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Application of Renneted Skim Milk as a Fat Mimetics in Nonfat Yoghurt

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DOI: 10.4236/fns.2011.26077    5,876 Downloads   10,993 Views   Citations


For a long time, fermented dairy products have been consumed for human’s nutrition and health. Recently, consumption of whole dairy products, such as full fat yoghurt, has declined due to the awareness of probable harmful effects of fat on consumers’ health, resulting change of market interest in favor of low or non fat dairy products. It is a challenge for food scientists to produce a suitable fat substitute providing the functionality of the missing fat. The present investigation was carried out to examine the effect of 0 to 50% renneted skim milk (RSM) as a fat mimetic upon the physico-chemical, physical, rheological and sensory characteristics of nonfat yoghurt in comparison to control samples (full fat and skim yoghurt). By increasing RSM content, the chemical characteristics (titratable acidity, pH as well as the content of fat and total protein) of yoghurts did not alter except decrease of total solid in sample with 50% RSM. The yoghurts with more content of RSM exhibited higher b*and a* values, while the highest L* value, viscosity, water holding capacity (WHC), firmness and lowest syneresis were found in sample containing 30% RSM. It seems that new interaction in gel network of yoghurts containing RSM, exhibited greater ability to bind water than control skim yoghurt. In addition, probably new bridges between milk proteins (interacting partially hydrolyzed casein with casein) can increase the number of bounds between protein particles leading to lower syneresis. However, excessive RSM content (higher than 30%) did not increase the L* value, viscosity and WHC of samples probably because of too much aggregation of casein. Sensory results revealed that sample containing 30% RSM was exactly alike to full fat yoghurt in terms of overall sensory attributes. New formulation of palatable skim yoghurt was developed using 3% RSM successfully.

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M. Nouri, H. Ezzatpanah and S. Abbasi, "Application of Renneted Skim Milk as a Fat Mimetics in Nonfat Yoghurt," Food and Nutrition Sciences, Vol. 2 No. 6, 2011, pp. 541-548. doi: 10.4236/fns.2011.26077.


[1] A. L. B. Penna, S. Gurram and G. V. B. Canovas, “Effect of High Hydrostatic Pressure Processing on Rheological and Textural Properties of Probiotic Low-Fat Yogurt Fermented by Different Starter Cultures,” Journal of Food Process Engineering, Vol. 29, No. 5, 2006, pp. 447-461. doi:10.1111/j.1745-4530.2006.00076.
[2] A. Y. Tamime, and R. K. Robinson, “Tamime and Robinson’s Yoghurt,” 3rd Edition, Woodhead Publishing Limited and CRC press, New York, 2007, pp. 322-332.
[3] C. S. Brennan and C. M. Tudorica, “Carbohydrate-Based Fat Replacers in the Modification of the rheological, Textural and Sensory Quality of Yoghurt: Comparative Study of the Utilization of Barley Betaglucan, Guar Gum and Inulin,” Inrnational Journal of Food and Technology, Vol. 43, No. 5, 2006, pp. 824-833. doi:10.1111/j.1365-2621.2007.01522.x
[4] S. L. Amaya-Llano, A. L. Martinez-Alegria, J. J. Zazueta-Moreles and F. Martinez-Bustos, “Acid Thinned Jicama and Maize Starches as Fat Substitute in Stirred Yogurt,” LWT―Food Science and Technology, Vol. 41, 2007, pp. 1274-1281.
[5] M. Guzman-Gonzales, F. Morais and L. Amigo, “Influence of Skimmed Milk Concentrate Replacement by dry Dairy Products in a Low-Fat Set-Type Yoghurt Model System. Use of caseinates, Co-Precipitate and Blended Dairy Powder,” Journal of the Science of Food and Agriculture, Vol. 80, No. 4, 2000, pp. 433-438. doi:10.1002/(SICI)1097-0010(200003)80:4<433::AID-JSFA545>3.0.CO;2-B
[6] V. V. Mistry and H. N. Hassan, “Manufacture of Nonfat Yogurt from a High Milk Protein Powder,” Journal of Dairy Science, Vol. 75, No. 4, 1992, pp. 947-957. doi:10.3168/jds.S0022-0302(92)77835-7
[7] S. M. Fiszman, M. A. Lluch and A. Salvador, “Effect of Addition of Gelatin on Microstructure of Acidic Milk Gels and Yoghurt and on Their Rheological Properties,” International Dairy Journal, Vol. 9, No. 12, 1999, pp. 895-901. doi:10.1016/S0958-6946(00)00013-3
[8] S. Y. Xu, D. W. Stanley, H. D. Goff, V. J. Davidson and M. LeMarguer, “Hydrocolloid/Milk Gel Formation and Properties,” Journal of Food Science, Vol. 57, No. 1, 1992, pp. 96-102. doi:10.1111/j.1365-2621.1992.tb05433.x
[9] H. S. Ramaswamy and S. Basak, “Pectin and Raspberry Concentrate Effects on the Rheology of Stirred Commercial Yogurt,” Journal of Food Science, Vol. 57, No. 2, 1992, pp. 357-360. doi:10.1111/j.1365-2621.1992.tb05494.x
[10] M. Guven, K. Yasar, O. B. Karaca and A. A. Hayaloglu, “The Effect of Inulin as a Fat Replacer on the Quality of Set-Type Low-Fat Yogurt Manufacture,” International Journal of Dairy Technology, Vol. 58, No. 3, 2005, pp. 180-184. doi:10.1111/j.1471-0307.2005.00210.x
[11] D. Ozer, S. Akin and B. Ozer, “Effect of Inulin and Lactulose on Survival of Lactobacillus Acidophilus LA-5 and Bifidobacterium Bifidum BB-02 in Acidophilus-Bifidus Yoghurt,” Food Science and Technology International, Vol. 11, No. 1, 2005, pp. 19-24. doi:10.1177/1082013205051275
[12] E. Fernandez-Garcia and J. U. McGregor, “Fortification of Sweetened Plain Yogurt with Insoluble Dietary Fiber,” Chemistry and Materials Science, Vol. 204, No. 6, 1997, pp. 433-437. doi:10.1007/s002170050108
[13] N. Sahan, K. Yasar and A. A. Hayaloglu, “Physical Chemical and Flavour Quality of Non-Fat Yogurt as Affected by a β-Glucan Hydrocolloidal Composite during Storage,” Journal of Food Hydrocolloids, Vol. 22, No. 7, 2008, pp. 1291-1297. doi:10.1016/j.foodhyd.2007.06.010
[14] Y. Ma, C. G. Cai, J. Wang and D. W. Sun, “Enzymatic Hydrolysis of Corn Starch for Producing Fat Mimetics,” Journal of Food Engineering, Vol. 73, No. 3, 2006, pp. 297-303. doi:10.1016/j.jfoodeng.2005.01.023
[15] K. A. Schmidt, T. J. Herald and K. A. Khatib, “Modified Wheat Starches Used as Stabilizers in Set-Style Yogurt,” Journal of Food Quality, Vol. 24, No. 5, 2001, pp. 421-434. doi:10.1111/j.1745-4557.2001.tb00620.x
[16] H. W. Modler, M. E. Larmond, C. S. Lin, D. Froehlich and D. B. Emmons, “Physical and Sensory Properties of Yogurt Stabilized with Milk Proteins,” Journal of Dairy Science, Vol. 66, No. 3, 1983, pp. 422-429. doi:10.3168/jds.S0022-0302(83)81809-8
[17] R. I. Dave and P. Shah, “The Influence of Ingredient Supplementation on the Textural Characteristics of Yogurt,” Australian Journal of Dairy Technology, Vol. 53, No. 3, 1998, pp. 180-184.
[18] A. Puvanethiran, R. P. W. Williams and M. A. Augustin, “Strucure and Viscoelastic Properties of Set Yoghurt with Altered Casein to Whey Protein Ratios,” Journal of International Dairy, Vol. 12, No. 4, 2002, pp. 383-391. doi:10.1016/S0958-6946(02)00033-X
[19] F. Remeuf, S. Mohammed, I. Sodini and J. P. Tissier, “Preliminary Observations on the Effects of Milk Fortification and Heating on Microstructure and Physical Properties of Stirred Yogurt,” International Dairy Journal, Vol. 13, No. 9, 2003, pp. 773-782. doi:10.1016/S0958-6946(03)00092-X
[20] V. Westergaad, “Milk Powder Technology Evaporation and Spray Drying,” NIRO A/S, Copenhagen, 1994, p. 89.
[21] H. Ezzatpanah, M. Nouri, S. Abbasi, M. Aminafshar, H. Behmadi, “Chemical, Physical and Sensory Properties of Renneted Skim Milk,” 9th International Conference of Food Science and Technology, Zhejiang, 27-29 May 2011.
[22] AOAC, “Official Methods of Analysis,” 15th Edition, Horwith, Washington, 1995.
[23] N. Trachoo and V. V. Mistry. “Application of Ultrafiltered Sweet Buttermilk and Sweet Buttermilk Powder in the Manufacture of Nonfat and Low Fat Yogurts,” Journal of Dairy Science, Vol. 81, No. 12, 1998, pp. 3163-3171. doi:10.3168/jds.S0022-0302(98)75882-5
[24] A. Y. Tamime, E. Barrantes and A. M. Sword, “The Effect of Starch Based Fat Substitutes on the Microstructure Of Set-Style Yogurt Made from Reconstituted Skimmed Milk Powder,” Journal of Society of Dairy Technology, Vol. 49, No. 1, 1996, pp. 1-10. doi:10.1111/j.1471-0307.1996.tb02612.x
[25] M. K. Keogh and B. T. O’kennedy, “Rheology of Stirred Yogurt as Affected by Added Milk Fat, Protein and Hydrocolloids,” Journal of Food Science, Vol. 63, No. 1, 1998, pp. 108-112. doi:10.1111/j.1365-2621.1998.tb15687.x
[26] M. Kalab, P. A. Wojtas and B. E. P. Todd, “Development of Microstructure in Set-Style Nonfat Yogurt,” Journal of Food Microstructure, Vol. 2, 1983, pp. 51-66.
[27] E. P. Clunies, Y. Kakuda, K. Mullen, D. R. Aronott and J. M. Deman, “Physical properties of Yogurt: A Comparison of Vat versus Continuous Heating Systems of Milk,” Journal of Dairy Science, Vol. 69, No. 10, 1986, pp. 2593-2603. doi:10.3168/jds.S0022-0302(86)80706-8
[28] O. Sandoval-Castilla, C. Lobato-Calleros, E. Aguirre-Mandujano and J. Vernon-Cartet, “Microstructure and Texture of Yogurt as Influenced by Fat Replacers,” International Dairy Journal, Vol. 14, No. 2, 2004, pp. 151-159. doi:10.1016/S0958-6946(03)00166-3
[29] S. M. Kitessa, S. K. Gulati, G. C. Simos, J. R. Ashes, T. W. Scott, E. Fleck and P. C. Wynn, “Supplementation of Grazing Dairy Cows with Rumen-Protected Tuna oil Enriches Milk Fat with n-3 Fatty Acids without Affecting Milk Production or Sensory Characteristics,” British Journal of Nutrition, Vol. 91, No. 3, 2003, pp. 271-277.
[30] H. Stone and J. L. Sidel, “Sensory Evaluation Practices,” 3rd Edition, Elsevier Academic Press, Waltham, 2004, pp. 38-50.
[31] A. E. Labropoulos, A. Lopez and J. K. Palmer, “Apparent Viscosity of Milk and Cultured Yogurt Thermally Treated by UHT and Vat Systems,” Journal of Food Protection, Vol. 44, 1981, pp. 874-876.
[32] S. M. Schellhaass and H. A. Morris, “Rheological and Scanning Electron Microscopic Examination of Skim Milk Gels Obtained by Fermenting with Ropy and Non-Ropy Strains of Lactic Acid Bacteria,” Food Microstructure, Vol. 4, 1985, pp. 279-287.
[33] D. G. Dalgleish and A. J. R. Law, “pH-Induced Dissociation of Bovine Casein Micelles. I. Analysis of liberated Caseins,” Journal of Dairy Research, Vol. 55, No. 4, 1988, pp. 529-538. doi:10.1017/S0022029900033306
[34] P. Mitschka, “Simple Conversion of Brookfield R. V. T. Readings into Viscosity Functions,” Rheologica Acta, Vol. 21, No. 2, 1982, pp. 207-209. doi:10.1007/BF01736420
[35] T. Amatayakul, F. Sherkat and N. P. Shah, “Syneresis In Set Yogurt as Affected by EPS Starter Cultures and Levels of Solids,” International Journal of Dairy Technology, Vol. 59, No. 3, 2006, pp. 216-221. doi:10.1111/j.1471-0307.2006.00264.x
[36] V. L. Landege, “Quality of Yogurt Supplemented with Whey Protein Concentrate and Effect of Whey Protein Denaturation,” MSC Dissertation, Mumbai University, Mumbai, 2005, pp. 40-68.

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