Methods for Detection of Viruses in Water and Wastewater

Alicja Hryniszyn, Magdalena Skonieczna, Jarosław Wiszniowski

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Environmental Biotechnology Department, Silesian University of Technology, Gliwice, Poland.

DOI: 10.4236/aim.2013.35060                

Abstract

Viruses present in water might be harmful for human health and life. Nowadays over 100 pathogenic human virus species occur in water polluted with sewage. Chlorination, which is the most popular disinfection method is not able to remove easily viruses from treated water. Due to this, it’s necessary to detect viruses in water before treatment in order to determine disinfectant dose and to ensure the sanitary safety level of treated water. The aim of this article is to review viruses detection methods as well as the problems related to implementation of those methods in analysis of water and wastewater samples.

Keywords

Viruses; Analytical Problems; Environmental Samples; Detection Methods

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	U. Desselberger and J. Gray, “Viral Gastroenteritis: Causes, Pathophysiology, Immunology, Treatment and Epidemiology,” In: U. Desselberger and J. Gray, Eds., Viral Gastroenteritis, Elsevier Science B.V., AE Amsterdam, 2003, pp. 1-8.
[2]	G. Virella, “Mikrobiologia i Choroby Zakazne,” 3rd Edition, Wydawnictwo Urban & Partner, Wroclaw, 2000.
[3]	A. Bosh, “Human Enteric Viruses in the Water Environment: A Mini Review,” International Microbiology, Vol. 1, No. 3, 1998, pp. 191-196.
[4]	W. Irving, D. Ala’Aldeen and T. Boswell, “Mikrobiologia Medyczna. Krótkie Wyklady,” Wydawnictwo Naukowe PWN, Warszawa, 2008.
[5]	D. Roy, P. K. Wong, R. S. Engelbrecht and E. S. Chian, “Mechanism of Enteroviral Inactivation by Ozone,” Applied Environmental Microbiology, Vol. 41, No. 3, 1981, pp. 718-723.
[6]	Z. Dymaczewski, J. A. Oleszkiewicz and M. M. Sozański, “Poradnik Eksploatatora Oczyszczalni Scieków,” 2nd Edition, Wydawnictwo PZITS, Poznań, 1997.
[7]	W. Li, X. Wang, C. Q. Yuan, J. L. Zheng, M. Jin, N. Song, et al., “Detection of Enteroviruses and Hepatitis a Virus in Water by Consensus Primer Multiplex RT-PCR,” Word Journal of Gastroenterology, Vol. 8, No. 4, 2002, pp. 699-702.
[8]	S. Pina, M. Pulig, F. Lucena, J. Jofre and R. Girones, “Viral Pollution in the Environment and Shellfish: Human Adenovirus Detection by PCR as an Index of Human Viruses,” Applied and Environmental Microbiology, Vol. 64, No. 9, 1998, pp. 3376-3382.
[9]	S. H. Lee, C. Lee, K. W. Lee, H. B. Cho and S.-J. Kim, “The Simultaneous Detection of Both Enteroviruses and Adenoviruses in Environmental Water Samples Including Tap Water with an Integrated Cell Culture-Multiplex-Nested-PCR Procedure,” Journal of Applied Microbiology, Vol. 98, No. 5, 2005, pp. 1020-1029. doi:10.1111/j.1365-2672.2004.02496.x
[10]	H. B. Cho, S. H. Lee, J. C. Cho and S. J. Kim, “Detection of Adenoviruses and Enteroviruses in Tap Water and River Water by Reverse Transcription Multiplex PCR,” Canadian Journal of Microbiology, Vol. 46, No. 5, 2000, pp. 417-424. doi:10.1139/w00-014
[11]	G. S. Fout, B. C. Martinson, M. W. N. Moyer and D. R. Dahling, “A Multiplex Reverse Transcription-PCR Method for Detection of Human Enteric Viruses in Groundwater,” Applied and Environmental Microbiology, Vol. 69, No. 6, 2003, pp. 3158-3164. doi:10.1128/AEM.69.6.3158-3164.2003
[12]	E. Schvoerer, F. Bonnet, V. Dubois, G. Cazaux, R. Serceau, H. J. A. Fleury, et al., “PCR Detection of Human Enteric Viruses in Bathing Areas, Waste Waters and Human Stools in Southwestern France,” Research in Microbiology, Vol. 151, No. 8, 2000, pp. 693-701. doi:10.1016/S0923-2508(00)90132-3
[13]	S. A. Rutjes, H. H. J. L. van den Berg, W. J. Lodder and A. M. de Roda Husman, “Real-Time Detection of Noroviruses in Surface Water by Use of a Broadly Reactive Nucleic Acid Sequence-Based Amplification Assay,” Applied and Environmental Microbiology, Vol. 72, No. 8, 2006, pp. 5349-5358. doi:10.1128/AEM.00751-06
[14]	J. Jean, B. Blais, A. Darvau and I. Fliss, “Simultaneous Detection and Identification of Hepatitis A Virus and Rotavirus by Multiplex Nucleic Acid Sequence-Based Amplification (NASBA) and Microtiter Plate Hybridization System,” Journal of Virological Methods, Vol. 105, No. 1, 2002, pp. 123-132. doi:10.1016/S0166-0934(02)00096-4
[15]	J. Jean, B. Blais and I. Fliss, “Detection of Hepatitis A Virus by the Nucleic Acid Sequence-Based Amplification Technique and Comparison with Reverse Transcription-PCR,” Applied and Environmental Microbiology, Vol. 67, No. 12, 2001, pp. 5593-5600. doi:10.1128/AEM.67.12.5593-5600.2001
[16]	S. R. Nettikadan, J. C. Johnson, C. Mosher and E. Henderson, “Virus Particle Detection by Solid Phase Immunocapture and Atomic Force Microscopy,” Biochemical and Biophysical Research Communications, Vol. 311, No. 2, 2003, pp. 540-545. doi:10.1016/j.bbrc.2003.10.022
[17]	F. S. Alhamlan, M. M. Ederer, T. L. Green, C. K. Brinkman, E. R. Coats and R. L. Crawford, “A Novel Screening Tool Using Microarray and PCR to Detect Pathogens in Agricultural Impacted Waters,” International Journal of Environmental Protection, Vol. 3, No. 3, 2013, pp. 17-32.
[18]	S. H. Hara, K. Terauchi and I. Koike, “Abundance of Viruses in Marine Waters: Assessment by Epifluorescence and Transmission Electron Microscopy,” Applied and Environmental Microbiology, Vol. 57, No. 9, 1991, pp. 2731-2734.
[19]	K. P. Henneses and C. A. Suttle, “Direct Counts of Viruses in Natural Waters and Laboratory Cultures by Epifluorescence Microscopy,” Limnology and Oceanography, Vol. 40, No. 6, 1995, pp. 1050-1055. doi:10.4319/lo.1995.40.6.1050
[20]	R. T. Noble and J. A. Fuhrman, “Use of SYBR Green I for Rapid Epifluorescence Counts of Marine Viruses and Bacteria,” Aquatic Microbial Ecology, Vol. 14, No. 2, 1998, pp. 113-118. doi:10.3354/ame014113
[21]	A. Shibata, Y. Goto, H. Saito, T. Kikuchi, T. Toda and S. Taguchi, “Comparison of SYBR Green I and SYBR Gold Stains for Enumerating Bacteria and Viruses by Epifluorescence Microscopy,” Aquatic Microbial Ecology, Vol. 43, No. 3, 2006, pp. 221-231.
[22]	K. Wen, A. C. Ortmann and C. A. Suttle, “Accurate Estimation of Viral Abundance by Epifluorescence Microscopy,” Applied and Environmental Microbiology, Vol. 70, No. 7, 2004, pp. 3862-3867. doi:10.1128/AEM.70.7.3862-3867.2004
[23]	M. G. Weinbauer and C. A. Suttle, “Comparison of Epifluorescence and Transmission Electron Microscopy for Counting Viruses in Natural Marine Waters,” Aquatic Microbial Ecology, Vol. 13, No. 3, 1997, pp. 225-232. doi:10.3354/ame013225
[24]	Y. Bettarel, T. Sime-Ngando, C. Amblard and H. Laveran, “A Comparison of Methods for Counting Viruses in Aquatic Systems,” Applied and Environmental Microbiology, Vol. 66, No. 6, 2000, pp. 2283-2289. doi:10.1128/AEM.66.6.2283-2289.2000
[25]	M. C. Alonso, J. Rodríguez and J. J. Borrego, “Enumeration and Isolation of Viral Particles from Oligotrophic Marine Environments by Tangential Flow Filtration,” International Microbiology, Vol. 2, No. 4, 1999, pp. 227-232.
[26]	K. E. Wommack, R. T. Hill, M. Kessel, E. Russek-Cohen and R. R. Colwell, “Distribution of Viruses in the Chesapeake Bay,” Applied and Environmental Microbiology, Vol. 58, No. 9, 1992, pp. 2965-2970.
[27]	W. T. Liu and L. Zhu, “Environmental Microbiology-on-Chip and Its Future Impacts,” Trends in Biotechnology, Vol. 23, No. 4, 2005, pp. 174-178. doi:10.1016/j.tibtech.2005.02.004
[28]	O. V. Rengevych, Y. M. Shirshov, Y. V. Ushenin and G. V. Bektov, “Separate Determination of Thickness and Optical Parameters by Surface Plasmon Resonance: Accuracy Consideration,” Semiconductor Physics, Quantum Electronics & Optoelectronics, Vol. 2, No. 2, 1999, pp. 28-35.
[29]	L. Kittigul, B. Raengsakulrach,S. Siritanikorn, R. Kanyok, P. Diraphat, V. Thirawutch, et al., “Detection of Poliovirus, Hepatitis A Virus and Rotavirus from Sewage and Water Samples,” The Southeast Asian Journal Health of Tropical Medicine and Public Health, Vol. 31, No. 1, 2000, pp. 41-45.
[30]	A. M. Nasser and T. G. Metcalf, “An A-ELISA to Detect Hepatitis A Virus in Estuarine Samples,” Applied and Environmental Microbiology, Vol. 53, No. 5, 1987, pp. 1192-1195.
[31]	N. A. El-Esnaw, “Examination for Hepatitis E Virus in Wastewater Treatment Plants and Workers by Nested RT-PCR and ELISA,” The Journal of the Egyptian Public Health Association, Vol. 75, No. 1-2, 2000, pp. 219-231.
[32]	M. K. Nishida, V. L. A. Ruiz and F. Gregori, “Detection of Rotavirus from Pig Livestock Wastewater of Sao Paulo State, Brazil,” Ars Veterinaria, Vol. 25, No. 3, 2009, pp. 136-141.
[33]	D Marie, C. P. D. Brussard, R. Thyrhaug and G. Bratbak, “Enumeration of Marine Viruses in Culture and Natural Samples by Flow Cytometry,” Applied and Environmental Microbiology, Vol. 65, No. 1, 1999, pp. 45-52.
[34]	F. X. Abad, R. M. Pinto and A. Bosch, “Flow Cytometry Detection of Infectious Rotaviruses in Environmental and Clinical Samples,” Applied and Environmental Microbiology, Vol. 64, No. 7, 1998, pp. 2392-2396.
[35]	C. P. D. Brussaard, “Optimization of Procedures for Counting Viruses by Flow Cytometry,” Applied and Environmental Microbiology, Vol. 70, No. 3, 2004, pp. 1506-1513. doi:10.1128/AEM.70.3.1506-1513.2004
[36]	F. Chen, J. R. Lu, B. J. Binder, Y. C. Liu and R. E. Hodson, “Application of Digital Image Analysis and Flow Cytometry to Enumerate Marine Viruses Stained with SYBR Gold,” Applied and Environmental Microbiology, Vol. 67, No. 2, 2001, pp. 539-545. doi:10.1128/AEM.67.2.539-545.2001
[37]	T. Straub, J. L. Pepper and C. P. Gerba, “Comparison of PCR and Cell Culture for Detection of Enteroviruses in Sludge of Their Transport,” Applied and Environmental Microbiology, Vol. 61, No. 5, 1995, pp. 2066-2068.
[38]	B. B. Haab, M. J. Dunham and P. O. Brown, “Protein Microarray for Highly Parallel Detection and Quantification of Specific Proteins and Antibodies in Complex Solutions,” Genome Biology, Vol. 2, No. 2, 2000, pp. 1-13.
[39]	H. Zhu and M. Snyder, “Protein Arrays and Microarrays,” Current Opinion in Chemical Biology, Vol. 5, No. 1, 2001, pp. 40-45. doi:10.1016/S1367-5931(00)00170-8
[40]	D. Egger, L. Pasamontes, M. Ostermayer and K. Bienz, “Reverse Transcription Multiplex PCR for Differentiation between Polio and Enteroviruses from Clinical and Environmental Samples,” Journal of Clinical Microbiology, Vol. 33, No. 6, 1995, pp. 1442-1447.
[41]	K. A. Reynolds, C. P. Gerba and J. L. Pepper, “Detection of Infectious Enteroviruses by an Integrated Cell Culture-PCR Procedure,” Applied and Environmental Microbiology, Vol. 62, No. 4, 1996, pp. 1424-1427.
[42]	F. L. Guyader, E. Dubois, D. Menard and M. Pommepuy, “Detection of Hepatitis A Virus, Rotavirus and Enterovirus in Naturally Contaminated Shellfish and Sediment by Reverse Transcription-Seminested PCR,” Applied and Environmental Microbiology, Vol. 60, No. 10, 1994, pp. 3665-3671.
[43]	C. Gantzer, S. Senouci, A. Maul, Y. Levi and L. Schwarzbold, “Enteroviruses Genomes in Wastewater: Concentration on Glass Wool and Glass Powder and Detection by RT-PCR,” Journal of Virological Methods, Vol. 65, No. 2, 1997, pp. 265-271. doi:10.1016/S0166-0934(97)02193-9
[44]	J. M. Los, P. Golec, G. Wegrzyn, A. Wegrzyn and M. Los, “Simple Method for Plating Escherichia coli Bacteriophage Forming Very Small Plaques or No Plaques under Standard Conditions,” Applied and Environmental Microbiology, Vol. 74, No. 16, 2008, pp. 5113-5120. doi:10.1128/AEM.00306-08
[45]	J. C. Espinoza and J. Kuznar, “Rapid Simultanous Detection and Quantitation of Infectious Pancreatic Necrosis Virus (IPNV),” Journal of Virological Methods, Vol. 105, No. 1, 2002, pp. 81-85. doi:10.1016/S0166-0934(02)00083-6
[46]	A. Raszka, A. Ziembińska and A. Wiechetek, “Metody i Techniki Biologii Molekularnej w Biotechnologii Srodowiskowej,” Technical Transactions. Environmental Engineering, Vol. 2, No. 2, 2009, pp. 101-114.
[47]	P. M. Lydyard, A. Whelan and M. W. Fanger, “Immunologia. Krókie Wyklady,” 2nd Edition, Wydawnictwo Naukowe PWN, Warszawa, 2006.
[48]	J. M. Brown, D.M. Coates and R. J. Phillpots, “Evaluation of Monoclonal Antibodies for Generic Detection of Flaviviruses by ELISA,” Journal of Virological Methods, Vol. 62, No. 1, 1996, pp. 143-151. doi:10.1016/S0166-0934(96)02095-2
[49]	R. D. Saville, N. T. Constantine, F. R. Cleghorn, N. Jack, C. Bartholomew, J. Edwards, et al., “Fourth-Generation Enzyme-Linked Immunosorbent Assay for Simultanous Detection of Human Immunodeficiency Virus Antigen and Antibody,” Journal of Clinical Microbiology, Vol. 39, 2001, pp. 2518-2524. doi:10.1128/JCM.39.7.2518-2524.2001
[50]	R. Yadav and S. Dwivedi, “Trends and Perspectives of Biosensors for Food and Environmental Virology,” Food and Environmental Virology, Vol. 2, No. 2, 2010, pp. 53-63. doi:10.1007/s12560-010-9034-5
[51]	D. R. Reyes, D. Jossifidis and P. A. Auroux, “Micro Total Analysis Systems. 1. Introduction, Theory and Technology,” Analytical Chemistry, Vol. 74, No. 12, 2002, pp. 2623-2636. doi:10.1021/ac0202435
[52]	Y. L. Tsai, B. Tran, L. R. Sangermano and C. J. Palmer, “Detection of Poliovirus, Hepatitis A Virus, and Rotavirus by Triplex Reverse Transcriptase PCR,” Applied and Environmental Microbiology, Vol. 60, No. 7, 1994, pp. 2400-2700.
[53]	K. R. Everett, J. Rees-George, I. P. S. Puspharajah, B. J. Jansen and Z. Luo, “Advantages and Disadvantages of Microarrays to Study Microbial Population Dynamics,” New Zealand Plant Protection Society Journals, Vol. 63, 2010, pp. 1-6.
[54]	H. B. Balkin and A. B. Margolin, “Detection of Poliovirus by ICC/qPCR in Concentrated Water Samples Has Greater Sensitivity and Is Less Costly Using BGM Cells in Suspension as Compared to Monolayers,” Virology Journal, Vol. 7, No. 282, 2010, pp. 1-4.
[55]	Y. Marzouk and S. M. Goyal, “Prevalence of Enteroviruses in Ground Water of Israel,” Ground Water, Vol. 17, No. 5, 1979, pp. 487-491. doi:10.1111/j.1745-6584.1979.tb03345.x
[56]	K. P. Hennes and C. A. Suttle, “Direct Counts of Viruses in Natural Waters and Laboratory Cultures by Epifluorescence Microscopy,” Limnology and Oceanography, Vol. 40, No. 6, 1995, pp. 1050-1055. doi:10.4319/lo.1995.40.6.1050
[57]	A. Piekarowicz, “Podstawy Wirusologii Molekularnej,” Wydawnictwo Naukowe PWN, Warszawa, 2004.
[58]	S. J. Martin, “The Biochemistry of Viruses,” Cambridge University Press, Cambridge, 1978.
[59]	M. Keusgen, “Biosensors: New Approaches in Drug Discovery,” Naturwissenschaften, Vol. 89, No. 10, 2002, pp. 433-444. doi:10.1007/s00114-002-0358-3