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Developmental Dynamics of Wheat (Triticum aestivum L.) Microspores under Culture

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DOI: 10.4236/abb.2015.612072    4,147 Downloads   4,497 Views  

ABSTRACT

Doubled haploid production via microspore culture is a technique known to accelerate crop breeding by shortening the breeding cycle through achieving homozygosity in one generation. Prior research observed that some embryogenic microspores aborted their development before reaching the embryoid stage. Such embryogenic abortion reduces embryoid yield, making microspore cultures less efficient. The present research aims at identifying stages during which microspore development is susceptible to embryogenic abortion. Information gained through delineation of the developmental dynamics of microspores in culture could be used to improve the efficiency of microspore culture. Embryogenic microspores were isolated from stress-treated wheat (Triticum aestivum L.) tillers and cultured in liquid medium. The development of embryogenic microspores was monitored over a 35 day period. At day 7, 10, 14, 21, 28, and 35, the developing microspores were counted and categorized into multicellular structures, pre-embryoids, immature embryoids and mature embryoids. The results showed that 44% - 62% of embryogenic microspores halted their development before the mature embryoid stage. Of these aborted embryogenic microspores, 21% - 33% terminated as multicellular structures, 16% - 19% arrested their development as pre-embryoids, and 7% - 10% halted development as immature embryoids. Identifying factors that are responsible for embryogenic abortion and finding remedy to the issue will help improve the efficiency of doubled haploid production.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Zheng, M. , Bieren, K. and Griggs, R. (2015) Developmental Dynamics of Wheat (Triticum aestivum L.) Microspores under Culture. Advances in Bioscience and Biotechnology, 6, 693-701. doi: 10.4236/abb.2015.612072.

References

[1] Guha, S. and Maheshwari, S.C. (1964) In Vitro Production of Embryos from Anthers of Datura. Nature, 204, 497.
http://dx.doi.org/10.1038/204497a0
[2] Gustafson, V.D., Baenziger, P.S., Wright, M.S., Stroup, W.W. and Yen, Y. (1995) Isolated Wheat Microspore Culture. Plant Cell, Tissue and Organ Culture, 42, 207-213.
http://dx.doi.org/10.1007/BF00034239
[3] Hu, T.C., Ziauddin, A., Simion, E. and Kasha, K.J. (1995) Isolated Microspore Culture of Wheat (Triticum aestivum L.) in a Defined Media, I. Effects of Pretreatment, Isolation Methods, and Hormones. In Vitro Cellular & Developmental Biology—Plant, 31, 79-83.
http://dx.doi.org/10.1007/BF02632241
[4] Liu, W.G., Zheng, M.Y., Polle, E. and Konzak, C.F. (2002) Highly Efficient Doubled-Haploid Production in Wheat (Triticum aestivum L.) via Induced Microspore Embryogenesis. Crop Science, 42, 686-692.
http://dx.doi.org/10.2135/cropsci2002.0686
[5] Touraev, A., Indrianto, A., Wratschko, I., Vicente, O. and Heberle-Bors, E. (1996) Efficient Microspore Embryogenesis in Wheat (Triticum aestivum L.) Induced by Starvation at High Temperature. Sexual Plant Reproduction, 9, 209-215.
http://dx.doi.org/10.1007/BF02173100
[6] Zheng, M.Y., Liu, W., Weng, Y., Polle, E. and Konzak, C.F. (2001) Culture of Freshly Isolated Wheat (Triticum aestivum L.) Microspores Treated with Inducer Chemicals. Plant Cell Reports, 20, 685-690.
http://dx.doi.org/10.1007/s00299-001-0393-0
[7] Sunderland, N. (1974) Anther Culture as a Means of Haploid Induction. In: Kasha, K.J., Ed., Haploids in Higher Plants-Advances and Potential, University of Guelph, Guelph, 91-122.
[8] Zheng, M.Y. (2003) Microspore Culture in Wheat (Triticum aestivum)—Double Haploid Production via Induced Embryogenesis. Plant Cell, Tissue and Organ Culture, 73, 213-230.
http://dx.doi.org/10.1023/A:1023076213639
[9] Kasha, K.J., Ziauddin, A. and Cho, U.H. (1990) Haploids in Cereal Improvement: Anther and Microspore Culture. In: Gustafson, J.P., Ed., Gene Manipulation in Plant Improvement II, Plenum Press, New York, 213-235.
http://dx.doi.org/10.1007/978-1-4684-7047-5_11
[10] Reynolds, T.L. (1993) A Cytological Analysis of Microspores of Triticum aestivum (Poaceae) during Normal Ontogeny and Induced Embryogenic Development. American Journal of Botany, 80, 569-576.
http://dx.doi.org/10.2307/2445374
[11] Reynolds, T.L. (1997) Pollen Embryogenesis. Plant Molecular Biology, 33, 1-10.
http://dx.doi.org/10.1023/A:1005748614261
[12] Touraev, A., Ilham, A., Vicente, O. and Heberle-Bors, E. (1996) Stress-Induced Microspore Embryogenesis in Tobacco: An Optimized System for Molecular Studies. Plant Cell Reports, 15, 561-565.
http://dx.doi.org/10.1007/BF00232453
[13] Bruins, M.B.M., Rakoczy-Trojanowska, M. and Snijders, C.H.A. (1996) Isolated Microspore Culture in Wheat (Triticum aestivum L.): The Effect of Co-Culture of Wheat or Barley Ovaries on Embryogenesis. Cereal Research Communications, 24, 401-408.
[14] Zheng, M.Y., Weng, Y., Liu, W. and Konzak, C.F. (2002) The Effect of Ovary-Conditioned Medium on Microspore Embryogenesis in Common Wheat (Triticum aestivum L.). Plant Cell Reports, 20, 802-807.
http://dx.doi.org/10.1007/s00299-001-0411-2
[15] Hu, T.C. and Kasha, K.J. (1997) Improvement of Isolated Microspore Culture of Wheat (Triticum aestivum L.) through Ovary Co-Culture. Plant Cell Reports, 16, 520-525.
[16] Zheng, M.Y., Liu, W., Weng, Y., Polle, E. and Konzak, C.F. (2003) Production of Doubled Haploids in Wheat (Triticum aestivum L.) through Microspore Embryogenesis Triggered by Inducer Chemicals. In: Maluszynski, Kasha, Forster and Szarejko, Eds., Doubled Haploids Production in Crop Plants, A Manual, Chapter 2, Kluwer Academic Publisher, Dordrecht, 83-94.
[17] Letarte, J., Simon, E., Miner, M. and Kasha, K. (2006) Arabinogalactans and Arabinogalactan-Proteins Induce Embryogenesis in Wheat (Tricitum aestivum L.) Microspore Culture. Plant Cell Reports, 24, 691-698.
[18] Hunter, C.P. (1988) Plant Regeneration from Microspores of Barley (Hordeum vulgare). MS Thesis, Wye College, University of London, London.
[19] Roberts-Oehlschlager, S. and Dunwell, S.M. (1990) Barley Anther Culture: Pretreatment on Mannitol Stimulates Production of Microspore-Derived Embryos. Plant Cell Tissue Organ Culture, 20, 235-240.

  
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