Response of Cowpea Genotypes to Drought Stress in Uganda

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DOI: 10.4236/ajps.2017.84050    174 Downloads   256 Views  

ABSTRACT

Moisture stress is a challenge to cowpea production in the drought prone areas of eastern and north eastern Uganda, with yield losses of up to 50% reported. Genotypes grown by farmers are not drought tolerant. This study was therefore, undertaken at Makerere University Agricultural Research Institute Kabanyolo to identify cowpea genotypes tolerant to drought. Thirty cowpea accessions comprising of Ugandan landraces and released varieties, Brazilian lines, Makerere University breeding lines, elite IITA germplasm and seven IITA drought tolerant lines as checks were screened for drought tolerance at vegetative and reproductive stages. The experiment was designed as a 2 × 37 factorial and laid out in a split-plot arrangement, 37 genotypes of cowpea at two soil moisture stress levels (T1, no stress and T2, severe stress) with all factorial combinations replicated two times in a screen house. The genotypes showed considerable variability in tolerance to drought. Genotypes were significantly different for chlorophyll content (P ≤ 0.01), efficiency of photosystem II (P ≤ 0.05), non-photochemical quenching (P ≤ 0.05), recovery (P ≤ 0.01), delayed leaf senescence (P ≤ 0.01), grain yield (P ≤ 0.01), 100 seed weight (P ≤ 0.05), number of pods per plant and number of seeds per pod (P ≤ 0.001). There was a high significant positive correlation between chlorophyll content and efficiency of photosystem II (r = 0.75, P ≤ 0.001) implying that chlorophyll content and efficiency of photosystem II could be used as efficient reference indicators in the selection of drought tolerant genotypes. Genotypes SECOW 5T, SECOW 3B, SECOW 4W, WC 30 and MU 24 C gave relatively high yields under stress and no stress conditions, maintained above mean chlorophyll content, efficiency of photosystem II and had good recovery scores from stress and thus were tolerant to drought stress induced at both vegetative and reproductive stages.

Cite this paper

Mwale, S. , Ochwo-Ssemakula, M. , Sadik, K. , Achola, E. , Okul, V. , Gibson, P. , Edema, R. , Singini, W. and Rubaihayo, P. (2017) Response of Cowpea Genotypes to Drought Stress in Uganda. American Journal of Plant Sciences, 8, 720-733. doi: 10.4236/ajps.2017.84050.

References

[1] Agbicodo, E.M., Fatokun, C.A., Muranaka, S., Visser, R.G.F. and Linden van der, C.G. (2009) Breeding Drought Tolerant Cowpea: Constraints, Accomplishments, and Future Prospects. Euphytica, 167, 353-370.
https://doi.org/10.1007/s10681-009-9893-8
[2] Sariah, J. (2010) Enhancing Cowpea (Vigna unguiculata L.) Production through Insect Pest Resistant Line in East Africa. PhD Thesis, University of Copenhagen, Copenhagen.
[3] Orawu, M., Obuo, J. and Omadi, R. (2015) Distribution and Detection of Cowpea Viruses Infecting Cowpea in Uganda. American Journal of Plant Science, 6, 574-581.
https://doi.org/10.4236/ajps.2015.65062
[4] Adipala, E. (1994) Cowpea Improvement in Uganda: Current Status of Diseases and Pest Management Half Year Report for the Period September 1993-March 1994. Makerere University, Kampala.
[5] Rusoke, D.G. and Rubaihayo, P. (1994) The Influence of Some Crop Protection Management Practices on Yield Stability of Cowpeas. African Journal of Crop Science, 2, 143-148.
[6] Bisikwa, J. (2011) McKnight Foundation Collaborative Crops Research Project No. 09-480 Improving Food Security through Participatory Development of High Yielding and Pests Resistant Cowpea Varieties in Uganda Annual Progress Report—Narrative.
[7] Hall, A.E. (2012) Phenotyping Cowpea for Adaptation to Drought. Frontiers in Physiology, 3, 1-8.
https://doi.org/10.3389/fphys.2012.00155
[8] Bernstein, L., Bosch, O., Canziani, Z., Chen, R. and Davidson, C.O. (2008) Climate Change 2007: Synthesis Report: An Assessment of the Intergovernmental Panel on Climate Change. Intergovernmental Panel on Climate Change, Geneva.
[9] Alidu, M., Atokple, I. and Akromah, R. (2013) Genetic Analysis of Vegetative Stage Drought Tolerance in Cowpea. Greener Journal of Agricultural Sciences, 3, 481-496.
[10] Boyer, J.S. and McPherson, H.G. (1975) Physiology of Water Deficits in Cereal Crops. Advances in Agronomy, 27, 1-27.
https://doi.org/10.1016/S0065-2113(08)70006-3
[11] Wang, Z.L. and Huang, B.R. (2004) Physiological Recovery of Kentucky Bluegrass from Simultaneous Drought and Heat Stress. Crop Science, 44, 1729-1736.
https://doi.org/10.2135/cropsci2004.1729
[12] Nkouannessi, M. (2005) The Genetic, Morphological and Physiological Evaluation of African Cowpea Genotypes. MSc Thesis, University of the Free State, Bloemfontein.
[13] Hamidou, F., Zombre, G. and Braconnier, S. (2007) Physiological and Biochemical Responses of Cowpea Genotypes to Water Stress under Glasshouse and Field Conditions. Journal of Agronomy and Crop Science, 193, 229-237.
https://doi.org/10.1111/j.1439-037X.2007.00253.x
[14] Anantharaju, P. and Muthiah, A.R. (2008) Screening for Drought Tolerance in Cowpea. Legume Research, 31, 283-285.
[15] Hayatu, M. and Mukhtar, F.B. (2010) Physiological Responses of Some Drought Resistant Cowpea Genotypes to Water Stress. Bayero Journal of Pure and Applied Sciences, 3, 69-75.
[16] Mashilo, J. (2013) Response of Dual-Purpose Landraces to Water Stress. MSc Thesis, University of Kwazulu Natal, Durban.
[17] Khaki, N. (2014) Evaluation of Malawi Pigeon Pea (Cajanus Cajan L) Accessions for Tolerance to Moisture Stress and Superior Agronomic Traits in Uganda. MSc Thesis, Makerere University, Kampala.
[18] Bisikwa, J., Ekere, W., Kawooya, R., Biruma, M. and Okello, D. (2014) Farmers Guide to Sustainable Cowpea Production in Uganda.
[19] Muchero, W., Roberts, P.A., Diop, N.N., Drabo, I., Cisse, N., Close, T.J., Muranaka, S., Boukar, O. and Ehlers, J.D. (2013) Genetic Architecture of Delayed Senescence, Biomass, and Grain Yield under Drought Stress in Cowpea. PLoS ONE, 8, e70041.
https://doi.org/10.1371/journal.pone.0070041
[20] Chiulele, R.M. (2010) Breeding Cowpea for Improved Drought Tolerance in Mozambique. PhD Thesis, University of Kwazulu Natal, Durban.
[21] Gwathmey, C.O. and Hall, A.E. (1992) Adaptation to Midseason Drought of Cowpea Genotypes with Contrasting Senescence Traits. Crop Science, 32, 773-778.
https://doi.org/10.2135/cropsci1992.0011183X003200030039x
[22] Muchero, W., Ehlers, J.D. and Roberts, P.A. (2008) Seedling Stage Drought Induced Phenotypes and Drought Responsive Genes in Diverse Cowpea Genotypes. Crop Science, 48, 541.
https://doi.org/10.2135/cropsci2007.07.0397
[23] International Board for Plant Genetic Resources (IBPGR) (1983) Cowpea Descriptors. Rome.
[24] Mai-Kodomi, Y., Singh, B.B., Myers, O., Yopp, J.H., Gibson, P.J. and Terao, T. (1999) Two Mechanisms of Drought Tolerance in Cowpea. Indian Journal of Genetics & Plant Breeding, 59, 309-316.
[25] Pungulani, L.L.M., Milner, J.P., Warren, M.W. and Banda, M. (2013) Improvement of Leaf Wilting Scoring System in Cowpea: From Qualitative Scale to Quantitative Index. Australian Journal of Crop Science, 7, 1262-1269.
[26] Kuhlgert, S., Austic, G., Zegarac, R., Bonsu, I.O., Hoh, D., Chilvers, M.I., Roth, M.G., TerAvest, D. and Kramer, D.M. (2016) MultispeQ Beta: A Tool for Large Scale Plant Phenotyping Connected to the Open PhotosynQ Network. Royal Society Open Science, 3, Article ID: 160592.
https://doi.org/10.1098/rsos.160592
[27] Krause, G.H. and Weis, E. (1991) Chlorophyll Fluorescence and Photosynthesis: The Basis. Annual Review of Plant Physiology and Plant Molecular Biology, 42, 313-349.
[28] Parida, A.K., Dagaonkar, V.S. and Phalak, M.S. (2007) Alterations in Photosynthetic Pigments, Protein and Osmotic Components in Cotton Genotypes Subjected to Short-Term Drought Stress Followed by Recovery. Plant Biotechnology Reports, 1, 37-48.
https://doi.org/10.1007/s11816-006-0004-1
[29] Balouchi, H.R. (2010) Screening Wheat Parents of Mapping Population for Heat and Drought Tolerance, Detection of Wheat Genetic Variation. World Academy of Science, Engineering & Technology, No. 42, 210.
[30] Nyachiro, J.M., Briggs, K.G., Hoddinott, J. and Johnson-Flanagan, A.M. (2001) Chlorophyll Content, Chlorophyll Fluorescence and Water Deficit in Spring Wheat. Cereal Research Communications, 29, 135-142.
[31] Fotovat, R., Valizadeh, M. and Toorehi, M. (2007) Association between Water-Use Efficiency Components and Total Chlorophyll Content (SPAD) in Wheat (Triticum aestivum L.) under Well-Watered and Drought Stress Conditions. Journal of Food, Agriculture and Environment, 5, 225-227.
[32] Smirnoff, N. (1995) Antioxidant Systems and Plant Response to the Environment. In: Smirnoff, V., Ed., Environment and Plant Metabolism: Flexibility and Acclimation, BIOS Scientific Publishers, Oxford, 217-243.
[33] Kaiser, W.M. (1987) Effects of Water Deficit on Photosynthetic Capacity. Physiologia Plantarum, 71, 142-149.
https://doi.org/10.1111/j.1399-3054.1987.tb04631.x
[34] Pungulani, L.L.M. (2014) Exploring the Genetic Potential of Locally Adapted Germplasm for Drought Tolerance: A Case for Cowpea (Vigna unguiculata (L.)) Walp) from Malawi. PhD Thesis, Massey University, Palmerston North.
[35] Anyia, A.O. and Herzog, H. (2004) Genotypic Variability in Drought Performance and Recovery in Cowpea under Controlled Environment. Journal of Agronomy and Crop Science, 190, 151-159.
https://doi.org/10.1111/j.1439-037X.2004.00096.x
[36] Turk, K.J. and Hall, A.E. (1980) Drought Adaptation of Cowpea. II. Influence of Drought on Plant Water Status and Relation with Seed Yield. Agronomy Journal, 72, 421-427.
https://doi.org/10.2134/agronj1980.00021962007200030005x
[37] Masaya, P. (1991) Adaptation to Photoperiod and Temperature. In: Breeding for Drought Tolerance by Integrative Design: The Case of Common Bean in Ethiopia. PhD Thesis, 445-500.

  
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