The Application of Nutrimetabolomics to Investigating the Bioaccessibility of Nutrients in Ham Using a Batch in Vitro Digestion Model

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DOI: 10.4236/fns.2014.51003    3,160 Downloads   4,671 Views   Citations

ABSTRACT

Delivering high quality dietary protein at an affordable price is a major aim of the EU-funded CHANCE project. Foods have been formulated with this aim and as part of their nutritional assessment; the bioaccessbility of nutrients following simulated gastroduodenal digestion is being investigated. Nutrimetabolomics approaches can be used to comprehensively and quantitatively analyse nutrients and metabolites. They have been applied to monitor nutrient release from ham, formulated in the CHANCE project, during in vitro digestion. SDS-PAGE analysis shows that constituent ham proteins were broken down to lower molecular weight polypeptides (Mr 10 kDa) after 120 min simulated gastric digestion which was digested further by subsequent duodenal digestion. Digestion of porteins resulted in the appearance of coalesced lipid droplets associated with the loss of the muscle protein matrix of the ham. Important nutrients, such as choline, creatine, carnosine, sucrose, cholesterol, triacylglyceride and fatty acids (saturated and unsaturated) were identified using 1H NMR. Chance ham is a good source of dietary protein and the combined approach can provide representative data on the bioaccessibility of all detectable nutrients contained in CHANCE ham to human digestive system.

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Pan, X. , Smith, F. , Cliff, M. , Capozzi, F. and Mills, E. (2014) The Application of Nutrimetabolomics to Investigating the Bioaccessibility of Nutrients in Ham Using a Batch in Vitro Digestion Model. Food and Nutrition Sciences, 5, 17-26. doi: 10.4236/fns.2014.51003.

References

[1] S. J. Hur, et al., “In Vitro Human Digestion Models for Food Applications,” Food Chemistry, Vol. 125, No. 1, 2011, pp. 1-12.
http://dx.doi.org/10.1016/j.foodchem.2010.08.036
[2] K. A. Kopf-Bolanz, et al., “Validation of an in Vitro Digestive System for Studying Macronutrient Decomposition in Humans,” Journal of Nutrition, Vol. 142, No. 2, 2012, pp. 245-250.
http://dx.doi.org/10.3945/jn.111.148635
[3] S. J. Hur, et al., “The Effects of Biopolymer Encapsulation on Total Lipids and Cholesterol in Egg Yolk during in Vitro Human Digestion,” International Journal of Molecular Sciences, Vol. 14, No. 8, 2013, pp. 16333-16347.
http://dx.doi.org/10.3390/ijms140816333
[4] S. J. Hur, et al., “Effects of Various Fiber Additions on Lipid Digestion during in Vitro Digestion of Beef Patties,” Journal of Food Science, Vol. 74, No. 9, 2009, pp. C653-C657.
http://dx.doi.org/10.1111/j.1750-3841.2009.01344.x
[5] D. G. Fatouros and A. Mullertz, “In Vitro Lipid Digestion Models in Design of Drug Delivery Systems for Enhancing Oral Bioavailability,” Expert Opinion on Drug Metabolism & Toxicology, Vol. 4, No. 1, 2008, pp. 65-76.
http://dx.doi.org/10.1517/17425255.4.1.65
[6] M. Wickham, R. Faulks and C. Mills, “In Vitro Digestion Methods for Assessing the Effect of Food Structure on Allergen Breakdown,” Molecular Nutrition & Food Research, Vol. 53, No. 8, 2009, pp. 952-958.
http://dx.doi.org/10.1002/mnfr.200800193
[7] C. H. M. Versantvoort, et al., “Applicability of an in Vitro Digestion Model in Assessing the Bioaccessibility of Mycotoxins from Food,” Food and Chemical Toxicology, Vol. 43, No. 1, 2005, pp. 31-40.
http://dx.doi.org/10.1016/j.fct.2004.08.007
[8] L. Marciani, et al., “Antral Motility Measurements by Magnetic Resonance Imaging,” Neurogastroenterology and Motility, Vol. 13, No. 5, 2001, pp. 511-518.
http://dx.doi.org/10.1046/j.1365-2982.2001.00285.x
[9] L. Marciani, et al., “Monitoring of Gallbladder and Gastric Coordination by EPI,” Journal of Magnetic Resonance Imaging, Vol. 21, No. 1, 2005, pp. 82-85.
http://dx.doi.org/10.1002/jmri.20223
[10] S. Ballance, et al., “Evaluation of Gastric Processing and Duodenal Digestion of Starch in Six Cereal Meals on the Associated Glycaemic Response Using an Adult Fasted Dynamic Gastric Model,” European Journal of Nutrition, Vol. 52, No. 2, 2013, pp. 799-812.
http://dx.doi.org/10.1007/s00394-012-0386-5
[11] D. A. Volpe, “Drug-Permeability and Transporter Assays in Caco-2 and MDCK Cell Lines,” Future Medicinal Chemistry, Vol. 3, No. 16, 2011, pp. 2063-2077.
http://dx.doi.org/10.4155/fmc.11.149
[12] K. Dettmer, P. A. Aronov and B. D. Hammock, “Mass Spectrometry-Based Metabolomics,” Mass Spectrometry Reviews, Vol. 26, No, 1, 2007, pp. 51-78.
http://dx.doi.org/10.1002/mas.20108
[13] F. Dieterle, et al., “NMR and MS Methods for Metabonomics,” Methods in Molecular Biology, Vol. 691, 2011, pp. 385-415.
http://dx.doi.org/10.1007/978-1-60761-849-2_24
[14] J. H. Winnike, et al., “Effects of a Prolonged Standardized Diet on Normalizing the Human Metabolome,” The American Journal of Clinical Nutrition, Vol. 90, No. 6, 2009, pp. 1496-1501.
http://dx.doi.org/10.3945/ajcn.2009.28234
[15] G. Pratico, et al., “Exploring Human Breast Milk Composition by NMR-Based Metabolomics,” Natural Product Research, 2013.
http://dx.doi.org/10.1080/14786419.2013.843180
[16] C. Fotakis, et al., “NMR Metabolite Profiling of Greek Grape Marc Spirits,” Food Chemistry, Vol. 138, No. 2-3, 2013, pp. 1837-1846.
http://dx.doi.org/10.1016/j.foodchem.2012.11.128
[17] A. Bordoni, et al., “NMR Comparison of in Vitro Digestion of Parmigiano Reggiano Cheese Aged 15 and 30 Months,” Magnetic Resonance in Chemistry, Vol. 49, Suppl. 1, 2011, pp. S61-S70.
http://dx.doi.org/10.1002/mrc.2847
[18] J. M. Hakumaki and R. A. Kauppinen, “1H NMR Visible Lipids in the Life and Death of Cells,” Trends in Biochemical Sciences, Vol. 25, No. 8, 2000, pp. 357-362.
http://dx.doi.org/10.1016/S0968-0004(00)01614-5
[19] G. Mandalari, et al., “In Vitro Digestibility of Beta-Casein and Beta-Lactoglobulin under Simulated Human Gastric and Duodenal Conditions: A Multi-Laboratory Evaluation,” Regulatory Toxicology and Pharmacology, Vol. 55, No. 3, 2009, pp. 372-381.
http://dx.doi.org/10.1016/j.yrtph.2009.08.010
[20] F. J. Moreno, et al., “Stability of the Major Allergen Brazil Nut 2S Albumin (Ber e 1) to Physiologically Relevant in Vitro Gastrointestinal Digestion,” FEBS Journal, Vol. 272, No. 2, 2005, pp. 341-352.
http://dx.doi.org/10.1111/j.1742-4658.2004.04472.x
[21] H. Wu, et al., “High-Throughput Tissue Extraction Protocol for NMRand MS-Based Metabolomics,” Analytical Biochemistry, Vol. 372, No. 2, 2008, pp. 204-212.
http://dx.doi.org/10.1016/j.ab.2007.10.002
[22] I. K. Straadt, M. D. Aaslyng and H. C. Bertram, “Assessment of Meat Quality by NMR—An Investigation of Pork Products Originating from Different Breeds,” Magnetic Resonance in Chemistry, Vol. 49, Suppl. 1, 2011, pp. S71-S78. http://dx.doi.org/10.1002/mrc.2805
[23] V. Tugnoli, et al., “1H-NMR and 13C-NMR Lipid Profiles of Human Renal Tissues,” Biopolymers, Vol. 72, No. 2, 2003, pp. 86-95. http://dx.doi.org/10.1002/bip.10299
[24] V. Govindaraju, K. Young and A. A. Maudsley, “Proton NMR Chemical Shifts and Coupling Constants for Brain Metabolites,” NMR in Biomedicine, Vol. 13, No. 3, 2000. pp. 129-153.
http://dx.doi.org/10.1002/1099-1492(200005)13:3<129::AID-NBM619>3.0.CO;2-V
[25] M. Gottschalk, et al., “Metabolomic Studies of Human Lung Carcinoma Cell Lines Using in Vitro 1H NMR of Whole Cells and Cellular Extracts,” NMR in Biomedicine, Vol. 21, No. 8, 2008, pp. 809-819.
http://dx.doi.org/10.1002/nbm.1258
[26] D. S. Wishart, et al., “HMDB 3.0—The Human Metabolome Database in 2013,” Nucleic Acids Research, Vol. 41, 2013, pp. D801-D807.
http://dx.doi.org/10.1093/nar/gks1065
[27] J. H. Choe, et al., “The Relation between Glycogen, Lactate Content and Muscle Fiber Type Composition, and Their Influence on Postmortem Glycolytic Rate and Pork Quality,” Meat Science, Vol. 80, No. 2, 2008, pp. 355362. http://dx.doi.org/10.1016/j.meatsci.2007.12.019
[28] D. B. Silk, G. K. Grimble and R. G. Rees, “Protein Digestion and Amino Acid and Peptide Absorption,” Proceedings of the Nutrition Society, Vol. 44, No. 1, 1985, pp. 63-72.
http://dx.doi.org/10.1079/PNS19850011
[29] S. Chu, and M. L. Schubert, :Gastric Secretion,” Current Opinion in Gastroenterology, Vol. 29, No. 6, 2013, pp. 636-641. http://dx.doi.org/10.1097/MOG.0b013e328365efc7
[30] A. M. Wang, Ma, C., Z. H. Xie and F. Shen, “Use of Carnosine as a Natural Anti-Senescence Drug for Human Beings,” Biochemistry (Mosc), Vol. 65, No. 7, 2000, pp. 869-871.
[31] C. Bauchart, et al., “Carnosine Concentration of Ingested Meat Affects Carnosine Net Release into the Portal Vein of Minipigs,” The Journal of Nutrition, Vol. 137, No. 3, 2007, pp. 589-593.
[32] J. K. Blusztajn, “Choline, a Vital Amine,” Science, Vol. 281, No. 5378, 1998, pp. 794-795.
http://dx.doi.org/10.1126/science.281.5378.794
[33] B. Banerjee, et al., “Effect of Creatine Monohydrate in Improving Cellular Energetics and Muscle Strength in Ambulatory Duchenne Muscular Dystrophy Patients: A Randomized, Placebo-Controlled 31P MRS Study,” Magnetic Resonance Imaging, Vol. 28, No. 5, 2010, pp. 698707. http://dx.doi.org/10.1016/j.mri.2010.03.008
[34] R. W. Purchas, J. R. Busboom and B. H. P. Wilkinson, “Changes in the Forms of Iron and in Concentrations of Taurine, Carnosine, Coenzyme Q10, and Creatine in Beef Longissimus Muscle with Cooking and Simulated Stomach and Duodenal Digestion,” Meat Science, Vol. 74, No. 3, 2006, pp. 443-449.
http://dx.doi.org/10.1016/j.meatsci.2006.03.015
[35] J. M. Hakumaki and R. A. Kauppinen, “1H NMR Visible Lipids in the Life and Death of Cells,” Trends in Biochemical Sciences, Vol. 25, No. 8, 2000, pp. 357-362.
http://dx.doi.org/10.1016/S0968-0004(00)01614-5
[36] X. Pan, et al., “The Size of Cytoplasmic Lipid Droplets Varies between Tumour Cell Lines of the Nervous System: a 1H NMR Spectroscopy Study,” Magnetic Resonance Materials in Physics, Biology and Medicine, Vol. 25, No. 6, 2012, pp. 479-485.
http://dx.doi.org/10.1007/s10334-012-0315-x

  
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