Sensitivity assay of polymerase chain reaction for detection of Canine Parvo Virus infection in dogs

Prashant Sharma, Amit Rastogi, Kartikaye Kukreti, Partap Singh Narwal

Central Military Veterinary Laboratory, Meerut, Uttarpradesh, India.

DOI: 10.4236/ojcd.2012.23009                

Abstract

A polymerase chain reaction was performed using re-ported primers for detection of Canine Parvo virus (CPV) in the stool sample obtained from repository. The PCR primers were specific to VP1/VP2 gene of CPV. Sensi-tivity assay of PCR detection was performed by making dilutions of CPV positive DNA extracted from fecal sample, carrying out PCR for each dilution and visualiz-ing amplicons in ethidium bromide stained agarose gel under UV radiation. Study was valuable in determining the efficiency of PCR. The sensitivity of PCR in present study was determined to be equivalent to detection of .00 2pg/μl of CPV DNA. The study was conducted to analyze the variation, sensitivity and repeatability.

Keywords

CPV; Canine Parvo Virus; PCR; Polymerase Chain Reaction; Sensitivity

Conflicts of Interest

The authors declare no conflicts of interest.

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