Share This Article:

Spectrophotometric determination of some calcium channel blockers using Sulfochlorophenol-S, Bromopyrogallol red, Eriochromecyanine-R and Pyrocatechoil violet

Full-Text HTML Download Download as PDF (Size:979KB) PP. 514-525
DOI: 10.4236/ns.2013.54065    3,125 Downloads   5,150 Views   Citations

ABSTRACT

Four simple, sensitive and accurate spectrophotometric methods have been developed for the determination of some calcium channel blockers: Amlodipine besylate (ADB), Diltiazem hydrochloride (DTZ) and Verapamil hydrochloride (VPM) in pharmaceutical formulations. These methods based on formation ion pair complexes with Sulfochlorophenol-S (SCPS), Bromopyrogallol red (BPR), Eriochromecyanine- R (ECC) and Pyrocatechol violet (PCV) in acidic medium. The colored products are extracted with chloroform and measured spectrophoto- metrically at 462, 600, 440 and 442 for ECC, SCPS, BPR and PCV, respectively. Beer’s low was obeyed in the concentration range, for ECC: 25 - 175 μg·ml-1, 50 - 150 μg·ml-1 or 100 - 250 μg·ml-1, for SCPS: 300 - 800 μg·ml-1, 200 - 700 μg·ml-1 or 100 - 550 μg·ml-1, for BPR: 50 - 400 μg·ml-1, 200 - 700 μg·ml-1 or 200 - 700 μg·ml-1 for VPM, DTZ or ADB, respectively and for PCV: 50 - 250 μg·ml-1 for VPM or 200 - 500 μg·ml-1 for DTZ with molar absorptivity, for ECC: 2.2 × 104, 2.1 × 104, 1.6 × 104 L·mol-1·cm-1, for SCPS: 3.8 × 103, 5.6 × 103, 8.1 × 103 L·mol-1·cm-1, for BPR: 11 × 103, 4.8 × 103, 6.9 × 103 L·mol-1·cm-1 for VPM, DTZ or ADB, respectively and for PCV: 19.5 × 103 L·mol-1·cm-1 for VPM and 6.6 × 103 L·mol-1·cm-1 for DTZ and relative standard deviation, for ECC: 0.76%, 0.86%, 0.46%, for SCPS: 0.94%, 0.96%, 0.86%, for BPR: 0.96%, 0.95%, 0.55% for VPM, DTZ or ADB, respectively and for PCV: 0.81% for VPM and 0.65% for DTZ. These methods have been successfully applied for the assay of drug in pharmaceutical formulations. No interference was observed from common pharmaceutical adjuvants. Statistical comparison of the results with the reference method shows excellent agreement and indicates no significant difference in accuracy and precision.

Cite this paper

Omar, M. , Abdelmageed, O. , Abdelgaber, A. and Saleh, S. (2013) Spectrophotometric determination of some calcium channel blockers using Sulfochlorophenol-S, Bromopyrogallol red, Eriochromecyanine-R and Pyrocatechoil violet. Natural Science, 5, 514-525. doi: 10.4236/ns.2013.54065.

References

[1] Delgado, J.M. and Remers, W.R. (1998) Text book of organic medicinal and pharmaceutical chemistry. 9th Edition, J. B. Lippincott Co., Philadelphia, New York and London, 543, 544, 583.
[2] Mohammadi, A., Rezanour, N., Dogaheh, M.A., Bidkorbeh, F.G., Hashem, M. and Walker, R.B. (2007) A stability-indicating high performance liquid chromatographic (HPLC) assay for the simultaneous determination of atorvastatin and amlodipine in commercial tablets. Journal of Chromatography, B: Analytical Technologies in the Bio medical and Life Sciences, 846, 215-221. doi:10.1016/j.jchromb.2006.09.007
[3] Li, C.P., Yan, X.P. and Shan, W.G. (2006) HPLC determination of amlodipine besylate tablets. Chinese Journal of Pharmaceutical Analysis, 26, 1878-1879.
[4] Bhushan, R., Gupta, D. and Singh, S.K. (2006) Liquid chromatographic separation and UV determination of certain antihypertensive agents. Biomedical Chromatography, 20, 217-224. doi:10.1002/bmc.557
[5] Zendelovska, D., Stafilov, T. and Stefova, M. (2003) De termination of diltiazem in human plasma after solid phase and liquid-liquid extraction. Analytical and Bio analytical Chemistry, 376, 848-853. doi:10.1007/s00216-003-1996-9
[6] Ola, M.A. and Amr, M.B. (2011). Derivative-ratio spectrophotometric, chemometric and HPLC validated methods for simultaneous determination of amlodipine and atorvastatin in combined dosage form. International Journal of Industrial Chemistry, 2, 78-85.
[7] Najma, S., Muhammad, S.A. and Waheed, A. (2011) In-vitro interaction studies of verapamil with fluoroquinolones using first order dervative UV spectrophotometry and RP-HPLC. Journal of the Chilean Chemical Society, 56, 848-855. doi:10.4067/S0717-97072011000400005
[8] Venkatesh, G., Ramanathan, S., Mansor, S.M., Nair, N.K., Sattar, M.A., Croft, S.L. and Navaratnam, V. (2007) Development and validation of RP-HPLC-UV method for simultaneous determination of buparvaquone, atenolol, propranolol, quinidine and verapamil: A tool for the standardization of rat in situ intestinal permeability studies. Journal of Pharmaceutical and Biomedical Analysis, 43, 1546-1551. doi:10.1016/j.jpba.2006.11.013
[9] Jhee, O.H., Hong, J.W., Om, A.S., Lee, M.H., Lee, W.S., Shaw, L.M., Lee, J.W. and Kang J.S. (2005) Direct de termination of verapamil in rat plasma by coupled column microbore-HPLC method. Journal of Pharmaceutical and Biomedical Analysis, 37, 405-410. doi:10.1016/j.jpba.2004.11.003
[10] Bharat, K.D., Jitendra, P., Pranati, C., Shabana, B. and Suddhasatya, D. (2010). Analytical method development and validation of Amlodipine and Benazepril hydrochlo ride in combined dosage form by RP-HPLC. International Journal of Chemical and Pharmaceutical Sciences, 2, 26-30.
[11] Bhushan, R., Gupta, D. and Singh, S.K. (2006) Liquid chromatographic separation and UV determination of certain antihypertensive agents. Biomedical Chromatography, 20, 217-224. doi:10.1002/bmc.557
[12] Aniruddha, R.C., Swati, C.J., Kumbhar, S.V., Vinayak, J.K., Vinit, D.P., Bhanudas, S.K. and Pradeep, D.L. (2010) Simultaneous HPTLC estimation for Telmisartan and Amlodipine besylate in tablet dosage form. Archives of Applied Science Research, 2, 94-100.
[13] Vidhya, K.B. and Sunil, R.D. (2011) Validated HPTLC method for simultaneous estimation of atenolol, hydro chlorothiazide and amlodipine besylate in bulk drug and formulation. International Journal of Analytical and Bio analytical Chemistry, 1, 70-76.
[14] Deval, B.P., Falgun, A.M. and Kashyap, K.B. (2012) Simultaneous estimation of amlodipine besylate and in dapamide in pharmaceutical formulation by thin-layer chromatographic-densitometric method. Novel Science International Journal of Pharmaceutical Science, 1, 74-82.
[15] Kowalczuk, D. (2005) Simultaneous high-performance thin-layer chromatography densitometric assay of tran dolapril and verapamil in the combination preparation. Journal of AOAC International, 88, 1525-1529.
[16] Bhushan, R. and Gupta, D. (2005) Thin-layer chromatography separation of enantiomers of verapamil using macrocyclic antibiotic as a chiral selector. Biomedical Chromatography, 19, 474-478. doi:10.1002/bmc.520
[17] Scharpf, F., Riedel, K.D., Laufen, H. and Leitold, M. (1994) Enantioselective gas chromatographic assay with electron-capture detection for amlodipine in biological samples. Journal of Chromatography B: Biomedical Sciences and Applications, 655, 225-233. doi:10.1016/0378-4347(94)00068-9
[18] Shin, H.S., Oh-Shin, Y.S., Kim, H.J. and Kang, Y.K. (1996) Journal of Chromatography B: Biomedical Sciences and Applications, 677, 369-373.
[19] Kalin, J.R., Wood, K.M. and Lee, A.J. (1994) Journal of Analytical Toxicology, 18, 180-182.
[20] Alebic Kolbah, T. and Plavsic, F. (1990) Journal of Pharmaceutical and Biomedical Analysis, 8, 915-918.
[21] López-Alarcón, C., Squella, J.A., Nú?ez-Vergara, L.J., Baez, H. and Camargo, C. (2002) Rapid Communicatios in Mass Spectrometry, 16, 2229-2238.
[22] Martinez, V., Lopez, J.A., Alonso, R.M. and Jimenez, R.M. (1999) Micellar electrokinetic chromatography as a fast screening method for the determination of 1,4-dihy dropyridine calcium antagonists. Journal of Chromatography A, 836, 189-199. doi:10.1016/S0021-9673(98)01029-2
[23] Bretnall, A.E. and Clarke, G.S. (1995) Investigation and optimisation of the use of micellar electrokinetic chro matography for the analysis of six cardiovascular drugs. Journal of Chromatography A, 700, 173-178. doi:10.1016/0021-9673(95)00112-Z
[24] Wang, J., Chen, H. and Zhao L. (1999) Chinese Journal of Analytical Chemistry, 1.
[25] Nishi, H., Fukuyama, T., Matsuo, M. and Terabe S. (1990) Chiral separation of diltiazem, trimetoquinol and related compounds by micellar electrokinetic chromatography with bile salts. Chromatographia, 515, 233-243.
[26] Mohamed Sikkander, A., Vedhi, C. and Manisankar, P. (2011) Electrochemical stripping studies of amlodipine using Mwcnt modified glassy carbon electrode. Chemis try and Materials Research, 1, 1-7.
[27] Stoiljkovi?, Z.?., Avramov Ivi?, M.L., Petrovi?, S.D., Mijin, D.?., Stevanovi?, S.I., La?njevac, U.?. and Marinko vi?, A.D. (2012) Voltammetric and square-wave anodic stripping determination of amlodipine besylate on gold electrode. International Journal of Electrochemical Science, 7, 2288-2303.
[28] Felipe, S.S., Eder, T.G.C. and Christopher, M.A.B. (2009) Electrochemical behavior of verapamil at graphite-poly urethane composite electrodes: Determination of release profiles in pharmaceutical samples. Analytical Letters, 42, 1119-1135. doi:10.1080/00032710902890470
[29] Altiokka, G. and Altiokka, M. (2002) Flow injection analysis of amlodipine using UV-detection. Pharmazie, 57, 500.
[30] El-Didamony, A.M. and Erfan, E.A.H. (2011) Cerimetric determination of four antihypertensive drugs in pharmaceutical preparations. Journal of the Chilean Chemical Society, 56, 875-880. doi:10.4067/S0717-97072011000400011
[31] Vichare, V., Tambe, V., Kashikar, V. and Dhole, S.N. (2011) Simultaneous determination of amlodipinebesy late and hydrochlorothiazide in combined tablet dosage form by simultaneous equation, absorption ratio and first order derivative spectroscopy methods. International Journal of Chemistry Research, 1, 7-10.
[32] Gadallah Mohamed, N. (2011) Simultaneous determination of amlodipine and valsartan. Analytical chemistery insights. Analytical Chemistery Insights, 6, 53-59.
[33] Sahu, R. and Patel, V.B. (2007) Simultaneous spectrophotometric determination of amlodipine besylate and atorvastatin calcium in binary mixture Indian Journal of Phar maceutical Sciences, 69, 110-111. doi:10.4103/0250-474X.32119
[34] Rahman, N. and Azmi, S.N.H. (2000) Spectrophotometric determination of diltiazem hydrochloride with sodium me tavanadate. Microchemical Journal, 65, 39-43. doi:10.1016/S0026-265X(00)00025-4
[35] Sawyer, D.T., Heinman, W.R. and Beebe, J.M. (1984) Chemistry experiments for instrumental methods. John Wiley and Sons Inc., New York.
[36] Inczedy, J. (1976) Analytical application of complex equilibria. 2nd Edition, John Wiley & Sons Inc., Buda pest.
[37] American Pharmaceutical Association (2007) The United States pharmacopeia 29 and NF 25. Washington DC.
[38] David, J.M., Cynthia, L.O. and Shuster, I. (1977) Ana lytical profiles of drug substances and excipients. Academic Press, Inc., Waltham.
[39] Smith, G.P. and Zui, L.C. (1977) Analytical profiles of drug substances and excipients. Academic Press, Inc., Waltham.
[40] Basavaiah, K., Chandrashekar, U. and Prameela, H.C. (2003) Sensitive spectrophotometric determination of am lodipine and felodipine using iron(III) and ferricyanide. II Farmaco, 58, 141-148. doi:10.1016/S0014-827X(02)00018-6
[41] Vogel, E. (1989) Vogel’s text book of quantitative chemical analysis, 5th Edition. In: Jefferry, G.H., Bassett, J., Mendham, J. and Denney, R.C., Eds, Bath Press, Bath.
[42] Topic Q2A (1994) Text on validation of analytical procedures. International Conference on Harmonisation.
[43] Topic Q2B (1996) Validation of analytical procedures: Methodology. International Conference on Harmonisa tion.

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.