Changes in the organization of isozyme loci Me1 and Adh1 in-duced with triton X-100 in common wheat lines

Karina Kh. Makhmudova, Nataliya A. Vinichenko, Elizaveta D. Bogdanova, Svetlana S. Kirikovich, Evgenii V. Levites

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DOI: 10.4236/abb.2011.23020                

Abstract

The study of PCR-profiles of isozyme loci Me1 and Adh1 in common wheat lines obtained by means of treatment of initial cultivar Alem with detergent Tri-ton X-100 was carried out by the modified ISSR-amplification method. It was demonstrated that exposure to Triton X-100 causes changes of PCR-profiles of enzyme loci. The obtained data are suggestive of the role of chromatin and nuclear mem-brane interaction in structural-functional genome organization.

Keywords

Common Wheat; Triton X-100; DNA; ISSR-Amplification; Isozymes

Conflicts of Interest

The authors declare no conflicts of interest.

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