Health> Vol.5 No.10A, October 2013

Recombinant protein detection and its content in total protein, lipids and toxic antinutritional substances in Mexican maize

ABSTRACT

The engineering genetic technology has developed Bt maize events which contain recombinant protein that will be safe for the consumer. The aflatoxins are contaminants present in maize capable of producing cancer and decreasing the immune response in human, additionally contained polyphenols compounds considered non nutritive. The objective of this study was to identify the presence of recombinant protein in hybrid and local varieties of corn and evaluate the content of aflatoxins and tannins. 25 samples of white grain maize for human consumption were collected, 12 were for hybrid maize and 13 local varieties, from the states of Hidalgo, Mexico and Morelos. Samples were analyzed for Cry1Ab/Cry1Ac, using lateral flow strip method, crude protein and lipids by standard methods. Aflatoxins were assessed by comercial Elisa kit and tannins by spectroscopy method. The data were grouped in a completely random model and an analysis of variance was performed. The results indicated that 44.5% of hybrid corn was positive by Bt-Cry1Ab/1Ac proteins, containing 9.02% ± 2.5 lipids and 11.33% ± 2.2 crude protein, 189 ± 0.92 mg/g of tannins and 6.36 ± 3.3 μg·g-1 aflatoxins. The local maize samples (55.5%) were negative to Bt-Cry1Ab/ Cry1Ac, which protein content was of 8.68% ± 0.90, 6.14% lipids ± 2.3, 273 ± 0.40 mg/100g tannin and 7.15 ± 3.3 μg·g-1 of aflatoxins. In conclusion, we observed an improvement of nutrient composition in hybrid maize with Bt proteins, and decrease in tannins content comparing with some local varieties without Bt proteins. The effectiveness of Bt maize expressing the Cry1Ab/ Cry1Ac in reducing aflatoxin contamination was not observed, therefore, additive affects of aflatoxins contamination in maize Bt-Cry need to be further investigated in cancer disease development.

KEYWORDS


Cite this paper

Silvia Denise, P. , Eduardo, P. and Benjamín, V. (2013) Recombinant protein detection and its content in total protein, lipids and toxic antinutritional substances in Mexican maize. Health, 5, 9-13. doi: 10.4236/health.2013.510A1002.

References

[1] James, C. (2004) Executive summary: Global status of commercialized biotech/GM crops. ISAAA Briefs No. 32. ISAAA, Ithaca.
[2] Betz, F.S., Hammond, B.G. and Fuchs, R.L. (2000) Safety and advantages of Bacillus thuringiensis-protected plants to control insect pests. Regulatory Toxicology and Pharmacology, 32, 156-173.
http://dx.doi.org/10.1006/rtph.2000.1426
[3] Boisvert, M. and Boisvert, J. (2000) Effects of Bacillus thuringiensis var. israelensis on target and non target organisms. A review of laboratory and field experiments. Biocontrol Science and Technology, 10, 517-561.
http://dx.doi.org/10.1080/095831500750016361
[4] Sevenier, R.M., van der Meer, I., Bino, R. and Koops, A.J. (2002) Increased production of nutriments by genetically engineered crops. Journal of the American College of Nutrition, 21, 199S-204S.
http://dx.doi.org/10.1080/07315724.2002.10719266
[5] Rubens, O.N. (2009) Calidad de los análisis de riesgo e inseguridad de los transgénicos para la salud ambiental y humana. La Revista Peruana de Medicina Experimental y Salud Pública, 26, 74-82.
[6] Traavik, T and Heinemann, J. (2007) Genetic engineering and omitted health research: Still no answers to ageing questions. Third World Network, Penang.
[7] Hails, H. and Kindererer, G. (2003) The GM public debate: Context and communication strategies. Nature Reviews in Genetics, 4, 819-826.
http://dx.doi.org/10.1038/nrg1182
[8] Rance, F. Allergie alimentaire a l′ arachide et aux fruits a coque. Allergologie Pedriatic, Paris.
[9] López, M.L.X., Oliart, R. and Valerio, A.G. (2009) Antioxidant activity, phenolic compounds and anthocyanins content of eighteen strains of Mexican maize. Food Science and Technology, 42, 1187-1192.
[10] Grisolic, C.K., Olibeira, F. E.C., Ramos, F.R., Lopes, M.C., Muniz, F.D.H. and Monnerat, R.G. (2009) Acute toxicity and citotoxicity of Bacillus thuringiensis and Bacillus saphcericus strains in fish and mouse bone narrow. Ecotoxicology, 18, 22-26.
http://dx.doi.org/10.1007/s10646-008-0252-7
[11] Peterson, G, Cunningham, S., Deutsch, L., Erickso, J., Quinla, A. and Raez, L.E. (2000) The risk and benefits of genetically modified crops: a multidisciplinary perspective. Conservation Ecology, 4, 13-18.
[12] Chassy, B.M. (2002) Food safety evaluation of crops produced through biotechnology. Journal of the American College of Nutrition, 21, 166-173.
http://dx.doi.org/10.1080/07315724.2002.10719261
[13] Einspanier, R., Klotz, A., Kraft, J., Aulrich, K., Poser, R., Schwagele, F., Jahreis, G. and Flachowsky, G. (2001) The fate of forage plant DNA in farm animals: A collaborative case-study investigating cattle and chicken fed recombinant plant material. European Food Research and Technology, 212, 129-134.
http://dx.doi.org/10.1007/s002170000248
[14] Nowa, K.W. (2007) New perspectives for use of native and engineered recombinants foods and proteins untreat of food allergy. Immunology and Allergy Clinics of North America, 27, 105-127.
http://dx.doi.org/10.1016/j.iac.2006.11.006
[15] Herrera, E.L. and Simpson, J. (1995) Genetically engineered resistance to bacterial and fungal pathogens. World Journal of Microbiology & Biotechnology, 11, 383-392.
http://dx.doi.org/10.1007/BF00364613
[16] ILSI (1996) Allergy and Immunology Institute and International Food Biotechnology Council. Allergenicity of food produced by genetic modification. CRC Press, Boca Raton.
[17] ILSI (2004) Comprehensive reviews in food science and food safety. Food Science & Technology, 3, 44-49.
[18] Wolfenbarger, L.L. and Phifer, P.R. (2000) The ecological risks and benefits of genetically engineered plants. Science, 290, 2088-2093.
http://dx.doi.org/10.1126/science.290.5499.2088
[19] American Association of Cereal Chemists (2000) Approved methods of the AACC.
[20] Association of Official Analytical Chemists (1999) Official methods of analysis vol II.
[21] Dewanto V., Wu, X. and Liu, R.H. (2002) Processed sweet corn has higher antioxidant activity. Journal of Agricultural and Food Chemistry, 50, 4959-4964.
http://dx.doi.org/10.1021/jf0255937
[22] Kuiper, H.A., Kleter, G.A., Noteborn, H.P., Kok, E.J. (2001) Assessment of the food safety issues related to genetically modified foods. Plant Journal, 27, 503-528.
http://dx.doi.org/10.1046/j.1365-313X.2001.01119.x
[23] Kanny, G., Monret, D., Flabbee, J., Beaudouin, E., Morisset, M. and Thevenin, F. (2001) Population study of food allergy in France. Journal of Allergy and Clinical Immunology, 108, 133-140.
http://dx.doi.org/10.1067/mai.2001.116427
[24] Cesaretto, L., Vascotto C. and Calligaris, S. (2004) The importance of redox state in liver damage. Annals of Hepatology, 3, 86-93.
[25] Lipton, C.R., Dautlick, J.X., Grothaus, G.D., Hunst, P.L., Magin, K.M., Mihaliak, C.A., Rubio, F.M. and Stave, J.W. (2000) Guidelines for the validation and use of immunoassays for determination of introduced proteins in biotechnology enhanced crops and derived food ingredients. Food and Agricultural Immunology, 12, 153-164.
http://dx.doi.org/10.1080/095401000404094
[26] Cockburn, A. (2002) Assuring the safety of genetically modified foods. The importance of an holistic integrative approach. Journal of Biotechnology, 98, 79-106.
http://dx.doi.org/10.1016/S0168-1656(02)00088-3
[27] Zolla, L., Rinalducci, S., Antonioli, P. and Righetti, P.G. (2008) Proteomics as a complementary tool for identifying unintended side effects occurring in transgenic maize seeds as a result of genetic modifications. Journal of Proteome Research, 7, 1850-1861.
http://dx.doi.org/10.1021/pr0705082
[28] Ortiz-García, S., Ezcurra, B., Schoel, F, Acevedo, J. Soberón and Snow, A. (2006) Transgenic maize in Mexico, BioScience, 56, 709-803.
http://dx.doi.org/10.1641/0006-3568(2006)56[709:TMIM]2.0.CO;2
[29] Williams, W.P., Gary, C., Buckley, P.M. and Daves, A.A. (2002) Aflatoxin accumulation in conventional and transgenic corn hybrid infested with southern corn borer. J. Agric. Urban Entomology, 19, 227-236.

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