Effect of antigens on colony forming efficiency of stromal clonogenic cells and expression of cytokine genes in primary cultures of bone marrow and spleen of mice
U.F. Gorskaya, T.A. Danilova, M.V. Mezentseva, I.M. Shapoval, V.G. Nesterenko
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DOI: 10.4236/abb.2011.25046   PDF    HTML     3,318 Downloads   6,681 Views   Citations

Abstract

Presence of microbial cells preparation (HCL – extract of group A streptococcus) in primary cell cultures of bone marrow (BMCC) and spleen (SCC) of CBA mice induced the expression mRNA of proinflammatory cytokines genes: IL-2, IL-6 and IL-8 in BMCC; in SCC cultures mRNA IL-1 β and IL-6 appeared and mRNA IL-4 disappeared. Injection of S. typhimurium antigen complex to mice CBA increased 5 times colony forming efficiency (CFE) and, respectively, content of stromal precursor cells (CFU-F) in femur bone marrow and 9 times in spleen of these animals with maximum at the first day. In both primary BMCC and SCC from immunized animals expression of proinflammatory cytokines genes IL-1 β, IL-6, IL-8 and TNF- α was revealed - in BMCC on 1 - 3 day after immunization, and in SCC – up to 15 days. In CBA mice injected with polyvinylpyrrolidone (PVP) CFE and number of CFU-F in BMCC and SCC increased 1, 9 and 1, 8 times only and expression of genes IL-6, IL-8 and TNF- was observed only on the first day. In СВА/N xid mice there was neither increase CFU-F in the corresponding organs, nor expression of proinflammatory cytokines genes in primary cultures. The data suggest the possibility of positive participation of stromal cells in the development on of immune response in organism. Degree of activation of stromal tissue in immunized mice, apparently, correlates with the degree of immune response, supposing a close relationship between stromal tissue and immune system.

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Gorskaya, U. , Danilova, T. , Mezentseva, M. , Shapoval, I. and Nesterenko, V. (2011) Effect of antigens on colony forming efficiency of stromal clonogenic cells and expression of cytokine genes in primary cultures of bone marrow and spleen of mice. Advances in Bioscience and Biotechnology, 2, 315-319. doi: 10.4236/abb.2011.25046.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Rasmussen I., Ringen O., Sundberg B. et al (2005) Mesenchymal stem cells inhibit lymphocyte proliferation by mitogens and alloantigens by different mechanisms, Exp. Cell. Res., 305, 33-41.
[2] Pevsner-Fisher M., Morad V., Cohen-Sfady M. et al (2007) Toll-like receptors and their ligands control mesenchymal stem cells function, Blood, 109, 1422-1432.
[3] Kim D.H., Yoo K.H., Choi K.S. et al. (2005) Gene expression profile of cytokine and growth factor during differentiation of bone marrow derived mesenchymal stem cell, Cytokine, 31, 119-126.
[4] Friedenstein A.Ya., Latzinic N.V., Gorskaya Yu.F. et al (1992) Bone marrow stromal colony formation requires stimulation by haemopoietic cells, Bone and Mineral, 18, 199-213.
[5] Сhomezynski P., Sacchi N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction, Anal. Biochem., 162, 156-159.
[6] Gelder C.M., Thomas P.S., Yates D.H. et al (1995) Cytokine expression in normal, atopic and asthmatic subjects using the combination of sputum induction and the polymerase chain reaction, Thorax, 50, 1033-1037.
[7] Lin Y., Zhang M., Barnes P.F. (1998) Chemokine production by a human alveolar epithelial cell line in response to Mycobacterium tuberculosis, Infect. Immun., 66, 1121- 1126.
[8] Yamamura M, Uyemura K.,Deans R.J. et al (1991) Defining protecting responses to pathogens: cytokine profiles in leprosy lesions, Science, 254, 277-279.
[9] Gaede K.I., Mamat U., Schlaak M. et al (1999) Analysis of differentially regulated mRNAs in monocytic cells induced by in vitro stimulation, J. Mol. Med., 77, 847- 852.
[10] Gorskaya Yu.F., Mezentseva M.V., Shapoval I.V., Danilova T.A.et al (2009) The influence of bacterial cell preparation , IFN- ,and MIF on cytokine gene expression in passage stromal fibroblasts of human bone marrow in vitro and cells of mouse bone marrow and spleen in primary cultures, New Horizons in Allergy, Asthma & Immunology MEDIMOND International Proceedings Proceedings of the 11 World Asthma & Cord Forum SP (Russia), 205-208.
[11] Chaylakyan R.K., Gerasimov Yu.V., Kuralesova A.I. et al (2001) Izvestiya АS, Proliferative and differential potentions of individual clones of stromal cell precursors in bone marrow, 6, 682- 687.
[12] Waterman R Waterman RS, Tomchuk SL et al (2010) A New Mesenchymal Stem Cell Paradigm: Polarization into a Pro-Inflammatory MSC-1 or an Immunosupressive VSC-2 Phenotipe, PLOS ONE, 5, 1-14.
[13] Liotta F., Angeli R., Cosmi L. et al (2008) Toll-like receptors 3 and 4 are expressed by human bone marrow derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling, Stem Cells, 26, 279-289.
[14] Sidorova E.V. What do we know today about B lymphocytes? (2006) Uspehi sovremennoy biologii, 3, 227-241.
[15] Prior L, Pierson S, Woodland R.T., Riggs J.(1994) Rapid restoration of B-cell function in xid mice by intravenous transferof peritoneal cavity B-cells, Immunology, 83, 180-183.

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