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Muscle and adipose tissue biopsy in older adults with type 2 diabetes

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DOI: 10.4236/jdm.2011.13005    5,369 Downloads   10,119 Views   Citations

ABSTRACT

We describe a method for obtaining skeletal muscle and subcutaneous adipose tissue in a single percutaneous needle biopsy procedure. Biopsies were performed in a double-blind randomized, sham-exercise controlled clinical trial of power training in 103 older adults with type 2 diabetes. In total 110 biopsies (73.3%) were performed in the first 50 participants at 3 time points. Muscle samples were obtained in 102/110 biopsies (92.7%); adipose samples were obtained in 72/110 biopsies (65.5%); both tissue types were obtained in 65/110 biopsies (59.1%). The average weight of muscle samples was 131±92 mg and the median weight of adipose samples was 51 mg (range 3 to 412 mg). No adverse effects of biopsies were reported other than mild transient pain or light-headedness. This is one of the first reports of both adipose and muscle tissue obtained during the same biopsy, and the first to describe in detail sampling technique, yield and safety.

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Wang, Y. , Simar, D. , Anderberg, K. , Mavros, Y. , Kay, S. , Zhao, R. , Baune, B. , O’Sullivan, A. , Singh, N. and Singh, M. (2011) Muscle and adipose tissue biopsy in older adults with type 2 diabetes. Journal of Diabetes Mellitus, 1, 27-35. doi: 10.4236/jdm.2011.13005.

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