A Comparative Testing of Cucumber mosaic virus (CMV)-Based Constructs to Generate Virus Resistant Plants

Xinqiu Tan, Deyong Zhang, Charlotte Wintgens, Peter Willingmann, Günter Adam, Cornelia Heinze

Biocentre Klein-Flottbek and Botanical Garden, University of Hamburg, Ham-burg, Germany.
Hunan Plant Protection Institute, Changsha, China.

DOI: 10.4236/ajps.2012.34055                

Abstract

Among the viruses Cucumber mosaic virus (CMV) has been rated worldwide as one of the five most important viruses infecting vegetable species. CMV is a tripartite virus with high sequence variability, classified into three subgroups with 80% to 97% identical nucleotides in their coat protein. Due to the absence of natural resistance CMV is the plant virus with longest history in genetic engineering using pathogen induced approaches. However, the transformation and regeneration for some very important crops like chili is difficult. Therefore it will be an advantage to screen in model plants for gene constructs which might be independent of the target of final transformation and other parameters having an influence on the efficiency of a biotechnological approach. In our study we compared the resistance for all combinations of five different antiviral constructs, two different transformation vectors and two model host plants. From these approaches we identified the most effective construct which might also be applicable to transform eventually chili plants.

Keywords

Cucumber mosaic virus; Transgenic Plant; Resistance; Comparison

Conflicts of Interest

The authors declare no conflicts of interest.

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