10.1210/jcem-70-5-1334
[20] E. Slatopolsky, A. Brown and A. Dusso, “Pathogenesis of Secondary Hyperparathyroidism,” Kidney International, Vol. 73, 1999, pp. S14-S19. doi:10.1046/j.1523-1755.1999.07304.x
[21] E. Slatopolsky and J. A. Delmez, “Pathogenesis of Secondary Hyperparathyroidism,” Nephrology Dialysis Transplantation, Vol. 11, 1996, pp. 130-135. doi:10.1093/ndt/11.supp3.130
[22] E. M. Brown, M. Pollak, C. E. Seidman, J. G. Seidman, D. Riccardi and S. C. Hebert, “Seminars in Medicine of the Beth-Israel-Hospital, Boston—Calcium-Ion-Sensing Cell-Surface Receptors,” New England Journal of Medicine, Vol. 333, No. 4, 1995, pp. 234-240. doi:10.1056/NEJM199507273330407
[23] E. Reiss, J. M. Canterbury, M. A. Bercovitz and E. L. Kaplan, “Role of Phosphate in Secretion of Parathyroid Hormone in Man,” Journal of Clinical Investigation, Vol. 49, No. 11, 1970, pp. 2146-2149. doi:10.1172/JCI106432
[24] E. Slatopolsky, A. Dusso and A. J. Brown, “The Role of Phosphorus in the Development of Secondary Hyperparathyroidism and Parathyroid Cell Proliferation in Chronic Renal Failure,” American Journal of the Medical Sciences, Vol. 317, No. 6, 1999, pp. 370-376. doi:10.1097/00000441-199906000-00004
[25] T. Navehmany, M. M. Friedlaender, H. Mayer and J. Silver, “Calcium Regulates Parathyroid-Hormone Messenger Ribonucleic-Acid (Messenger-Rna), but Not Calcitonin Messenger-Rna in Vivo in the Rat—Dominant Role of 1,25-Dihydroxyvitamin-D,” Endocrinology, Vol. 125, No. 1, 1989, pp. 275-280. doi:10.1210/endo-125-1-275
[26] C. E. Hotchkiss, R. Brommage, M. Du and C. P. Jerome, “The Anesthetic Isoflurane Decreases Ionized Calcium and Increases Parathyroid Hormone and Osteocalcin in Cynomolgus Mon-keys,” Bone, Vol. 23, No. 5, 1998, pp. 479-484. doi:10.1016/S8756-3282(98)00124-0
[27] L. E. Mallette, “The Parathyroid Polyhormones—New Concepts in the Spectrum of Peptide-Hormone Action,” Endocrine Reviews, Vol. 12, No. 2, 1991, pp. 110-117. doi:10.1210/edrv-12-2-110
[28] P. D’amour, J. H. Brossard, A. Rakel, L. Rousseau, C. Albert and T. Cantor, “Evidence that the Amino-Terminal Composition of Non-(1-84) Parathyroid Hormone Fragments Starts before Position 19,” Clinical Chemistry, Vol. 51, No. 1, 2005, pp. 169-176. doi:10.1373/clinchem.2004.040485
[29] P. D’amour, J. H. Brossard, L. Rousseau, L. Nguyen- Yamamoto, E. Nassif, C. Lazure, D. Gauthier, J. R. Lavigne and R. J. Zahradnik, “Structure of Non-(1-84) PTH Fragments Secreted by Parathyroid Glands in Primary and Secondary Hyperparathyroidism,” Kidney International, Vol. 68, No. 3, 2005, pp. 998-1007. doi:10.1111/j.1523-1755.2005.00493.x
[30] A. Rakel, J. H. Brossard, J. V. Patenaude, C. Albert, E. Nassif, T. Cantor, L. Rousseau and P. D’Amour, “Overproduction of an Amino-Terminal Form of PTH Distinct from Human PTH(1-84) in a Case of Severe Primary Hyperparathyroidism: Influence of Medical Treatment and Surgery,” Clinical Endocrinology, Vol. 62, No. 6, 2005, pp. 721-727.

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