Anti-diabetic and antioxidant effects of virgin coconut oil in alloxan induced diabetic male Sprague Dawley rats

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DOI: 10.4236/jdm.2013.34034    3,532 Downloads   6,115 Views   Citations

ABSTRACT

Oxidative stress has been discovered to be involved in the progression of diabetes mellitus. The antioxidant properties of virgin coconut oil (VCO) among other functions might have a beneficial effect in ameliorating the disease. This study was aimed to determine the glycemic and antioxidant effects of VCO in alloxan induced diabetic rats. 24 male Sprague-Dawley rats were divided into 4 groups as follows: control (C), diabetes untreated (DUT), diabetes treated with 7.5 ml/kg VCO (DT7.5) and diabetes treated with 10 ml/kg VCO (DT10). Alloxan (100 mg/kg b.w I.P) was used to induce diabetes and VCO was administered orally once daily for 4 weeks. Fasting blood glucose level was measured on Day 0 (72 hours post alloxan injection) and after 4 weeks. Glucose tolerance test was conducted on the 4th week as well as the determination of serum insulin and liver antioxidant parameters using standard biochemical methods. Values are means ± S.E.M., compared by ANOVA and Tukey’s post hoc test. The results show that VCO significantly reduced the fasting blood glucose level in DT7.5 rats (132.4 ± 6.911) and DT10 rats (131.6 ± 12.2) are compared with DUT rats (320.4 ± 22.99) and improved the oral glucose tolerance. Serum insulin was increased in DT10 rats. GSH activities significantly increased p < 0.05 in DT10 rats (0.39 ± 0.022) when compared to DUT rats (0.032 ± 0.004). CAT activities also significantly increased p < 0.05 in DT7.5 (17.63 ± 0.61) and DT10 rats (30.88 ± 0.97) when compared to DUT rats (10.98 ± 0.6). SOD activities significantly increased p < 0.05 in DT7.5 (2.634 ± 0.04) and DT10 rats (2.258 ± 0.32) when compared to DUT rats (1.366 ± 0.05) while MDA significantly reduced p < 0.05 in DT7.5 (49.16 ± 0.51) and DT10 (33.64 ± 0.42) rats when compared to DUT rats (99.93 ± 4.79). This study revealed that VCO has a hypoglycemic action, enhances insulin secretion and also ameliorates oxidative stress induced in type I (alloxan-induced diabetic) male rats.

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Iranloye, B. , Oludare, G. and Olubiyi, M. (2013) Anti-diabetic and antioxidant effects of virgin coconut oil in alloxan induced diabetic male Sprague Dawley rats. Journal of Diabetes Mellitus, 3, 221-226. doi: 10.4236/jdm.2013.34034.

References

[1] American Diabetes Association (2010) Diagnosis and classification of diabetes mellitus. Diabetic Care, 33.
[2] World Health Organization (2011) Diabetes fact sheet. Sheet number 312 August.
[3] Lenzen, S. (2008) The mechanisms of alloxanand streptozotocin-induced Diabetes. Diabetologia, 51, 216-226. http://dx.doi.org/10.1007/s00125-007-0886-7
[4] Nevin, K.G. and Rajamohan, T. (2006) Virgin coconut oil supplemented diet increases the antioxidant status in rats. Food Chemistry, 99, 260-266. http://dx.doi.org/10.1016/j.foodchem.2005.06.056
[5] Dosumu, O.O., Duru, F.I.O., Osinubi, A.A., Oremosu, A.A. and Noronha, C.C. (2010) Influence of virgin coconut oil (VCNO) on oxidative stress, serum testosterone and gonadotropic hormones (FSH, LH) in chronic ethanol ingestion. Agriculture and Biology Journal of North America, 1, 1126-1132.
[6] Van Immerseel, F., De Buck, J. and Boyen, F. (2004) Medium-chain fatty acids decrease colonization and invasion through hilA suppression shortly after infection of chickens with Salmonella enterica serovar enteritidis. Applied and Environmental Microbiology, 70, 3582-3587. http://dx.doi.org/ 10.1128/AEM.70.6.3582-3587.2004
[7] Takeuchi, H., Sekine, S., Kojima, K. and Aoyama, T. (2008) The application of medium-chain fatty acids: Edible oil with a suppressing effect on body fat accumulation. Asia Pacific Journal of Clinical Nutrition, 17, 320-324.
[8] Anosike, C.A. and Obidoa, O. (2010) Anti-inflammatory and anti-ulcerogenic effect of ethanol extract of coconut (Cocos nucifera) on experimental rats. African Journal of Food, Agriculture, Nutrition and Development, 10, 10-16.
[9] Cretney, J. and Tafunai, A. (2004) Tradition, trade, and technology: Virgin coconut oil in samoa. In Chains of Fortune: Linking Women Producers and Workers with Global Markets, 45-74.
[10] Uchiyama, M. and Mihara, M. (1978) Determination of malonaldehyde precursor in tissues by thiobarbituris acid test. Analytical Biochemistry, 86, 271-278. http://dx.doi.org/10.1016/0003-2697(78)90342-1
[11] Sun, M. and Zigmam S. (1978) An improved spectrophotomeric assay for superoxide dismutase based on epinephrine autooxidation. Analytical Biochemistry, 90, 81-89. http://dx.doi.org/10.1016/ 0003-2697(78)90010-6
[12] Aebi, H. (1984) Catalase in vitro. Methods in Enzymology, 8, 121-126. http://dx.doi.org/10.1016/ S0076-6879(84)05016-3
[13] Van Dooran, R., Liejdekker, C.M. and Handerson, P.T. (1978) Synergistic effects of phorone on the hepatotoxicity of bromobenzene and paracetamol in mice. Toxicology, 11, 225-233. http://dx.doi.org/ 10.1016/S0300-483X(78)91389-6
[14] Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254. http://dx.doi.org/10.1016/0003-2697(76)90527-3
[15] Szkudelski, T. (2001) The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological Research, 50, 536-546.
[16] Lenzen, S., Tiedge, M., Jorns, A. and Munday, R. (1996) Alloxan derivatives as a tool for the elucidation of the mechanism of the diabetogenic action of alloxan. In: Shafrir, E., Ed., Lessons from Animal Diabetes. Birkhauser, Boston, 113-122. http://dx.doi.org/10.1007/978-1-4612-4112-6_8
[17] Jorns, A., Munday, R., Tiedge, M. and Lenzen, S. (1997) Comparative toxicity of alloxan, N-alkylalloxans and ninhydrin to isolated pancreatic islets in vitro. Journal of Endocrinology, 155, 283-293. http://dx.doi.org/10.1677/joe.0.1550283
[18] Garfinkel, M., Lee, S., Opara, E.C. and Akwari, O.E. (1992) Insulinotropic potency of lauric acid. A metabolic rationale for medium chain fatty acids (MCF) in TPN formulation. Journal of Surgical Research, 52, 328-333. http://dx.doi.org/10.1016/0022-4804(92)90111-C
[19] Sadikot, S.M. (2005) Coconut for health nutrition. Jakarta, APCC, 6.
[20] Kim, S.S., Gallaher, D.D. and Csallany, A.S. (2000) Vitamin E and probucol reduce urinary lipophilic aldehydes and renal enlargement in streptozotocin induced diabetic rats. Lipids, 35, 1225-1237. http://dx.doi.org/10.1007/s11745-000-0639-2
[21] Siddalingaswamy, M., Rayaorth, A. and Khanum, F. (2011) Anti-diabetic effects of cold and hot extracted virgin coconut oil. Journal of Diabetes Mellitus, 1, 118-123. http://dx.doi.org/10.4236/ jdm.2011.14016
[22] Maritim, A.C., Sanders, R.A. and Watkins, J.B. (2003) Diabetes, oxidative stress and antioxidants: A review. Journal of Biochemical and Molecular Toxicology, 17, 24-38. http://dx.doi.org/10.1002/ jbt.10058
[23] Mekinova, D., Chorvathova, V., Volkovova, K., Staruchova, M., Grancicova, F., Klvanova, J., Nevin, K.G. and Rajamohan, T. (2004) Beneficial effects of virgin coconut oil on lipid parameters and in vitro LDL oxidation. Clinical Biochemistry, 37, 830-835. http://dx.doi.org/10.1016/j.clinbiochem.2004.04.010
[24] Borenshtein, D.R., Ofri, M., Werman, A., Stark, H.J., Tritschler, W. and Moeller Madar Z. (2001) Cataract development in diabetic sand rats treated with alpha-lipoic acid and its gamma-linolenic acid conjugate. Diabetes/ Metabolism Research and Reviews, 17, 44-50.
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