Age changes of antyoxidant system and free-radical processes in rats at formation of postinfarction cardiosclerosis

Tatiana Rebrova, Sergey Afanasiev, Olesia Putrova, Roman Batalov, Sergey Popov

Federal State Budgetary Institution, “Research Institute for Сardiology” of Siberian Branch, The Russian Academy of Medical Sciences, Tomsk, Russia.

DOI: 10.4236/aar.2013.21007                

Abstract

Estimation of age dependent changes of the lipid peroxidation (LPO) intensity, content of stable metabolites of nitrogen oxides (NO) and antioxidant enzyme activities in rat blood serum in conditions of experimental postinfarction cardiosclerosis (PICS) is carried out. Initiation of the postinfarction remodeling of animals has been carried out with coronary occlusion, definition of LPO and NO metabolites indices has been performed after 45 days. Investigations have been carried out on 40 four and twelve-month-old male Wistar rats with mass 200-250 gand 400 -450 g, accordingly. Statistical analysis of the results was performed using the Mann-Whitney-Wilcoxone criterion. It has been found that already intact animals have age specificity of indices under consideration. The expressed activity of LPO processes on the background of reduction of endogenous fermentative antioxidant (SOD and catalase) activity as well as nitrite concentration in blood serum is characteristic for PICS of 4-month-old animals. PICS of 12 month-old rats is accompanied with suppression of the LPO processes on the back- ground of reduction of the antioxidant enzyme intensity and increase of NO metabolites production. The following conclusions have been drawn. Process of ontogenesis is characterized by imbalance between pro-and antioxidant processes in rat blood. Increase in catalase activity and concentration of the TBC-active products at simultaneous decrease of SOD activity and content of diene conjugates has been noted. The organism of young animals responds with persistent increase of LPO processes and decrease of SOD and catalase activity on formation of postinfartion cardioslerosis. The less expressed increase of lipid peroxidation activation and decrease of catalase activity has been noted in the organism of old animals within 45 day after PICS formation.

Keywords

Lipid Peroxidation; Nitrogen Oxide; Postinfarction Cardiosclerosis; Age-Related Aspect

Conflicts of Interest

The authors declare no conflicts of interest.

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