Biopolymer Sponge for High Grade Renal Trauma: An Experimental Study in Rabbits

Abstract

Introduction: Acute blunt or open renal trauma sometimes requires conservative measure which involves the use of specific materials in order to adequately restore renal parenchima. The purpose of this study is to analyze the effects of the sugar cane biopolymer sponge in an experimental model of renal trauma in rabbits. Methods: Experimental prospective study was paired with twelve New Zealand rabbits. After induction of renal trauma bilateral high-grade (grade 4-collecting system, according to AAST), the sugar cane biopolymer sponge was used for homeostasis and reconstruction of the right renal parenchyma (experimental group). The same procedure was performed in the left kidney, but using the Surgifoam? sponge (Control Group). The animals were sacrificed after four weeks. Histological analysis was performed and the results were compared after statistically being evaluated. Results: Comparing the experimental group with the control, there was more inflammatory reaction and premature adhesions in the first one. Concerning the influence on efficacy and biocompatibility of the biopolymer, no serious complications like massive bleeding, haematoma, fistula or urinary stone formation were found in both groups. Conclusion: The sugar cane biopolymer sponge demonstrated good efficacy in relation to hemostatic control and reconstruction of the renal parenchyma without major complications. Future studies may direct its routine use in urologic practice.

Share and Cite:

L. Tavares, F. Vilar, J. Aguiar, A. Paz, F. Melo and G. Negromonte, "Biopolymer Sponge for High Grade Renal Trauma: An Experimental Study in Rabbits," Open Journal of Urology, Vol. 4 No. 1, 2014, pp. 1-6. doi: 10.4236/oju.2014.41001.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] M. Paterson-Beedle, J. F. Kennedy, et al., “A Cellulosic Exopolysaccharide Produced from Sugarcane Molasses by a Zoogloea sp.,” Carbohydrate Polymers, Vol. 42, No. 4, 2000, pp. 375-383.
[2] L. W. Jonathan, B. N. Avery, et al., “Renal and Extrarenal Predictors of Nephrectomy from the National Trauma Data Bank,” Journal of Urology, Vol. 175, No. 3, 2006, pp. 970-975.
[3] J. Peters, J. Green, et al., “Kidney Salvage Using the Fibrinogen-and Thrombin-Coated Sponge TachoSil® during Nephron-Sparing Surgery for the Resection of Large Renal Tumors,” Annals of The Royal College of Surgeons of England, Vol. 90, No. 5, 2008, pp. 1-3.
[4] X. M. D. Roa Saveedra, J. A. Cajigas Plata, et al., “Sellantes de Fibrin en Urología,” Urológica Colombiana, Vol. 17, No. 1, 2008, pp. 55-62.
[5] K. Hwang, S. G. Kim and D. J. Kim, “Hypoglossal-Facial Nerve Anastomosis in the Rabbits Using Laser Welding,” Annals of Plastic Surgery, Vol. 61, No. 4, 2008, pp. 452456.
[6] N. Y. Oct & Gul, A. Topal, I. T. Cangul and K. Yanik, “The Effects of Topical Tripeptide Copper Complex and Helium-Neon Laser on Wound Healing in Rabbits,” Expert Review of Dermatology, Vol. 19, No. 1, 2008 pp. 7-14.
[7] K. Bjorses and J. Holst, “Topical Haemostatics in Renal Trauma—An Evaluation of Four Different Substances in an Experimental Setting,” Journal of Trauma, Vol. 66, No. 3, 2009, pp. 602-1
[8] R. Dimitrov, D. Kostov, et al., “Anatomotopographical and Morphological Analysis of Normal Kidneys of Rabbit (Oryctolagus cuniculus),” Trakia Journal of Sciences, Vol. 10, No. 2, 2012, pp. 79-84.
[9] F. Massole and V. Anesthesiology, “Pharmacology and technology,” 4th Edition, Guanabara Koogan, Rio de Janeiro, 2003.
[10] A. J. Wein, L. R. Kavoussi, et al., “Campbell’s Urology,” 7 Edition, WB Saunders, Philadephia, 1998.
[11] E. J. Hick, A. F. Morey, et al., “Gelatin Matrix Treatment of Complex Renal Injuries in a Porcine Model,” Urology Journal, Vol. 173, No. 5, 2005, pp. 1801-1804.
[12] C. M. M. B. Castro, J. L. A. Aguiar, et al., “Cytotoxicity Biopolymer Sugar Cane,” Anais da Faculdadede Medicina da Universidade Federal de Pernambuco, Vol. 49, No. 2, 2004, pp. 119-123.
[13] F. Vilar, J. L. Aguiar, et al., “Peyonie Disease: Study of a New Material with a View to Clinical Application,” International Brazilian Journal of Urology, Vol. 32, No. 2, 2006, p. 167.
[14] S. V. Lima, J. L. A. Aguiar, et al., “A New Dressing for Hypospadias Surgery,” International Brazilian Journal of Urology, Vol. 32, No. 2, 2006, p. 189.
[15] E. M. Lins, “Sugar Cane Biopolymer Membrane as a Patch in Femoral Artery in Dogs,” PhD Thesis, Federal University of Pernambuco, Recife, 2007.
[16] S. Lima, A. Rangel, et al., “A New Bulking Agent to Treat Vesicoureteral Reflux: An Experimental Study,” Journal of Urology, Vol. 185, No. 1, 2011, pp. 106-108.
[17] K. Bjorses and J. Holst, “Topical Haemostatics in Renal Trauma—An Evaluation of Four Different Substances in an Experimental Setting,” Journal of Trauma, Vol. 66, No. 3, 2009, pp. 602-611.
[18] R. W. Hutchinson, D. Broughton, et al., “Hemostatic Effectiveness of Fibrin Pad after Partial Nephrectomy in Swine,” Journal of Surgical Research, Vol. 167, No. 2, 2011, pp. 291-298.
[19] K. H. Attar, J. Namasivavam, et al., “Kidney Salvage using the Fibrinogen and Thrombin Coated Sponge TachoSil during Nephron-Sparing Surgery for the Resection of Large Renal Tumors,” Annals of The Royal College of Surgeons of England, Vol. 90, No. 5, 2008, pp. 1-4.
[20] T. B. Pedersen, J. L. Honge, et al., “Comparative Study of Lung Sealants in a Porcine ex Vivo Model,” The Annals of Thoracic Surgery, Vol. 94, No. 1, 2012, pp. 234-240.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.