Efficient Extraction of Agarose from Red Algae Using Ionic Liquids

Abstract

We explored the possibility of using ionic liquids (ILs) as medium for efficient extraction of agarose via dissolution of red algae under varying conditions of heating or microwave irradiation. As compared to conventional methods, a very high extraction yield of good quality agarose (as high as 39 wt%) could be achieved depending upon the nature of used IL and applied experimental conditions. Purity of extracted agarose was confirmed from various spectral and analytical techniques, such as 1H and 13C NMR, FTIR, circular dichroism (CD), gel permeation chromatography (GPC) and thermogravimetric analysis (TGA). The physicochemical properties, such as gelling or melting temperature, viscosity and gel strength of extracted agarose hydrogels have been measured and compared with the agarose obtained from similar source reported in the literature. ILs were recovered after the extraction of agarose and were reused for further extraction experiments. % Recycling and extraction ability of recycled ILs in different cycles have been measured. The developed extraction process of utilizing ILs as medium is easy, simple and highly efficient as compared to the conventional methods of agarose extraction from algae.

Share and Cite:

Trivedi, T. and Kumar, A. (2014) Efficient Extraction of Agarose from Red Algae Using Ionic Liquids. Green and Sustainable Chemistry, 4, 190-201. doi: 10.4236/gsc.2014.44025.

1. Introduction

Utilization of natural biopolymers has attracted increasing attention because of the consumption and over-ex- ploitation of non-renewable resources, such as coal, fossil fuel and oil. The development of green processing of biomass (the most abundant bio renewable material on earth) is urgent from the viewpoints of both sustainability and environmental protection. Polysaccharides are widely distributed in nature and have been regarded as structural materials and as suppliers of water and energy [1] . Polysaccharide chemistry is presently receiving renewed attention due to the increase of role of biopolymers in various biochemical, biomedical and industrial applications. Due to fibril and rigid structure, the efficient dissolution and extraction of polysaccharides using organic solvents involve significant amounts of energy. Also, because of toxic, volatile, flammable and corrosive nature of organic solvents such processes are neither sustainable nor environmental friendly [2] . Therefore, the design of safe and environmentally benign extraction processes has an increasingly important role in the development of clean manufacturing processes and in the remediation of sites contaminated by an older generation of manufacturing technologies [3] . Utilization of comparatively greener solvents with high solvating ability towards biomass could be an alternative for the development of novel and efficient extraction processes of biopolymers from the plant species.

Ionic liquids (ILs) are compounds that contain only ions and have melting point below 100˚C [4] . ILs have been recognized as “greener” solvents compared to organic solvents mainly due to their negligible vapor pressure, high thermal and chemical stability, and their capacity for dissolving numerous polar and non-polar compounds. Due to high solvating ability, ILs are being used in different separation and extraction processes. After Swastloski [5] reporting the ILs as potential cellulose solvents in 2002, many research groups explored the utilization of these materials for dissolution and regeneration of biomass and some good reviews have been recently appeared [6] -[8] . In recent years the extraction of various chemicals or biopolymers from biomass has also been carried using ILs [9] -[30] . For example, the valuable chemicals, such as free fatty acid from soybean oil, shikimic acid from Ginkgo biloba leaves or star anise seeds, lactones from Ligusticum chuanxiong, tannins from plant materials and caffeine from bioresources guaraná seeds have been extracted employing different types of ILs [9] -[14] . As far as extraction of biopolymers from biomass utilizing ILs is concerned, various biomass fractions have been obtained by direct dissolution of wood biomass in ILs [15] -[19] . Lignin, which is another valuable biomass fraction, is also extracted from different wood sources utilizing ILs [20] -[25] . Besides the extraction of cellulose, hemicellulose or lignin from wood sources, there are other biopolymers, such as keratin [26] , pectin [27] , chitin [28] [29] and suberin [30] which have been successfully isolated from the bioresources using ILs.

Marine microalgae is another category of biomass that contains many useful and valuable chemicals, like lipid, oil, polysaccharides, fuels and chemical feedstock, etc. in abundance, but its poor solubility in conventional solvents restricts efficient extraction of these valuable chemicals. There are few reports where ILs have been used as extraction medium for the recovery of valuable chemicals from algae. Teixeira et al. [31] reported the extraction of fuels and other valuable chemicals from microalgae using ILs, while Kim et al. [32] investigated the extraction of lipids from algal biomass using ILs and methanol mixture. Ohno’s research group has reported the extraction of polysaccharides from bran using phosphonate or phosphinate ILs in very short time at mild temperature [33] . Recently, the same research group reported dissolution of wet and saliferous marine microalgae (WSM) using polar ionic liquids without any heating [34] .

Herein, we reported the extraction of agarose from red microalgae (Rhodophyta), mainly Gracilaria dura obtained from west coast of India using different ILs under varying conditions of heating and examined the effect of nature of different cations or anions of ILs on extraction efficiency. Extracted agarose has been characterized by means of spectroscopic and analytical methods. Physico-chemical properties of extracted agarose and its hydrogels have been measured and compared with agarose extracted earlier from the similar sources using conventional methods [35] . ILs were efficiently recovered and recycled in various experiments.

2. Experimental

2.1. Materials

ILs: 1-ethyl-3-methylimidazolium acetate, [Emim] [OAc], choline acetate, [Ch] [OAc] and 1-ethyl-3-methyl imidazolium diethyl phosphate, [Emim] [Dep] were purchased from Sigma Aldrich having higher than 99 wt% purity. Prior to use ILs were dried and stored in glove box. The red algae Gracilaria dura from family Gracilariaceae, Rhodophyta were collected from west Indian coastal regions. Algae were dried and crushed before use in experiments. Molecular structures of the repeating unit of agarose monomer and the constituent ions of the ILs used in this study are shown in Figure 1.

Figure 1. (a) Molecular structures of repeating unit of agarose monomer and the constituent ions of the ILs; (b) 1-Ethyl-3-methylimidazolium acetate, [Emim] [OAc]; (c) 1-Ethyl-3-methylimidazolium diethyl phosphate, [Emim] [Dep]; and (d) Choline acetate, [Ch] [OAc].

2.2. Methods

2.2.1. Extraction of Agarose from Algae Using ILs

Extraction of agarose from red algae using ILs was carried out according to the representative steps shown in Scheme 1. Extraction process was performed in 10 ml beakers with controlled temperature conditions such as temperature or micro wave (MW) heating coupled with normal heating. Typically, 0.5 g of dried and powdered algae was treated with 10.0 g of preheated ILs in beakers. The mixture containing (algae + ILs) was stirred at 80/100˚C for 2 h or MW treatment for 2 min at 3 s pulse was given prior to the normal heating. In order to reduce the viscosity of IL-algae solution, 10% boiled water was added. Hot water treated mixture was stirred until free flow homogenization, and was filtered using thin cotton under vacuum. Filtrate was treated with methanol to precipitate agarose dissolved in ILs. Several methanol washings were given to ensure the complete removal of ILs from extracted agarose. Agarose extracted from different experiments were dried under the vacuum and % yield was calculated on the basis of used dried algae weight. After the completion of agarose extraction, ILs were recovered from the methanol-water (filtrate) solutions using rotary evaporator and dried completely under reduced pressure and finally stored into desiccator for further use. Dried recovered ILs were characterized for their purity via analytical techniques (1H and 13C NMR) and were recycled in different extraction experiments. Extraction efficiency of recycled ILs up to four cycles was checked.

2.2.2. Characterization

The 1HN MR and 13C-NMR spectra of native and extracted agarose were recorded on a Bruker Advance-II 200 (Ultra Shield) Spectrometer, Switzerland, at 200 MHz. FTIR spectra of extracted agarose were recorded at room temperature using NICOLET 6700 FTIR spectrometer. Circular dichroism (CD) spectra of agarose solutions (0.05 wt%) in a wavelength range of 180 to 240 nm were recorded on a Jasco J-815 CD spectrometer. Experiments were carried out in a 1 cm path length cuvette at 25˚C, and were expressed as the average of five scans. The response time and the bandwidth were 2 s and 0.2 nm respectively. Samples for recording the spectra were taken in a quartz cuvette which was immediately sealed after sampling to avoid evaporation. The desired temperature was achieved with an inbuilt peltier device. Thermogravimetric analyses were performed on a TGA/ SDTA851 Mettler Toledo under nitrogen atmosphere from 30˚C to 600˚C with a heating rate of 10˚C・min−1. Molecular weight of the extracted agarose was determined through high temperature gel permeation chromatography (HT-GPC) using Waters 2695 separation module equipped with 2414 RI detector and having ultrahydrajel 500 and 120 columns in series. Columns were eluted with 0.1 M aqueous NaNO3 at a flow rate of 0.5 ml/min. Calibration was performed using Dextran standard ranging from 401,000 to 4400 peak molecular weight. The concentration of agarose solutions in water was 0.02 wt%. Melting and gelling temperatures of agarose hydrogels (1.0% w/v) were determined following the method described by Craigie & Leigh [36] . For measurement of the gelling temperature, hot solution of agarose in water was allowed to cool at room temperature while immersing a thermometer in the agarose sol and holding the test tube at an inclined position to have a greater surface area on the top. Gel strength (g∙cm−2) was measured using a Nikkansui type gel tester (Kiya Sei Sakusho

Scheme 1. Schematic of process for the extraction of agarose from red algae Gracilaria dura using ionic liquids (ILs).

Ltd., Tokyo, Japan). Measurements were performed using a solid cylindrical plunger 1.127 cm in diameter. Optical rotation was measured in 0.1 wt% agarose solutions at 30˚C on a Rudolph Digipol 781 Polarimeter (Rudolph Instruments Inc., NJ, USA). Apparent viscosity of 1 wt% agarose solutions was measured on Brookfield viscometer (DV-II + Pro) using SC4-18 spindle at 60 rpm and 80˚C. Sulphate content analysis was performed on Perkin-Elmer ICP-OES optima 2000DV machine following the method described by Wolnik [37] .

3. Results and Discussion

3.1. Agarose Extraction

Imidazolium based ILs having high basicity anions such as [OAc] or [Dep] have been found good dissolution ability for biomass [16] [34] . Such ILs are suitable for biomass processing because having low melting point and viscosity. Besides these, the bio-based IL containing choline as cation counter ion having same anions analogue have also shown the biomass dissolution ability [38] . Therefore, we have chosen three different ILs having high hydrogen bond basicity i.e. [Emim] [OAc], [Ch] [OAc] and [Emim] [Dep] for extraction of agarose from algae (Gracilaria dura) (molecular structure shown in Figure 1) via dissolution process and investigated the effect of nature of cations and anions of ILs on extraction ability towards agarose. Detailed procedural steps carried out for extraction of agarose are summarized in Scheme 1 and Scheme 2. Various parameters such as increase in temperature or microwave heating before normal cooking have been found to assist in rapid and higher dissolution of algae and efficient agarose extraction. Table 1 shows the % yield (calculated on the basis of used dried

Scheme 2. Schematic representation of different steps carried out for efficient extraction of agarose using ionic liquids (ILs).

Table 1. ILs, treatment condition, % yield, gelling temperature (Tgel), melting temperature (Tm), gel strength, apparent viscosity, ash and sulfate content for and standard agarose.

algae weight) of extracted agarose under applied different conditions of heating or combination of microwave heating followed by normal heating. Nearly 2.5 and 7.6 wt% of agarose could be extracted using [Ch] [OAc] and [Emim] [Dep] respectively by simple heating at 80˚C for 2 h, whereas the yield was dramatically higher (28.5 wt%) for [Emim] [OAc] under the similar conditions. It was also observed that by increasing reaction temperature from 80˚C to 100˚C, % yield for agarose extraction increased from 2.5% to 7.5% in [Ch] [OAc], 7.6% to 17% in [Emim] [Dep] and 28.5% to 35% in [Emim] [OAc]. Application of microwave irradiation for 2 min at 5 s pulse prior to the conventional heating at 80˚C increased % yield of agarose in all the ILs. Under coupled conditions of microwave and normal heating, the agarose yield enhanced from 2.5% to 12.5% in [Ch] [OAc], 7.6% to 18.5% in [Emim] [Dep] and 28.5% to 39% in [Emim] [OAc]. The extraction efficiency of used ILs followed the order: [Emim] [OAc] > [Emim] [Dep] > [Ch] [OAc] suggesting that the extraction ability depends on both viscosity and nature of cation/anion of ILs. It is well known that strong hydrogen bonding basicity is effective weakening the hydrogen bonding network of polymer chain, which conclude that higher the β value higher is the dissolution and extraction efficiency [33] [39] . Higher extraction efficiency of imidazolium based ILs containing OAc and Dep anions is due to their high solvating ability, hydrogen bond basicity (β) and low viscosity, while the low extraction in [Ch] [OAc] may be due to the nature of cation of the IL.

3.2. Physicochemical Property of Extracted Agarose and Hydrogels

Structural confirmation and purity of extracted agarose was confirmed by analytical testing as well as by comparison of physicochemical properties with a reference agarose [35] . 1H and 13C NMR spectra of extracted and reference agarose performed in DMSO-d6 solvent at 70˚C are compared in Figure 2 and Figure 3. Reference agarose shows peaks in 1H NMR at δ = C1'-5.21, C1-5.07 and for agarose skeleton (C2-C6) and (C2'-C6')- 4.84-3.8 ppm, while 13C NMR shows peaks at δ = C1'-102.85, C1-98.09, agarose skeleton (C2-C6) and (C2'- C5')-81.68-68.62, and C6'-61.29 ppm. 1H NMR and 13C NMR spectra of extracted agarose show the peaks at the values similar to that of reference agarose confirming its high purity. A comparison of chemical shift values (13C NMR) of extracted agarose in a representative IL, [Emim] [OAc] with the literature is shown in Table 2.

FTIR spectra of the extracted and reference agarose are compared in Figure 4. The FTIR spectra show that extracted agarose match with original structure. Characteristic peaks of agarose from IR bands at 773, 894, and 932 cm−1 because of 3,6-anydro-β-galactose skeletal and 1158 and 1071 cm−1 corresponding to -C-O-C- and glycosidic linkage respectively are present in the extracted agarose [40] -[43] . Circular dichroism (CD) spectra are recorded for examination of conformations in extracted agarose and is compared with confirmations of reference agarose shown in Figure 5. CD spectrum of agarose indicate the overall secondary structure with a positive band centered at ~185 nm [44] . In solution, low intensity of band is the consequence of highly extended chain geometries. Analysis of CD spectra (Figure 5) reveals distinct conformational preferences in the chains of extracted agarose similar to that observed for reference agarose. Extracted material was also checked for thermal stability by performing thermo gravimetric analysis (TGA) and the profiles are shown in Figure 6. The decomposition of extracted and reference agarose is characterized by a narrow temperature range from 275˚C to 300˚C with same onset decomposition temperature (Tdec). No significant weight loss was observed in TGA curves of agarose extracted using [Emim] [OAc] and [Emim] [Dep] before the decomposition temperature suggesting that the extracted agarose had less moisture as compared to reference agarose, whereas in case of agarose extracted using [Ch] [OAc], the moisture content is nearly same to that of reference agarose. Molecular weight of agarose extracted using different ILs was determined from GPC. Molecular weight, polydispersity index and degree of

Figure 2. 1H NMR spectrum of (a) reference agarose and agarose extracted using different ionic liquids (b) [Emim] [OAc], (c) [Ch] [OAc], and (d) [Emim] [Dep].

Figure 3. 13C NMR spectrum of (a) reference agarose and agarose extracted using different ionic liquids (b) [Emim] [OAc], (c) [Ch] [OAc], and (d) [Emim] [Dep]..

Figure 4. Comparison of FTIR spectrum of (a) reference agarose and agarose extracted using different ionic liquids (b) [Emim] [OAc], (c) [Ch] [OAc], and (d) [Emim] [Dep].

Figure 5. Comparison of CD spectra of (a) reference agarose and agarose extracted using different ionic liquids (b) [Emim] [OAc], (c) [Ch] [OAc], and (d) [Emim] [Dep].

Figure 6. Comparison of thermograms for (a) reference agarose and agarose extracted using different ionic liquids (b) [Emim] [OAc], (c) [Ch] [OAc], and (d) [Emim] [Dep].

Table 2. 13C NMR chemical shift comparison of reference and extracted agarose.

G―1,3-β-D-galactose; A―1,4-α-L-3-6-anhydrogalactose.

polymerization of the extracted material are noted in Table 3. Results show that extracted agarose have Mw ranging from 115 to 118 KDa and polydispersity from 2.55 to 2.65 depending upon the IL used. Ash and sulfate content present in the extracted agarose are given in Table 1 and are comparable to that of 3% NaOH treated reference agarose [35] .

Physiochemical property of extracted agarose hydrogels are compared with reference agarose hydrogel in Table 1. Gelling and melting temperature of extracted agarose hydrogels (1 wt%) are slightly lower as compared to the reference agarose, whereas the gel strength is also somewhat lower (~570 to 600 g∙cm−2) as compared to the 3% NaOH treated reference hydrogel (700 g∙cm−2) [35] . This may be due to comparatively higher sulphate content present in extracted agarose. Optical rotation and apparent viscosity of extracted agarose ranged between −22 to −24 and 3.6 to 4.5 cP respectively depending upon the IL used and the values are noted in Table 1 and Table 3.

3.3. % Recycling and Extraction Ability of Recovered ILs

Recycling of used solvents from the reaction mixture after materials extraction is important for the development of an energy-saving process. After the extraction of agarose, as shown in Scheme 1, the filtrates containing a mixture of ILs, methanol and water were evaporated under reduced pressure using rotary evaporator to get pure ILs. Recovered ILs were vacuum dried at 80˚C and were characterized for purity via 1H and 13C NMR (Figure 7). Recycling efficiency and extracting ability was checked for a representative IL, [Emim] [OAc]. Extraction reactions were performed at 80˚C; 2 h for up to four cycles and results of extraction are illustrated in Table 4. Result shows that the extracting of agarose was reduced from 28.5% to 21% after fourth cycle indicating a reduction of about 27% efficiency of extraction. % Recovery of ILs decreased from 87.5% to 46.23% in the fourth cycle which is mainly because of experimental and handling error after each experiment as well as rigidness of the process.

4. Conclusion

In this work, algae (Gracilaria dura) were successfully dissolved in the ILs; [Emim] [OAc], [Ch] [OAc] and

Figure 7. Comparison of NMR spectrum of (a) pure and (b) recycled ionic liquids (ILs) after agarose extraction.

Table 3. Molecular weight (Mn and Mw in kDa), polydispersity (P), degree of polymerization, decomposition temperature (Tdec) and apparent viscosity (cP) of agarose extracted using different ILs.

Table 4. Extraction ability and recovery of 1-ethyl 3-methyl imidazolium acetate in different cycles.

[Emim] [Dep] and high purity agarose could be extracted from algae-IL solutions through precipitation using methanol. In particular, [Emim] [OAc] was found highly efficient for the extraction of agarose. It was observed that the increase in temperature or assistance of microwave heating increased extraction efficiency of agarose from algae. As high as 39 wt% of agarose could be extracted, which is very high as compared to the agarose extracted from conventional methods. ILs could be easily recovered and recycled after the completion of extraction process. Extracted agarose were fully characterized in terms of structural purity and physicochemical properties. The method of extraction developed herein utilizing the ILs is economic and environmental friendly, and looking at the abundance of marine resources, will be highly useful for extraction of value added byproducts.

Acknowledgements

Authors are thankful to Dr. Ramavtar Meena for providing algae samples collected from west coastal regions of India. We thank the centralized instrumental facility of our institute for assistance. Authors are thankful to CSIR, Govt. of India for financial support through the project under chemical science cluster (CSC0203).

NOTES

*Corresponding author.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Schuerch, C. (1986) Polysaccharides in Encyclopedia of Polymer Science and Engineering. 2nd Edition, John Wiley & Sons, New York, 87-162.
[2] Gellerstedt, G., Hon, D.N.-S., Shiraishi, N. and Dekker, M. (2001) Pulping Chemistry in Wood and Cellulosic Chemistry. Marcel Dekker, New York, 859-905.
[3] Huddleston, J.G., Willauer, H.D., Swatloski, R.P., Visser, A.E. and Rogers, R.D. (1998) Room Temperature Ionic Liquids as Novel Media for “Clean” Liquid—Liquid Extraction. Chemical Communication, 135-136.
[4] Rogers, R.D., Seddon, K.R. and Volkov, S. (2002) Green Industrial Applications of Ionic Liquids. NATO Science Series, Kluwer, Dordre CH.
[5] Swatloski, R.P., Spear, S.K., Holbrey, J.D. and Rogers, R.D. (2002) Dissolution of Cellulose with Ionic Liquids. Journal of American Chemical Society, 124, 4974-4975.
http://dx.doi.org/10.1021/ja025790m
[6] Brandt, A., Hallett, J.P., Leak, D.J., Murphy, R.J. and Welton, T. (2010) The Effect of the Ionic Liquid Anion in the Pretreatment of Pine Wood Chips. Green Chemistry, 12, 672-679.
http://dx.doi.org/10.1039/b918787a
[7] Wang, H., Gurau, G. and Rogers, R.D. (2012) Ionic Liquid Processing of Cellulose. Chemical Society Review, 41, 1519-1537.
http://dx.doi.org/10.1039/c2cs15311d
[8] Da Costa Lopes, A.M., João, K.G., C Morais, A.R., Bogel-Lukasik, E. and Bogel-Lukasik, R. (2013) Ionic Liquids as Tool for Lignocellulosic Biomass Fractionation. Sustainable Chemical Processes, 13, 1-31.
http://dx.doi.org/10.1186/2043-7129-1-3
[9] Manic, M.S., Visak, V.N., Ponte, M.N.D. and Visak, Z.P. (2011) Extraction of Free Fatty Acids from Soybean Oil Using Ionic Liquids or Poly(ethyleneglycol)s. AIChE Journals, 57, 1377-1355.
http://dx.doi.org/10.1002/aic.12349
[10] Usuki, T., Yasuda, N., Fujita, M.Y. and Rikukawa, M. (2011) Extraction and Isolation of Shikimic Acid from Ginkgo biloba Leaves Utilizing an Ionic Liquid That Dissolves Cellulose. Chemical Communication, 47, 10560-10562.
http://dx.doi.org/10.1039/c1cc13306c
[11] Ressmann, A.K., Gaertner, P. and Bica, K. (2011) From Plant to Drug: Ionic Liquids for the Reactive Dissolution of Biomass. Green Chemistry, 13, 1442-1447.
http://dx.doi.org/10.1039/c1gc15058h
[12] Chi, Y.S., Zhang, Z.D., Li, C.P., Liu, Q.S., Yan, P.F. and Urs, W.-B. (2011) Microwave-Assisted Extraction of Lactones from Ligusticum chuanxiong Hort. Using Protic Ionic Liquids. Green Chemistry, 13, 666-670.
http://dx.doi.org/10.1039/c0gc00864h
[13] Chowdhury, S.A., Vijayaraghavan, R. and MacFarlane, D.R. (2010) Distillable Ionic Liquid Extraction of Tannins from Plant Materials. Green Chemistry, 12, 1023-1028.
http://dx.doi.org/10.1039/b923248f
[14] Cláudio, A.F.M., Ferreira, A.M., Freire, M.G. and Coutinho, J.A.P. (2013) Enhanced Extraction of Caffeine from Guaraná Seeds Using Aqueous Solutions of Ionic Liquids. Green Chemistry, 15, 2002-2010.
http://dx.doi.org/10.1039/c3gc40437d
[15] Fort, D.A., Remsing, R.C., Swatloski, R.P., Moyna, P., Moyna, G. and Rogers, R.D. (2007) Can Ionic Liquids Dissolve Wood? Processing and Analysis of Lignocellulosic Materials with 1-n-Butyl-3-Methylimidazolium Chloride. Green Chemistry, 9, 63-69.
http://dx.doi.org/10.1039/b607614a
[16] Sun, N., Rahman, M., Qin, Y., Maxim, M.L., Rodrìgues, H. and Rogers, R.D. (2009) Complete Dissolution and Partial Delignification of Wood in the Ionic Liquid 1-Ethyl-3-Methylimidazolium Acetate. Green Chemistry, 11, 646-655.
http://dx.doi.org/10.1039/b822702k
[17] Wang, X., Li, H., Cao, Y. and Tang, Q. (2011) Cellulose Extraction from Wood Chip in an Ionic Liquid 1-Allyl-3- Methylimidazolium Chloride (AmimCl). Bioresource Technology, 102, 7959-7965.
http://dx.doi.org/10.1016/j.biortech.2011.05.064
[18] Hamada, Y., Yoshida, K., Asai, R.-I., Hayase, S., Nokami, T., Izumib, S. and Itoh, T. (2013) A Possible Means of Realizing a Sacrifice-Free Three Component Separation of Lignocellulose from Wood Biomass Using an Amino Acid Ionic Liquid. Green Chemistry, 15, 1863-1868.
http://dx.doi.org/10.1039/c3gc40445e
[19] Lan, W., Liu, C.-F. and Sun, R.-C. (2011) Fractionation of Bagasse into Cellulose, Hemicelluloses, and Lignin with Ionic Liquid Treatment Followed by Alkaline Extraction. Journal of Agriculture and Food Chemistry, 59, 8691-8701.
http://dx.doi.org/10.1021/jf201508g
[20] Tan, S.S.Y., MacFarlane, D.R., Upfal, J., Edye, L.A., Doherty, W.O.S., Patti, A.F., Pringle, J.M. and Scott, J.L. (2009) Extraction of Lignin from Lignocellulose at Atmospheric Pressure Using Alkylbenzenesulfonate Ionic Liquid. Green Chemistry, 11, 339-345.
http://dx.doi.org/10.1039/b815310h
[21] Hossain, M.M. and Aldous, L. (2012) Ionic Liquids for Lignin Processing: Dissolution, Isolation and Conversion. Australian Journal of Chemistry, 65, 1465-1477.
http://dx.doi.org/10.1071/CH12324
[22] Xin, Q., Pfeiffer, K., Prausnitz, J.M., Clark, D.S. and Blanch, H.W. (2012) Extraction of Lignins from Aqueous-Ionic Liquid Mixtures by Organic Solvents. Biotechnology and Bioengineering, 109, 346-352.
http://dx.doi.org/10.1002/bit.24337
[23] Pinkert, A., Goeke D.F., Marsh, K.N. and Pang, S. (2011) Extracting Wood Lignin without Dissolving or Degrading Cellulose: Investigations on the Use of Food Additive-Derived Ionic Liquids. Green Chemistry, 13, 3124-3136.
http://dx.doi.org/10.1039/c1gc15671c
[24] Prado, R., Erdocia, X. and Labidi, J. (2013) Lignin Extraction and Purification with Ionic Liquids. Journal of Chemical Technology and Biotechnology, 88, 1248-1257.
http://dx.doi.org/10.1002/jctb.3965
[25] Meng, L., Kang, S., Zhang, X., Wu, Y. and Sun, R. (2012) Comparative Characterization of Lignins Extracted from Cotton Stalk Based on Complete Dissolution in Different Systems. Industrial & Engineering Chemistry Research, 51, 9858-9866.
http://dx.doi.org/10.1021/ie301118e
[26] Wang, Y.-X. and Cao, X.-J. (2012) Extracting Keratin from Chicken Feathers by Using a Hydrophobic Ionic Liquid. Process Biochemistry, 47, 896-899.
http://dx.doi.org/10.1016/j.procbio.2012.02.013
[27] Huang, G.L., Shi, J., Zhang, K. and Huang, X.L. (2012) Application of Ionic Liquids in the Microwave Assisted Extraction of Pectin from Lemon Peels. Journal of Analytical Methods in Chemistry, 2012, Article ID: 302059.
http://dx.doi.org/10.1155/2012/302059
[28] Qin, Y., Lu, X., Sun, N. and Rogers, R.D. (2010) Dissolution or Extraction of Crustacean Shells Using Ionic Liquids to Obtain High Molecular Weight Purified Chitin and Direct Production of Chitin Films and Fibers. Green Chemistry, 12, 968-971.
http://dx.doi.org/10.1039/c003583a
[29] Barber, P.S., Griggs, C.S., Bonner, J.R. and Rogers, R.D. (2013) Electrospinning of Chitin Nanofibers Directly from an Ionic Liquid Extract of Shrimp Shells. Green Chemistry, 15, 601-607.
http://dx.doi.org/10.1039/c2gc36582k
[30] Ferreira, R., Garcia, H., Sousa, A.F., Petkovic, M., Lamosa, P., Freire, C.S.R., Silvestre, A.J.D., Rebelo, L.P.N. and Pereira, C.S. (2012) Suberin Isolation from Cork Using Ionic Liquids: Characterisation of Ensuing Products. New Journal of Chemistry, 36, 2014-2024.
http://dx.doi.org/10.1039/c2nj40433h
[31] Teixeira, R.E. (2012) Energy-Efficient Extraction of Fuel and Chemical Feedstocks from Algae. Green Chemistry, 14, 419-427.
http://dx.doi.org/10.1039/c2gc16225c
[32] Kim, Y.-H., Choi, Y.-K., Park, J., Lee, S., Yang, Y.-H., Kim, H.J., Park, T.-J., Kim, Y.H. and Lee, S.H. (2012) Ionic Liquid-Mediated Extraction of Lipids from Algal Biomass. Bioresource Technology, 109, 312-315.
http://dx.doi.org/10.1016/j.biortech.2011.04.064
[33] Abe, M., Fukaya, Y. and Ohno, H. (2010) Extraction of Polysaccharides from Bran with Phosphonate or Phosphinate Derived Ionic Liquids under Short Mixing Time and Low Temperature. Green Chemistry, 12, 1274-1280.
http://dx.doi.org/10.1039/c003976d
[34] Fujita, K., Kobayanshi, D., Nakamura, N. and Ohno, H. (2013) Direct Dissolution of Wet and Saliferous Marine Microalgae by Polar Ionic Liquids without Heating. Enzyme Microbial Technology, 52, 199-202.
http://dx.doi.org/10.1016/j.enzmictec.2012.12.004
[35] Meena, R., Siddhanta, A.K., Prasad, K., Ramavat, B.K., Eswaran, K., Thiruppathi, S., Ganesan, M., Mantri, V.A. and Rao, P.V.S. (2007) Preparation, Characterization and Benchmarking of Agarose from Gracilaria dura of Indian Waters. Carbohydrate Polymers, 69, 179-188.
http://dx.doi.org/10.1016/j.carbpol.2006.09.020
[36] Craigie, J.S. and Leigh, C. (1978) Carrageenans and Agars. In: Hellebust, J.A. and Craigie, J.S., Eds., Handbook of Phycological Methods, Cambridge University Press, Cambridge, 109-131.
[37] Wolnik, K.A. (1988) Inductively Coupled Plasma-Emission Spectrometry. Methods Enzymology, 158, 190-205.
http://dx.doi.org/10.1016/0076-6879(88)58056-4
[38] Muhammad, N., Man, Z., Bustam, M.A., Mutalib, M.I.A., Wilfred, C.D. and Rafiq, S. (2011) Dissolution and Delignification of Bamboo Biomass Using Amino Acid-Based Ionic Liquid. Applied Biochemistry and Biotechnology, 165, 998-1009. http://dx.doi.org/10.1007/s12010-011-9315-y
[39] Brandt, A., Gräsvik, J., Hallett, J.P. and Welton, T. (2013) Deconstruction of Lignocellulosic Biomass with Ionic Liquids. Green Chemistry, 15, 550-583.
http://dx.doi.org/10.1039/c2gc36364j
[40] Christiaen, D. and Bodard, M. (1983) Spectroscopieinfrarouge de films d’agar de Gracilariaverrucosa (Huds) Papenfuss. Botanica Marina, 26, 425-427.
[41] Singh, T., Trivedi, T.J. and Kumar, A. (2010) Dissolution, Regeneration and Ion-Gel Formation of Agarose in Room-Temperature Ionic Liquids. Green Chemistry, 12, 1029-1035.
http://dx.doi.org/10.1039/b927589d
[42] Trivedi, T.J., Srivastava, D.N., Rogers, R.D. and Kumar, A. (2012) Agarose Processing in Protic and Mixed Protic- Aprotic Ionic Liquids: Dissolution, Regeneration and High Conductivity, High Strength Ionogels. Green Chemistry, 14, 2831-2839.
http://dx.doi.org/10.1039/c2gc35906e
[43] Trivedi, T.J., Rao, S.K. and Kumar, A. (2014) Facile Preparation of Agarose-Chitosan Hybrid Materials and Nanocomposite Ionogels Using an Ionic Liquid via Dissolution, Regeneration and Sol-Gel Transition. Green Chemistry, 16, 320-330.
http://dx.doi.org/10.1039/c3gc41317a
[44] Rees, D.A. (1981) Polysaccharide Shapes and Their Interactions—Some Recent Advances. Pure & Applied Chemistry, 53, 1-14.
http://dx.doi.org/10.1351/pac198153010001

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.