ABC> Vol.2 No.3, August 2012
Downloads: 751     Views: 2,538

Effectiveness of gelatin solidification for unfrozen preservation and transportation of astrocytes

DownloadDownload as PDF (Size:359KB) Full-Text HTML PP. 238-242   DOI: 10.4236/abc.2012.23029

ABSTRACT

We herein studied a non-cryogenic cell preservation and transportation method. We found that astrocytes can be preserved in an unfrozen state at 4℃ (without medium exchange), while maintaining good condition in comparison with cryopreservation for the periods of 5 - 7 days. The gelatin solidification method can also prevent decreases in survival rate by preventing damage to astrocytes during transportation. Therefore, the gelatin solidification method at 4℃ may be a useful choice for short-term preservation and transportation.

KEYWORDS


Cite this paper

Ishibashi, N. , Sotome, S. , Nakajima, K. and Shimizu, A. (2012) Effectiveness of gelatin solidification for unfrozen preservation and transportation of astrocytes. Advances in Biological Chemistry, 2, 238-242. doi: 10.4236/abc.2012.23029.

References

[1] Acker, J.P., Elliott, J.A.W. and McGann, L.E. (2001) Intercellular ice propagation: Experimental evidence for ice growth through membrane pores. Biophyicals Journal, 81, 1389-1397. doi:10.1016/S0006-3495(01)75794-3
[2] Acker, J.P. and McGann, L.E. (2002) Innocuous intracellular ice improves the survival of frozen cells. Cell Transplant, 11, 563-571.
[3] Mazur, P. (1984) Freezing of living cells: Mechanisms and implications. American Journal of Physiology, 247, C125-C147.
[4] David, P.E. (1976) Long-term preservation of cells and tissues: A review. Journal of Clinical Pathology, 29, 271- 285. doi:10.1136/jcp.29.4.271
[5] Acker, J.P. and McGann, L.E. (2003) Protective effect of intracellular ice during freezing? Cryobiology, 46, 197- 202. doi:10.1016/S0011-2240(03)00025-7
[6] Hubálek, Z. (2003) Protectants used in the cryopreservation of microorganisms. Cryobiology, 46, 205-229. doi:10.1016/S0011-2240(03)00046-4
[7] Lozina-Lozinsk, L.K. (1965) Survival of some insects and cells following intracellular ice formation. Federation proceedings, 15, S206-S211.
[8] McGann, L.E. (1978) Differing actions of penetrating and nonpenetrating cryoprotective agents. Cryobiology, 15, 382-390. doi:10.1016/0011-2240(78)90056-1
[9] Odintsova, N., Kiselev. K., Sanina, N. and Kostetsky, E. (2001) Cryopreservation of primary cell cultures of marine invertebrates. CryoLetters, 22, 299-310.
[10] Baust, J.M., Buskirk, R.V. and Baust, J.G. (2000) Cell viability improves following inhibition of cryopreservation-induced apoptosis. In Vitro Cellular & Developmental Biology—Animal, 36, 262-270. doi:10.1290/1071-2690(2000)036<0262:CVIFIO>2.0.CO;2
[11] Paynter, S., Cooper, A., Thomas, N. and Fuller, B. (1996) Cryopreservation of multicellular embryos and tissues. In: Karow, A. and Critser, J., Eds., Reproductive Tissue Banking, Academic Press, New York, 1997.
[12] Polge. C., Smith, A.U. and Parkes, A.S. (1949) Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature, 164, 666. doi:10.1038/164666a0
[13] Frankland, J.C., Latter, P.M. and Poskitt, J.M. (1995) A laboratory guide to solid microbiology: Some general principles and practice. Medewood Research and Development Paper, 115, 1-79.
[14] Kim, N.S. and Kim, S.J. (1991) Isolation and cultivation of microvascular endothelial cells from rat lungs: Effects of gelatin substratum and serum. Yonsei Medical Journal, 32, 303-314.
[15] Araque, A., Parpura, V., Sanzgiri, R.P. and Haydon, P.G. (1999) Tripartite synapses: Glia, the unacknowledged partner. Trends in Neurosciences, 22, 208-215. doi:10.1016/S0166-2236(98)01349-6
[16] Kolb, B and Whishaw, I.Q. (2008) Fundamentals of human neuropsychology. 6th Edition, Worth, New York.

comments powered by Disqus

Copyright © 2014 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.