New Method for Optimization and Simultaneous Determination of Sparfloxacin and Non Steroidal Anti-Inflammatory Drugs: Its In-Vitro Application

Somia Gul, Najma Sultana, Muhammad Saeed Arayne, Sana Shamim, Mahwish Akhtar

College of Pharmacy, Dow University of Health Sciences, Karachi, Pakistan.
Faculty of Pharmacy, Jinnah University for Women, Karachi, Pakistan.
United Biotech (Pvt) Ltd., Karachi, Pakistan.

DOI: 10.4236/ajac.2012.34045                

Abstract

A simple reversed phase HPLC method was developed and validated for the simultaneous determination of sparfloxacin (SPFX), diclofenac sodium, meloxicam, ibuprofen, flurbiprofen, naproxen and mefenemic acid in a relatively short time with high linearity in bulk material, pharmaceutical formulations and human serum. Purospher STAR C₁₈ (250 × 4.6 mm, 5 μm) column was utilized with mobile phase, methanol and water (90:10, v/v pH 2.70 adjusted by phosphoric acid), was delivered at a flow rate of 1.5 mL.min^–1. Eluent was monitored using UV detector at 240 nm. The proposed method is specific, accurate (98.42% - 102.75%), precise (intra-day and inter-day variation 0.011% - 1.85%) and linear (R2 > 0.999) with in the desired range 0.15 - 40 μg.mL^–1 and the detection and quantification limit was 1.19E+08 – 0.150 μg.mL^–1 and 3.62E+08 – 0.4574 μg.mL^–1 respectively for SPFX and NSAIDs. The analysis of variance (ANOVA) and student’s t-test were applied to verify the results. The anticipated method is applicable to routine analysis of SPFX and NSAIDs in pharmaceutical formulations as well as in human serum samples. It has also applied on interaction of SPFX with NSAIDs.

Keywords

Sparfloxacin; NSAIDs; HPLC; Interactions; ANOVA

Conflicts of Interest

The authors declare no conflicts of interest.

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