Preparation and Properties of Probiotic Chocolates Using Yoghurt Powder

Abstract

Milk chocolates were prepared by replacing skim milk powder in the formulation with yoghurt powder at 50% and 100% levels. The effect of incorporating yoghurt powder on quality of chocolates was studied. No significant changes were observed in fatty acid profile and hardness of the chocolates. Sour taste of chocolate with yoghurt powder due to its acidity was neutralized by adding calculated amount of sodium bicarbonate. Sensory analysis showed that probiotic chocolates were highly acceptable and similar to control chocolate. Microbiological studies of chocolates showed the presence of Lactobacillus species to the extent of 3.37 log·cfu/g, which were not present in the control sample prepared only with skim milk powder. Rheological studies showed that milk chocolate prepared using yoghurt powder at 50% showed no significant changes in yield value compared to that of control, but at 100% addition a considerable decrease in yield value was observed. Microstructural properties of chocolate with 50% addition of yoghurt powder showed smaller particles adhering to the cocoa and sugar crystals but at 100% addition of yoghurt powder, the cocoa particles were completely covered by smaller yoghurt powder matrix.

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R. Chetana, S. Reddy and P. Negi, "Preparation and Properties of Probiotic Chocolates Using Yoghurt Powder," Food and Nutrition Sciences, Vol. 4 No. 3, 2013, pp. 276-281. doi: 10.4236/fns.2013.43037.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] S. T. Beckett, “Conching,” In: S. T. Beckett, Eds., Industrial Chocolate Manufacture and Use, 3rd Edition, Blackwell Science, Oxford, 1999, pp. 1-81.
[2] C. L. Hii, C. L. Law, S. Suzannah, S. Miswani and M. Cloke, “Polyphenols in Cocoa (Theobroma cacao L.),” Asian Journal of Food and Agro Industries, Vol. 2, No 4, 2009, pp. 702-722.
[3] S. J. Haylock and T. M. Dodds, “Ingredients from Milk,” In: S. T. Beckett, Eds., Industrial Chocolate Manufacture and Use, 3rd Edition, Blackwell Science, Oxford, 1999, pp. 1-77.
[4] B. German, E. J. Schiffrin, R. Reniero, B. Mollet, A. Pfeifer and J. R. Neeser. “The Development of Functional Foods: Lessons from the Gut,” Trends in Biotechnology, Vol. 17, No. 12, 1999, pp. 492-499.
[5] M. A. F. Belem, “Application of Biotechnology in the Product Development of Nutraceuticals in Canada,” Trends Food Science and Technology, Vol. 10, No. 3, 1999, pp. 101-106. doi:10.1016/S0924-2244(99)00029-1
[6] N. M. Childs, “Nutraceutical Industry Trends,” Journal of Nutraceutical, Functional and Medicinal Foods, Vol. 2, No. 1, 1999, pp. 73-85. doi:10.1300/J133v02n01_07
[7] C. J. Dillard and J. B. German, “Phytochemicals: Nutraceuticals and Human Health,” Journal of Food Agricultural Sciences, Vol. 80, No. 12, 2000, pp. 1744-1756. doi:10.1002/1097-0010(20000915)80:12<1744::AID-JSFA725>3.0.CO;2-W
[8] C. N. Heenan, M. C. Adams, R. W. Hosken and G. H. Fleet, “Survival and Sensory Acceptability of Probiotic Microorganisms in a Non-Fermented Frozen Vegetarian Dessert,” LWT—Food Science & Technology, Vol. 37 No. 4, 2004, pp. 461-466. doi:10.1016/j.lwt.2003.11.001
[9] T. D. Boylston, C. G. Vinderola, H. B. Ghoddusi and J. A. Reinheimer, “Incorporation of Bifidobacteria into Cheeses: Challenges and Rewards,” International Dairy Journal, Vol. 14, No. 5, 2004, pp. 375-387. doi:10.1016/j.idairyj.2003.08.008
[10] V. Xanthopoulos, D. Petridis and N. Tzanetakis, “Characterization and Classification of Streptococcus thermophilus and Lactobaccilus delbrueckii Sub Species Bulgaricus Strains Isolated from Traditional Greek Yoghurt,” Journal of Food Science, Vol. 66, No. 5, 2001, pp. 747-752. doi:10.1111/j.1365-2621.2001.tb04632.x
[11] R. P. Aneja, B. N. Mathur, R. C. Chandan and A. K. Banerjee, “Technology of Indian Milk Products,” Dairy India Year Book Publications, New Delhi, 2002.
[12] M. Kamruzzaman, M. N. Islam and M. M. Rahman, “Shelf life of Different Types of Dahi at Room and Refrigeration Temperature,” Pakistan Journal of Nutrition, Vol. 1, No. 6, 2002, pp. 263-266.
[13] E. Nebesny, D. ?y?elewicz, I. Motyl and Z. Libudzisz, “Properties of Sucrose-Free Chocolates Enriched with Viable Lactic Acid Bacteria,” European Food Research and Technology, Vol. 220, No. 3-4, 2004, pp. 358-362. doi:10.1007/s00217-004-1069-0
[14] S. Possemiers, M. Marzorati, W. Verstraete and T. Van de Wiele, “Bacteria and Chocolate: A Successful Combination for Probiotic Delivery,” International Journal of Food Microbiology, Vol. 141, No. 1-2, 2010, pp. 97-103. doi:10.1016/j.ijfoodmicro.2010.03.008
[15] H. Stone and J. L. Sidel, “Sensory Evaluation Practices,” 3rd Edition, Academic Press/Elsevier, London/New York, 2004.
[16] B. D. Duncan, “Multiple Range and Multiple F-Test,” Biometrics, Vol. 11, No. 1, 1955, pp. 1-42. doi:10.2307/3001478
[17] IOCCC (International Office of Cocoa, Chocolate, and Sugar Confectionery), “Viscosity of Cocoa and Chocolate Products (Analytical Method: 46),” CABISCO, Brussels, 2000.
[18] S. Abbasi, “Food Texture and Viscosity: Concept and Measurement,” Marzedanesh Publishers, Tehran, 2007. (in Farsi)
[19] J. Chevalley, “Chocolate Flow Properties,” In: S. T. Beckett, Ed., “Industrial Chocolate Manufacture and Use,” 3rd Edition, Blackwell Science, Oxford, 1999, pp. 182-200.
[20] W. E. Connor, “Harbingers of Coronary Heart Disease: Dietary Saturated Fatty Acids and Cholesterol. Is Chocolate Benign Because of Its Stearic Acid Content?” American Journal of Clinical Nutrition, Vol. 70, No. 6, 1999, pp. 951-952.
[21] E. Bylaite, P. R. Venskutonis and R. Mapdbieriene, “Properties of Caraway (Carum carvi L.) Essential Oil Encapsulated into Milk Protein-Based Matrices,” European Food Research Technology, 2001, Vol. 212, No. 6, pp. 661-670. doi:10.1007/s002170100297

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