Rapid In-Vitro and In-Vitro Detection of Chalara fraxinea by Means of Mass Spectrometric Techniques

Thi Lam Huong Pham, Irmtraut Zaspel, Michael Schuemann, Heike Stephanowitz, Eberhard Krause

Institute for Forest Genetics (TI), Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.
Institute of Ecology, Berlin University of Technology, Berlin, Germany.
Leibniz Institute for Molecular Pharmacology, Berlin, Germany.
Leibniz Institute for Molecular Pharmacology, Berlin, Germany..

DOI: 10.4236/ajps.2013.42A057                

Abstract

For the first time, mass spectrometric (MS) techniques were employed to rapidly detect the pathogen Chalara fraxinea in-vitro and directly in-vivo in tissues of diseased ash trees caused by C. fraxinea, using a range of characteristic novel secondary metabolites of C. fraxinea as chemical markers for the presence of the pathogen. We have found an evident correlation between the presence and amount of these-only for C. fraxinea characteristic and novel-secondary metabolites (named chalarafraxinines) and the degree of disease of respective infected ash seedlings. As demonstrated in this work, the MS based high-throughput-screening approach constitute an alternative to the time consuming and expensive micro biological isolation procedures for detection of the pathogen C. fraxinea and furthermore, can be used to rapidly test ash genotypes for resistance / susceptibility to C. fraxinea infection.

Keywords

Chalara fraxinea; Rapid In-Vitro; In-Vitro Detection; MS Techniques; MALDI; LC-MS

Conflicts of Interest

The authors declare no conflicts of interest.

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