Localization and Associative Strength of Acid Polysaccharides in Coccoliths of Pleurochrysis haptonemofera (Haptophyta) Predicted from Their Extractability from Partially Decalcified Coccoliths
Yasutaka Hirokawa, Satoru Matsuzuka, Sho Itayama, Tatsuya Uchida, Shoko Fujiwara, Noriaki Ozaki, Hiromichi Nagasawa, Mikio Tsuzuki
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan; Department of Biotechnology, Akita Prefectural University, Akita, Japan.
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan.
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan; 3Japan Science and Technology Agency, CREST, Tokyo, Japan.
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan; Faculty of Science and Engineering, Waseda University, Tokyo, Japan.
DOI: 10.4236/ojms.2013.31005   PDF    HTML   XML   3,533 Downloads   6,668 Views   Citations

Abstract

The coccolithophorids have calcified scales called coccoliths on their surface that include abundant acid polysaccharides. To determine the localization and associative strength of acid polysaccharides Ph-PS-1, -2, and -3 incoccoliths of Pleurochrysis haptonemofera, we analyzed the acid polysaccharides extracted with urea from partially decalcified coccoliths. On treatment of coccoliths with8.0 Murea at pH8.0 inboiling water, Ph-PS-2 was not extracted, but parts of Ph-PS-1 and -3 were from the surface without the crystal morphology being affected. When coccoliths were partially decalcified at various pHs (pH 8.0 - 5.0), Ph-PS-1, -3, and -2 were extracted with urea in that order as the calcite crystals dissolved. Detection of the acid polysaccharides using fluorescein-isothiocyanate-labeled lectin (ConA) and anti-Ph-PS-2 antibodies demonstrated that Ph-PS-2 exists on the surfaces of both untreated and urea-treated coccoliths. Moreover, NaClO-treatment under moderate conditions extracted only Ph-PS-1, -3, and part of Ph-PS-2 from coccoliths, without separation of the crystal units, suggesting that at least some part of Ph-PS-2 acts as a glue connecting crystal units and/or a crystal unit and a base plate inside coccoliths. These results suggest that Ph-PS-2 exists not only on the surface but also inside of the coccoliths, most of it being strongly bound to coccoliths, while Ph-PS-1 and -3 are weakly associated close to the coccolith surface, in that order from the surface. This strongly supports a scheme in which PS-2 type acid polysaccharides play an important role in the crystal nucleation and PS-3 type functions during crystal growth.

 

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Y. Hirokawa, S. Matsuzuka, S. Itayama, T. Uchida, S. Fujiwara, N. Ozaki, H. Nagasawa and M. Tsuzuki, "Localization and Associative Strength of Acid Polysaccharides in Coccoliths of Pleurochrysis haptonemofera (Haptophyta) Predicted from Their Extractability from Partially Decalcified Coccoliths," Open Journal of Marine Science, Vol. 3 No. 1, 2013, pp. 48-54. doi: 10.4236/ojms.2013.31005.

Conflicts of Interest

The authors declare no conflicts of interest.

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