Insecticidal Activity of Bacillus thuringiensis on Larvae and Adults of Bactrocera oleae Gmelin (Diptera:Tephritidae)

Faiza Ilias, Nassira Gaouar, Kanza Medjdoub, Mireille Kallassy Awad

Faculty of Science, Saint- Joseph University, Beirut, Lebanon.
Laboratory of Ecology and Management of Natural Ecosystems, Tlemcen University, Tlemcen, Algeria.

DOI: 10.4236/jep.2013.45056                

Abstract

The olive fruit fly Bactrocera oleae is a major olive pest in Algeriaand other Mediterranean countries. Its attacks, in some cases, seriously compromised production. Bacillus thuringiensis is a spore-forming soil bacterium which produces a protein crystal toxic to some insects. The objective of this study was to search for isolates toxic to larvae and adults of B. oleae. Four doses test were performed on neonate larvae, second instar larvae and third instar larvae. Seven Bt strains examined, showed highest toxicity levels against adults. 86% mortality was observed against neonate larvae after 7 days of application by Bt toxin isolate. The mortality of the second instar larvae was 65% mortality at dose of 10⁸ UFC/mL. The third instar larvae was very susceptible to Bt strain with 70% of mortality. This study demonstrated that some local Algerian B. thuringiensis isolates exhibit toxic potential that could be used to control B. oleae.

Keywords

Bacillus thuringiensis; Bactrocera oleae; Insecticidal Activity; Olive fly; Diptera

Conflicts of Interest

The authors declare no conflicts of interest.

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