Ultrastructural Analysis of the Ontogenetic Development of Shoot Induced from Embryonic Axes of Costa Rican Bean Varieties (Phaseolus vulgaris L.) under in Vitro Conditions by Scanning Electronic Microscopy

Marisol Jiménez, Andrés Gatica, Ethel Sánchez, Marta Valdez

Centro de Investigación en Estructuras Microscópicas (CIEMic), Universidad de Costa Rica, San Pedro de Montes de Oca, Costa Rica.
Laboratorio de Biotecnología y Transformación Genética de Plantas, Escuela de Biología, Universidad de Costa Rica, San Pedro de Montes de Oca, Costa Rica.

DOI: 10.4236/ajps.2012.34058                

Abstract

Common bean (Phaseolus vulgaris L.) is an economic important crop and one of the major grain legumes for human consumption in Latin America, Africa and Asia. A morphological study of shoot induced from embryonic axes development in four Costa Rican bean varieties (Brunca, Huetar, Guaymi and Bribri) cultivated on MS media with or without 5 mg·L^–1 de N⁶-benzylaminopurine (BAP) by scanning electron microscopy (SEM) was developed in the present work. Micrographs showed similarities and differences in the ultrastructure of the apical dome, epidermal surface, stomata and different types of trichomes in the varieties cultivated on organogenesis media. Genotypes with advantageous morphological characteristics for genetic transformation, in particular an exposed apical dome, were identified. This work will contribute to the optimization of the in vitro regeneration of four common bean varieties.

Keywords

Phaseolus vulgaris L; Bean; In Vitro Organogenesis; Shoot; Scanning Electron Microscopy (SEM)

Conflicts of Interest

The authors declare no conflicts of interest.

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