OJVM> Vol.4 No.7, July 2014

Bioassay Procedure for the Diagnosis of Aflatoxicosis in a Pig Farm in Nsukka, South East Nigeria

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ABSTRACT

The owner of an intensively reared pig farm in Nsukka, South-East Nigeria reported the deaths (within one week) of 90 piglets (2-3 months of age) out of 150 piglets on the farm. The piglets were being fed home-compounded ration composed of spent grain (which appeared moldy) and other locally sourced materials. Clinical signs observed in affected piglets include sudden loss of appetite, diarrhea and distress grunting sound prior to death. Symptomatic treatment of the piglets which included the use of antibiotics (tetracycline, LA) did not appear to have ameliorated the condition. At necropsy carcasses were generally in fair to good body condition; with subcutaneous hemorrhages, mainly under the skin of head and neck regions. Lungs were congested and edematous, with froth along the tracheal and bronchial airways. The liver and spleen were moderately congested, while the mucosae of the gastrointestinal tract (which was free of ingesta) appeared mildly hyperaemic. No pathogenic bacterium was isolated from the heart blood and spleen. Histologic section of the liver showed centrilobular hepatocytes vacoulation and necrosis with hypertrophy of Kupffer cells that were in erythrophagocytosis. There was moderate fibrinous exudation into the interlobular septae. Spleen section showed severe erythrophagocytosis, but mild haemosiderosis. White pulp was either reactive or depopulated. Lungs were severely haemorrhagic with bronchitis and bronchiolitis. A tentative diagnosis of mycotoxicosis was made and the spent grain-compounded ration was fed to different groups of ducklings, with/without arginine and lysine supplementation. On the bases of clinical signs and mortality pattern; gross and histologic changes in the liver of the ducklings, a definitive diagnosis of aflatoxicosis was made. This paper emphasizes the experimental feeding of suspected feeds/feed ingredient to ducklings as a reliable diagnostic model for aflatoxicosis.

Cite this paper

Shoyinka, S. , Chah, K. , Eze, C. , Ezema, W. , Onoja, I. and Umeakuana, P. (2014) Bioassay Procedure for the Diagnosis of Aflatoxicosis in a Pig Farm in Nsukka, South East Nigeria. Open Journal of Veterinary Medicine, 4, 129-133. doi: 10.4236/ojvm.2014.47015.

References

[1] Abramson, D. (2001) Mycotoxins in Swine Feed. Manitoba Feed Conference Alltech Inc., Nicholasville.
[2] D’Mello, J.P.F. (2000) Anti-Nutritional Factors and Mycotoxins. Farm Animal Metabolism and Nutrition, 383.
[3] Whitlow, L.W., Hagler, W.M. (JR) and Diaz, D.E. (2010) Mycotoxins in Feeds. Foodstuffs Magazine, 74, 74-84. http://fdsmagissues.feedstuffs.com/fds/Reference_issue_2010/13_Mycotoxins%20in%20Fee-
ds.pdf
[4] Bhat, R., Ravishankar, V.R. and Karim, A.A. (2010) Mycotoxins in Food and Feed: Present Status and Future Concern. Comprehensive Reviews in Food Science and Food Safety, 9, 57-81.
[5] Crenshaw, M. (2008) Mycotoxin in Swine Diets.
[6] WHO (2011) Additional Studies on Aflatoxin and Health: Studies on Aflatoxin’s Impact on Health.
[7] Gavin, L.M. (2003) Mycotoxin (Aflatoxin) in Clinical Veterinary Toxicology. In: Konnie, H.P., Ed., Mosby, Inc., 231-235.
[8] Swamy, H.V.L.N. (2009) Mycotoxin Mystery in Pigs: What Are the Causes? Alltech Inc., Nicholasville.
[9] Marin, D.E., Taranu, I., Bunaciu, R.P., Pascale, F., Tudor, D.S., Avram, N., Sarca, M., Cureu, I., Criste, R.D., Suta, V. and Oswald, I.P. (2002) Changes in Performance, Blood Parameters, Humoral and Cellular Immune Responses in Weanling Piglets Exposed to Low Doses of Aflatoxin. Journal of Animal Science, 80, 1250-1257.
[10] Murphy, J.M. and Kane, T.M. (2006) Thinking Globally, Acting Locally. Proceedings of the 6th London Swine Conference.
[11] Hannes, V (2008) Mycotoxins—Overview and Effects in Pigs. AFGRI Animal Feeds.
[12] Cullen, M.J. (2007) Hepatotoxic Agents: Liver, Biliary System, and Exocrine Pancreas. In: Pathologic Basis of Vetrinary Disease, McGavin, M. D. and Zachary, J.F., Eds., 4th Edition, Mosby Elsevier, Berlin, 445.
[13] Gelven, R. (2010) Approaches for Alleviating Toxic Effects of Aflatoxin in Lactating Dairy Cows and Weanling Pigs. A Thesis Presented to the Faculty of the Graduate School at the University of Missouri-Columbia, Columbia, 1-93.
[14] Harvey, R.B., Edrington, T.S., et al. (1995) Influence of Aflatoxin and Fumonisin B1 Containing Culture Material on Growing Barrows. American Journal of Veterinary Research, 56, 1668-1672.
[15] Okoye, J.O.A., Asuzu, I.U. and Gugnani, H.C. (1988) Paralysis and Lameness Associated with Aflatoxicosis in Broilers. Avian Pathology, 17, 731-734.
http://dx.doi.org/10.1080/03079458808436490
[16] Kramer, J.W. (1969) Clinical Enzymology. In: Kaneko, J.J., Ed., Clinical Biochemistry of Domestic Animals, 4th Edition, Academic Press, San Diego,
[17] Madhavan, T.V. and Gopalan, C. (1965) Effect of Dietary Protein on Aflatoxin Liver Injury in Weanling Rats. Archives of Pathology, 80, 123.
[18] Ehrensperger, F. (2007) Pathology of the Spleen; Balgrade. Vetsuisse Faculty, Institute of Veterinary Pathology, 1-16.
[19] Newberne, P.M., Gerald, N.W. and Allen, III, H. (1966) Effects of Dietary Modifications on Response of the Duckling to Aflatoxin. The Journal of Nutrition, 90, 123-130.
[20] Austwick, P.K.C. (1978) Aflatoxicosis in Poultry: In: Wyllie, T.D. and Morehouse, L.G., Eds., Mycotoxic Fungi, Mycotoxins, Mycotoxicosis; an encyclopedic handbook, Vol. 2, Mycotoxicosis of Domestic and Laboratory Animals, Poultry and Aquatic Invertebrates and Vertebrates, Mercel Dekker, New York.

  
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