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Disinfection Kinetics and Contribution of Reactive Oxygen Species When Eliminating Bacteria with TiO2 Induced Photocatalysis

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DOI: 10.4236/jbnb.2014.53024    3,223 Downloads   3,860 Views   Citations

ABSTRACT

Titania (TiO2) induced photocatalysis has been widely investigated and applied as a disinfection strategy in many industrial and clinical applications. Reactive oxygen species (ROS), including hydroxyl radicals (&8226OH), superoxide radicals () and hydrogen peroxide (H2O2), generated in the photocatalytic reaction process are considered to be the active components prompting the bactericidal effect. In the present work, the kinetics of photocatalytic inactivation of Staphylococcus epidermidis and specific contributions of OH, and H2O2 to the bactericidal process were studied using two disinfection settings sutilizing photocatalytic resin-TiO2 nanocomposite surfaces and suspended TiO2 nanoparticles, respectively. In antibacterial tests against S. epidermidis with a layer of bacterial suspension on the resin-TiO2 surfaces, H2O2 was found to be the most efficient ROS component contributing to the antibacterial effect. Disinfection kinetics showed a two-step behavior with an initial region having a lower disinfection rate followed by a higher rate region after 10 min of UV irradiation. By contrast, in antibacterial tests with suspended bacteria and photocatalytic TiO2 nanoparticles, OH and H2O2 showed equal significance in the bacterial inactivation having a typical Chick-Watson disinfection kinetics behavior with a steady disinfection rate. The results contribute to the understanding of the bactericidal mechanism and kinetics of photocatalytic disinfection that are essential for designing specific antibacterial applications of photocatalytic materials.

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Cai, Y. , Strømme, M. and Welch, K. (2014) Disinfection Kinetics and Contribution of Reactive Oxygen Species When Eliminating Bacteria with TiO2 Induced Photocatalysis. Journal of Biomaterials and Nanobiotechnology, 5, 200-209. doi: 10.4236/jbnb.2014.53024.

References

[1] Matsunaga, T., Tomoda, R., Nakajima, T. and Wake, H. (1985) Photoelectrochemical Sterilization of Microbial-Cells by Semiconductor Powders. FEMS Microbiology Letters, 29, 211-214.
http://dx.doi.org/10.1111/j.1574-6968.1985.tb00864.x
[2] Liou, J.W. and Chang, H.H. (2012) Bacteri-cidal Effects and Mechanisms of Visible Light-Responsive Titanium Dioxide Photocatalysts on Pathogenic Bacteria. Archivum Immunologiae Et Therapiae Experimentalis, 60, 267-275.
http://dx.doi.org/10.1007/s00005-012-0178-x
[3] Lilja, M., Welch, K., Åstrand, M., Engqvist, H. and Strømme, M. (2012) Effect of Deposition Parameters on the Photocatalytic Activity and Bioactivity of TiO2 Thin Films Deposited by Vacuum Arc on Ti-6Al-4V Substrates. Journal of Biomedical Materials Research Part B-Applied Biomaterials, 100B, 1078-1085.
http://dx.doi.org/10.1002/jbm.b.32674
[4] Foster, H.A., Ditta, I.B., Varghese, S. and Steele, A. (2011) Photocatalytic Disinfection Using Titanium Dioxide: Spectrum and Mechanism of Antimicrobial Activity. Applied Microbiology and Biotechnology, 90, 1847-1868.
http://dx.doi.org/10.1007/s00253-011-3213-7
[5] Lilja, M., Forsgren, J., Welch, K., Åstrand, M., Engqvist, H. and Strømme, M. (2012) Photocatalytic and Antimicrobial Properties of Surgical Implant Coatings of Titanium Dioxide Deposited though Cathodic Arc Evaporation. Biotechnology Letters, 34, 2299-2305.
http://dx.doi.org/10.1007/s10529-012-1040-2
[6] Dalrymple, O.K., Stefanakos, E., Trotz, M.A. and Goswami, D.Y. (2010) A Review of the Mechanisms and Modeling of Photocatalytic Disinfection. Applied Catalysis B-Environmental, 98, 27-38.
http://dx.doi.org/10.1016/j.apcatb.2010.05.001
[7] Wu, X.Z., Lingyue, M. and Akiyama, K. (2005) Chemiluminescence Study of Active Oxygen Species Produced by TiO2 Photocatalytic Reaction. Luminescence, 20, 36-40.
http://dx.doi.org/10.1002/bio.800
[8] Cermenati, L., Pichat, P., Guillard, C. and Albini, A. (1997) Probing the TiO2 Photocatalytic Mechanisms in Water Purification by Use of Quinoline, Photo-fenton Generated OH Radicals and Superoxide Dismutase. Journal of Physical Chemistry B, 101, 2650-2658.
http://dx.doi.org/10.1021/jp962700p
[9] Okuda, M., Tsuruta, T. and Katayama, K. (2009) Lifetime and Diffusion Coefficient of Active Oxygen Species Generated in TiO2 Sol Solutions. Physical Chemistry Chemical Physics, 11, 2287-2292.
http://dx.doi.org/10.1039/b817695g
[10] Nosaka, Y., Nakamura, M. and Hirakawa, T. (2002) Behavior of Superoxide Radicals Formed on TiO2 Powder Photocatalysts Studied by a Chemiluminescent Probe Method.Physical Chemistry Chemical Physics, 4, 1088-1092.
http://dx.doi.org/10.1039/b108441k
[11] Popham, P.L. and Novacky, A. (1991) Use of Dimethyl-Sulfoxide to Detect Hydroxyl Radical during Bacteria-Induced Hypersensitive Reaction. Plant Physiology, 96, 1157-1160.
http://dx.doi.org/10.1104/pp.96.4.1157
[12] Pezzuto, J.M. and Park, E.J. (2007) Autoxidation and Antioxidants. In: Swarbrick, J., Ed., Encyclopedia of Phamaceutical Technology, 3rd Edition, Informa Healthcare, New York, 139-154.
[13] Nakano, M., Sugioka, K., Ushijima, Y. and Goto, T. (1986) Chemiluminescence Probe with Cypridina Luciferin Analog, 2-Methyl-6-Phenyl-3,7-Dihydroimidazo[1,2-a]Pyrazin-3-One, for Estimating the Ability of Human-Granulocytes to Generate . Analytical Biochemistry, 159, 363-369.
http://dx.doi.org/10.1016/0003-2697(86)90354-4
[14] Sunada, K., Watanabe, T. and Hashimoto, K. (2003) Studies on Photokilling of Bacteria on TiO2 Thin Film. Journal of Photochemistry and Photobiology A-Chemistry, 156, 227-233.
http://dx.doi.org/10.1016/S1010-6030(02)00434-3
[15] Hirakawa, K., Mori, M., Yoshida, M., Oikawa, S. and Kawanishi, S. (2004) Photo-Irradiated Titanium Dioxide Catalyzes Site Specific DNA Damage via Generation of Hydrogen Peroxide. Free Radical Research, 38, 439-447.
http://dx.doi.org/10.1080/1071576042000206487
[16] Maness, P.C., Smolinski, S., Blake, D.M., Huang, Z., Wolfrum, E.J. and Jacoby, W.A. (1999) Bactericidal Activity of Photocatalytic TiO2 Reaction: Toward an Understanding of Its Killing Mechanism. Applied and Environmental Microbiology, 65, 4094-4098.
[17] Kiwi, J. and Nadtochenko, V. (2004) New Evidence for TiO2 Photocatalysis during Bilayer Lipid Peroxidation. Journal of Physical Chemistry B, 108, 17675-17684.
http://dx.doi.org/10.1021/jp048281a
[18] Cho, M., Chung, H., Choi, W. and Yoon, J. (2004) Linear Correlation between Inactivation of E. coli and OH Radical Concentration in TiO2 Photocatalytic Disinfection. Water Research, 38, 1069-1077.
http://dx.doi.org/10.1016/j.watres.2003.10.029
[19] Wu, P.G., Imlay, J.A. and Shang, J.K. (2010) Mechanism of Escherichia coli Inactivation on Palladium-Modified Nitrogen-Doped Titanium Dioxide.Biomaterials, 31, 7526-7533.
http://dx.doi.org/10.1016/j.biomaterials.2010.06.032
[20] Cho, M. and Yoon, J. (2008) Measurement of OH Radical CT for Inactivating Cryptosporidium Parvum Using Photo/Ferrioxalate and Photo/TiO2 Systems. Journal of Applied Microbiology, 104, 759-766.
http://dx.doi.org/10.1111/j.1365-2672.2007.03682.x
[21] Kikuchi, Y., Sunada, K., Iyoda, T., Hashimoto, K. and Fujishima, A. (1997) Photocatalytic Bactericidal Effect of TiO2 Thin Films: Dynamic View of the Active Oxygen Species Responsible for the Effect. Journal of Photochemistry and Photobiology A-Chemistry, 106, 51-56.
[22] Welch, K., Cai, Y.L., Engqvist, H. and Strømme, M. (2010) Dental Adhesives with Bioactive and On-Demand Bactericidal Properties .Dental Materials, 26, 491-499.
http://dx.doi.org/10.1016/j.dental.2010.01.008
[23] Cai, Y., Strømme, M. and Welch, K. (2013) Photocatalytic Antibacterial Effects Are Maintained on Resin-Based TiO2 Nanocomposites after Cessation of UV Irradiation. PLoS One, 8, Article ID: e75929.
http://dx.doi.org/10.1371/journal.pone.0075929
[24] Welch, K., Cai, Y. and Strømme, M. (2012) A Method for Quantitative Determination of Biofilm Viability. Journal of Functional Biomaterials, 3, 418-431.
http://dx.doi.org/10.3390/jfb3020418
[25] Watson, H.E. (1908) A Note on the Variation of the Rate of Disinfection with Change in the Concentration of the Disinfectant. Journal of Hygiene, 8, 536-542.
http://dx.doi.org/10.1017/S0022172400015928

  
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