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A Low Cost and Versatile STED Superresolution Fluorescent Microscope

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DOI: 10.4236/mi.2013.23007    4,337 Downloads   8,818 Views   Citations

ABSTRACT

A versatile and inexpensive super-resolution fluorescent microscope that functions as easily as a conventional confocal microscope is described. Components of the microscope were designed on a platform which was placed atop a surplus microscope frame. All optical components and equipments used are given. The excitation and depletion beams are extracted from a compact low-cost supercontinuum light source. The focal spot of the depletion beam at the focal plane is studied and imaged by a 100 nm aperture near-field fiber tip. The collinear excitation and depletion beam focused by a 0.9 numerical aperture microscope objective produce a 90 nm lateral super-resolution as verified by imaging 100 nm diameter fluorescent beads.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

D. McBride, C. Su, J. Kameoka and S. Vitha, "A Low Cost and Versatile STED Superresolution Fluorescent Microscope," Modern Instrumentation, Vol. 2 No. 3, 2013, pp. 41-48. doi: 10.4236/mi.2013.23007.

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