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Authentication and Genetic Origin of Medicinal Angelica acutiloba Using Random Amplified Polymorphic DNA Analysis

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DOI: 10.4236/ajps.2013.42035    3,919 Downloads   5,166 Views   Citations

ABSTRACT

Some Angelica species are used for medicinal purposes. In particular, the roots of Angelica acutiloba var. acutiloba and A. acutiloba var. sugiyamae, known as Toki and Hokkai Toki, respectively, are used as important medicinal materials in traditional Japanese medicine. However, since these varieties have recently outcrossed with each other, it is difficult to determine whether the Japanese Angelica Root material used as a crude drug is the pure variety. In this study, we developed an efficient method to authenticate A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae from each other and from other Angelica species/varieties. The random amplified polymorphic DNA (RAPD) method efficiently discriminated each Angelica variety. A. acutiloba var. sugiyamae was identified via a characteristic fragment amplified by the decamer primer OPD-15. This fragment showed polymorphisms among Angelica species/varieties. The unique fragment derived from A. acutiloba var. sugiyamae was also found in one strain of A. acutiloba var. acutiloba, implying that this strain arose from outcrossing between A. acutiloba var. acutiloba and A. acutiloba var. sugiyamae. This RAPD marker technique will be useful for practical and accurate authentication among A. acutiloba var. acutiloba, A. acutiloba var. sugiyamae, and their adulterants.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

K. Matsubara, S. Shindo, H. Watanabe and F. Ikegami, "Authentication and Genetic Origin of Medicinal Angelica acutiloba Using Random Amplified Polymorphic DNA Analysis," American Journal of Plant Sciences, Vol. 4 No. 2, 2013, pp. 269-273. doi: 10.4236/ajps.2013.42035.

References

[1] Hirokawa Publishing Co., “The Japanese Pharmacopoeia,” 16th Edition, Hirokawa Publishing Co., Tokyo, 2011.
[2] E. Kaibara, “Chapter Galenicals Toki,” Yamato Honzo, Vol. 6, 1709.
[3] R. Terashima, “Chapter Herbs Toki,” Wakansansazue, Vol. 93, 1713.
[4] H. Hikino, “Bibliographical Research and History of Productions of Toki or Tang kuei 2,” Yakugaku Kenkyu, Vol. 29, 1957, pp. 1059-1078.
[5] Ministry of Health and Welfare, Pharmaceutical Affairs Bureau, “Yakuyou-Shokubutsu Saibai to Hinshitsu-Hyoka Part 1,” Yakuji Nippo, Tokyo, 1992, pp. 41-50.
[6] T. Gotoh, T. Tanaka, T. Kawamura and Y. Noro, “Phytosociological Studies of the Communities on Angelica acutiloba and A. acutiloba var. iwatensis (1) Gifu Prefecture and Its Surroundings,” Natural Medicines, Vol. 49, No. 3, 1995, pp. 255-260.
[7] K. Hatano, I. Nishioka and S. Iwasa, “Cytogenetical Studies of Umbelliferous Plants. (1) The Karyotype and Cross-Compatibility on the Original Plants of Japanese Toki,” Syoyakugaku Zasshi, Vol. 28, No. 1, 1974, pp. 51-64.
[8] K. Matsubara, S. Shindo, H. Watanabe, F. Ikegami, “Identification of Angelica acutiloba and Related Species by Analysis of Inter- and Intra-Specific Sequence Variations in Chloroplast and Nuclear DNA Sequences,” American Journal of Plant Sciences, Vol. 3, No. 9, 2012, pp. 1260-1265. doi:10.4236/ajps.2012.39152
[9] P. Lashermes, J. Cros, P. Marmey and A. Charrier, “Use of Random Amplified DNA Markers to Analyse Genetic Variability and Relationships of Coffea Species,” Genetic Resources and Crop Evolution, Vol. 40, No. 2, 1993, pp. 91-99. doi:10.1007/BF00052639
[10] P. C. Shaw and P. P. H. But, “Authentication of Panax Species and Their Adulterants by Random-Primed Polymerase Chain Reaction,” Planta Medica, Vol. 61, No. 5, 1995, pp. 91-102. doi:10.1055/s-2006-958138
[11] S. E. Wilkie, P. G. Isaac and R. J. Slater, “Random Amplified Polymorphic DNA (RAPD) Markers for Genetic Analysis in Allium,” Theoretical Applied Genetics, Vol. 86, No. 4, 1993, pp. 497-504. doi:10.1007/BF00838566
[12] S. K. Palai and G. R. Rout, “Identification and Genetic Variation among Eight Varieties of Ginger by Using Random Amplified Polymorphic DNA Mark-ers,” Plant Biotechnology, Vol. 420, 2007, pp. 417-420. doi:10.5511/plantbiotechnology.24.417
[13] J. G. K. Williams, A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey, “DNA Polymorphisms Amplified by Arbitrary Primers Are Useful as Genetic Markers,” Nucleic Acids Research, Vol. 18, No. 4, 1990, pp. 6531-6535. doi:10.1093/nar/18.22.6531
[14] L. Congiu, M. Chicca, R. Cella, R. Rossi and G. Bernacchiaet, “The Use of Random Amplified Polymorphic DNA (RAPD) Markers to Identify Strawberry Varieties: A Forensic Application,” Molecular Ecology, Vol. 9, No. 2, 2000, pp. 229-232. doi:10.1046/j.1365-294x.2000.00811.x
[15] M. Kohjyouma, O. Iida, N. Yoshida, Y. Hatakeyama, M. Satake, S. Sekita and H. Kohda, “Random Amplified Polymorphic DNA Analysis of Angelica acutiloba and Its Varieties,” Natural Medicines, Vol. 52, No. 2, 1998, pp. 130-134.
[16] M. W. Lassner, P. Peterson and J. I. Yoder, “Simultaneous Amplification of Multiple DNA Fragments by Polymerase Chain Reaction in the Analysis of Transgenic Plants and Their Progeny,” Plant Molecular Biology Reporter, Vol. 7, No. 2, 1989, pp. 116-128. doi:10.1007/BF02669627
[17] M. Munthali, B. V. Ford-Lloyd and H. J. Newbury, “The Random Amplification of Polymorphic DNA for Fingerprinting Plants,” PCR Methods and Applications, Vol. 1, 1992, pp. 274-276. doi:10.1101/gr.1.4.274
[18] Z. Y. Yang, Z. Chao, K. K. Huo, H. Xie and Z. P. Tian, “ITS Sequence Analysis Used for Molecular Identification of the Bupleurum Species from Northwestern China,” Phytomedicine, Vol. 14, 2007, pp. 416-423. doi:10.1016/j.phymed.2007.04.009

  
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