Platelet-Derived Microparticles in Patients with Colorectal Cancer

Violetta Dymicka-Piekarska, Mariusz Gryko, Alina Lipska, Aleksandra Korniluk, Elżbieta Siergiejko, Halina Kemona

Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, Bialystok, Poland.
Department of General and Gastroenterological Surgery, Medical University of Bialystok, Bialystok, Poland.
Regional Centre for Transfusion Medicine, Bialystok, Poland..

DOI: 10.4236/jct.2012.326115                

Abstract

Microparticles (MPs) are membrane vesicles released by platelets during activation or apoptosis. PMPs stimulate coagulation and cell adhesion to the vascular endothelium, both of which being important in the development of cancer progression. We investigated the correlation between microparticles and cancer advancement. The percentage of PMPs was measured by flow cytometry in 22 colorectal cancer patients and in 20 healthy subjects. The patients were divided into two groups according to the presence or lack of lymph node metastases. The percentage of PMPs was significantly higher in patients than in healthy subjects (p < 0.001). We observed a positive correlation between PMPs and the presence of lymph node metastases (r = 0.63, p < 0.003). This high correlation may suggest that PMPs are associated with lymph node involvement and cancer progression.

Keywords

Microparticles; Platelets; Colorectal Cancer

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	M. D. Shah, A. L. Bergeron, J. F. Dong, et al., “Flow Cytometric Measurement of Microparticles: Pitfalls and Protocol Modifications,” Platelets, Vol. 19, No. 5, 2008, pp. 365-372. doi:10.1080/09537100802054107
[2]	A. R. Beaudoin and G. Grondin, “Shedding of Vesicular Material from the Cell Surface of Eucaryotic Cells: Different Cellular Phenomena”, Biochimica et Biophysica Acta, Vol. 1071, No. 3, 1991, pp. 203-219. doi:10.1016/0304-4157(91)90014-N
[3]	A. Weber, H. O. Koppen and K. Schror, “Platelet-Derived Micropartocles Stimulate Coronary Artery Smooth Muscle Cell Mitogenesis by a PDGF-Independent Mechanism,” Thrombosis Research, Vol. 98, No. 5, 2000, pp. 461-466. doi:10.1016/S0049-3848(00)00192-4
[4]	M. Baj-Krzyworzeka, M. Majka, D. Pratico, et al., “Platelet-Derived Microparticles Stimulate Proliferation, Survival, Adhesion, and Hemotaxis of Hematopoietic Cells,” Experimental Hematology, Vol. 30, No. 5, 2002, pp. 450-459. doi:10.1016/S0301-472X(02)00791-9
[5]	J. Simak and M. P. Gelderman, “Cell Membrane Microparticles in Blood and Blood Products: Potentially Pathogenic Agents and Diagnostic Markers”, Transfusion Medicine Reviews, Vol. 20, No. 1, 2006, pp. 1-26. doi:10.1016/j.tmrv.2005.08.001
[6]	J. Rak, “Microparticles in Cancer,” Seminars in Thrombosis and Hemostasis, Vol. 36, No. 8, 2010, pp. 888-906. doi:10.1055/s-0030-1267043
[7]	N. Ogata, M. Imaizumi, S. Nomura, et al., “Increased Levels of Platelet-Derived Microparticles in Patients With Diabetic Retinopathy,” Diabetes Research and Clinical Practice, Vol. 68, No. 3, 2005, pp. 193-201. doi:10.1016/j.diabres.2004.10.010
[8]	B. Toth, S. Liebhardt, K. Steinig, et al., “Platelet-Derived Microparticles and Coagulation Activation in Breast Cancer Patients,” Journal of Thrombosis and Haemostasis, Vol. 100, No. 4, 2008, pp. 663-669.
[9]	M. C. Martinez, A. Tesse, F. Zobairi, et al., “Shed Membrane Microparticles from Circulating and Vascular Cells in Regulation Vascular Function,” American Journal of Physiology—Heart and Circulatory Physiology, Vol. 288, 3, 2005, pp. 1004-1009. doi:10.1152/ajpheart.00842.2004
[10]	D. Castellana, F. Toti and J. M. Freyssinet, “Membrane Microvesicles: Macromessengers in Cancer Disease and Progression,” Thrombosis Research, Vol. 125, No. 2, 2010, pp. S84-S88. doi:10.1016/S0049-3848(10)70021-9
[11]	D. Varon and E. Shai, “Role of Platelet-Derived Microparticles in Angiogenesis and Tumor Progression,” Discovery Medicine, Vol. 8, No. 43, 2009, pp. 237-241.
[12]	H. K. Kim, K. S. Song, J. H. Chung, et al., “Platelet Microparticles Induce Angiogenesis in Vitro,” British Journal of Haematology, Vol. 124, No. 3, 2004, pp. 376-384. doi:10.1046/j.1365-2141.2003.04773.x
[13]	S. P. Ardoin, J. C. Shanahan and D. S. Pisetsky, “The Role of Microparticles in Inflammation and Thrombosis,” Scandinavian Journal of Immunology, Vol. 66, No. 1, 2007, pp. 159-165. doi:10.1111/j.1365-3083.2007.01984.x
[14]	A. Janowska-Wieczorek, M. Wysoczynski, J. Kijowski, et al., “Microvesicles Derived from Activated Platelets Induce Metastasis and Angiogenesis in Lung Cancer,” International Journal of Cancer, Vol. 113, No. 5, 2005, pp. 752-760. doi:10.1002/ijc.20657
[15]	R. Flaumenhaft, A. T. Mairuhu and J. E. Italiano, “Platelet and Megakaryocyte-Derived Microparticles,” Seminars in Thrombosis and Hemostasis, Vol. 36, No. 8, 2010, pp. 881-887. doi:10.1055/s-0030-1267042