Synthesis and Development of Gd³⁺-ALGDG₂-C595 as MR Imaging Contrast Agent

Mehdi Mirzaei, Mohammadali Mohagheghi, Daryoush Shahbazi-Gahrouei

Cancer Institute Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Phys- ics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

DOI: 10.4236/jbnb.2013.41004                

Abstract

Magnetic Resonance imaging (MR imaging) as a powerful non-invasive modality is of high global interest for early cancer detection. The aim of this study was the synthesis of nanodendrimer and its conjugate with monoclonal antibody C595 against breast cancer cell, followed by its chelating with gadolinium for its magnetic property. First, anti-MUC-1 monoclonal antibody C595 was coupled to a biodegradable biocompatible Anionic Linear Globular Dendrimer G₂ (having polyethylene glycol core and citric acid shell). Then prepared nanocomplex loaded by gadolinium to make novel agent of functional MR imaging. Anticancer effects and MR imaging parameters of the prepared nanoconjugate was investigated under in vitro conditions doing performing several studies such as evaluation of monoclonal antibody C595 binding to mucine-1 (MUC-1) cell, its purification, size of nanoconjugate and relaxivity measurements. The obtained data showed a powerful relaxations as well as selective MUC-1 antigen binding to the cell. Based on the findings from the present research Gd³⁺-ALGDG₂-C595 nano-probe may be a potential breast molecular imaging and therapeutic agent. However, further investigations by in vivo studies and clinical trials are in the pipeline.

Keywords

Gd³⁺-ALGDG₂; Mucine-1; Monoclonal Antibody C595; MR Imaging; in Vitro

Conflicts of Interest

The authors declare no conflicts of interest.

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