The antithrombotic activity of mini-type tomatoes is dependent on the particular variety and the stage of harvest. Lycopene content does not contribute to antithrombotic activity

Junichiro Yamamoto, Hiroyo Ohno, Kanae Hyodo, Masahiko Onishi, John C. Giddings

Cal-Farm, Kobe, Japan.
Cardiff University, Cardiff, UK.
Laboratory of Physiology, Faculty of Nutrition, Kobe Gakuin University, Kobe, Japan.

DOI: 10.4236/health.2013.54090                

Abstract

The prevention of arterial thrombotic disease has a high priority in developed countries. We have focused our studies on the antithrombotic activity of those fruits and vegetables with the potential to prevent the disease, and the present study was undertaken as part of a series of investigations to examine beneficial fruits and vegetables. For this purpose, suitable laboratory tests as well as diets have been devised. In the current investigation, we have classified various tomato varieties with antithrombotic properties, and we now have extended our overall data to include more than ten antithrombotic varieties of fruits and vegetables. A method designed to measure shear-induced platelet activity (the Global Thrombosis Test, GTT) was used to assess haemostasis in vitro and a He-Ne laser-induced thrombosis technique was utilized to examine arterial thrombogenesis in vivo. Concentrations of the antioxidant, lycopene, were also measured. Three mini-type tomato varieties, coded “Cin”, “Pik” and “Caec”, and one mediumtype variety, coded “K”, were harvested at different stages of maturity. All mini-type varieties demonstrated antithrombotic activity at an early (green) stage. The antithrombotic activity decreased with the maturation of “Cin” and “Caec” but remained constant at all stages of maturity with “Pik”. The medium variety, “K”, did not possess antithrombotic activity. Lycopene was not detected at any stage in any of the tomato varieties, suggesting that this antioxidant did not contribute to antithrombotic activity. The present results indicated that the antithrombotic activity of tomatoes is dependent on the particular variety and stage of maturity, and that this activity is not due to lycopene.

Keywords

Tomato; Lycopene; Global Thrombosis Test (GTT); Shear-Induced Platelet Aggregation

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Yamamoto, J., Taka, T., Yamada, K., Ijiri, Y., Murakami, M., Hirata, Y., Naemura, A., Hashimoto, M., Yamashita, T., Oiwa, K., Seki, J., Suganuma, H., Inakuma, T. and Yoshida, T. (2003) Tomatoes have natural antithrombotic effects. British Journal of Nutrition, 90, 1031-1038. doi:10.1079/BJN2003994
[2]	Yamada, K., Naemura, A., Sawashita, N., Noguchi, Y. and Yamamoto, J. (2004) An onion variety has natural anti-thrombotic effect as assessed by thrombosis/thrombolysis. Thrombosis Research, 114, 213-220. doi:10.1016/j.thromres.2004.06.007
[3]	Naemura, A., Mitani, T., Ijiri, Y., Tamura, Y., Yamashita, T., Okimura, M. and Yamamoto, J. (2005) Anti-thrombotic effect of strawberries. Blood Coagulation & Fibrinolysis, 16, 501-509. doi:10.1097/01.mbc.0000184737.50594.a8
[4]	Yamamoto, J., Naemura, A., Ura, M., Ijiri, Y., Yamashita, T., Kurioka, A. and Koyama, A. (2006) Testing various fruits for anti-thrombotic effect: I. Mulberries. Platelets, 17, 555-564. doi:10.1080/09537100600759295
[5]	Yamamoto, J., Naemura, A., Ijiri, Y., Ogawa, K., Suzuki, T., Shimada, Y. and Giddings, J.C. (2008) The antithrombotic effects of carrot filtrates in rats and mice. Blood Coagulation & Fibrinolysis, 19, 785-792. doi:10.1097/MBC.0b013e3283177b19
[6]	Ichiura, D., Naemura, A., Ura, M., Mori, M. and Yamamoto, J. (2009) Antithrombotic effect of potato in animal experiments. Food, 3, 8-12.
[7]	Kinugasa, C., Naemura, A., Hyodo, K., Nakai, Y., Katsuta, M. and Yamamoto, J. (2011) Experimental antithrombotic effects of sesame seed whole grains and extracts. Blood Coagulation & Fibrinolysis, 22, 526-531. doi:10.1097/MBC.0b013e328347b085
[8]	Hyodo, K., Horii, I., Nishino, M., Giddings, J.C. and Yamamoto, J. (2011) The antithrombotic effects of onion filtrates in rats and mice. Health, 3, 319-325. doi:10.4236/health.2011.36055
[9]	Ratnatunga, C.P., Edmondson, S.F., Rees, G.M. and Kovacs, I.B. (1982) High-dose aspirin inhibits shear-induced platelet reaction involving thrombin generation. Circulation, 85, 1077-1082. doi:10.1161/01.CIR.85.3.1077
[10]	Yamamoto, J., Yamashita, T., Ikarugi, H., Taka, T., Hashimoto, M., Ishii, H., Watanabe, S. and Kovacs, I.B. (2003) Gorog Thrombosis Test: A global in-vitro test of platelet function and thrombolysis. Blood Coagulation & Fibrinolysis, 14, 31-39. doi:10.1097/00001721-200301000-00007
[11]	Yamamoto, J. and Kovacs I.B. (2003) Shear-induced invitro haemostasis/thrombosis tests: The benefit of using native blood. Blood Coagulation & Fibrinolysis, 14, 697- 702. doi:10.1097/00001721-200312000-00001
[12]	Kovacs, I.B., Gorog, D. and Yamamoto, J. (2006) Stimulated spontaneous thrombolysis: A new therapeutic challenge. Journal of Thrombosis and Thrombolysis, 21, 221- 227. doi:10.1007/s11239-006-6579-0
[13]	Kovacs, I.B. and Yamamoto, J. (2006) Review: Spontaneous thrombolysis. A forgotten determinant of life or death. Clinical and Applied Thrombosis and Hemostasis, 12, 358-363. doi:10.1177/1076029606291410
[14]	Ijiri, Y., Miura, M., Hashimoto, M., Fukunaga, C., Watanabe, S., Kubota, A., Oiwa, K., Okuda, T. and Yamamoto, J. (2002) A new model to evaluate the diet-induced prothrombotic status, using He-Ne laser-induced thrombogenesis in the carotid artery of apolipoprotein E-deficient and low-density lipoprotein receptor-deficient mice. Blood Coagulation & Fibrinolysis, 13, 497-504. doi:10.1097/00001721-200209000-00004
[15]	Rao, A.V. and Agarwal, S. (2000) Role of antioxidant lycopene in cancer and heart disease. Journal of American College of Nutrition, 19, 563-569.
[16]	Saraf, S., Christopoulos, C., Salha, I.B., Stott, D.J. and Gorog, D.A. (2010) Impaired endogenous thrombolysis in acute coronary syndrome patients predicts cardiovascular death and nonfatal myocardial infarction. Journal of American College of Cardiology, 55, 2107-2115. doi:10.1016/j.jacc.2010.01.033
[17]	Gorog, D.A., Yamamoto, J., Saraf, S., Ishii, H., Ijiri, Y., Ikarugi, H., Wellsted, D.M., Mori, M. and Yamori, Y. (2011) First direct comparison of platelet reactivity and thrombolytic status between Japanese and Western volunteers: Possible relationship to the “Japanese paradox”. International Journal of Cardiology, 152, 43-48. doi:10.1016/j.ijcard.2010.07.002