Quality of Canola Oil Obtained by Conventional and Supercritical Fluid Extraction

Abstract

Quality of canola oil obtained by the supercritical fluid extraction (SFE), using CO2 with ethanol as a co-solvent, was evaluated and compared to that of the conventionally-obtained oils using either n-hexane or chloroform methanol mixture. Physical characteristics, chemical properties, fatty acid composition and phenolic profile of oils were investigated. The SFE oil showed significantly lower melting point, peroxide value (PV) and higher free fatty acids (FFAs) and iodine value (IV) than the n-hexane-extracted one. There were no significant differences in the fatty acid composition of different oils. The SFE oil showed significantly higher phenolic content (35.91, 10.15, 3.16, 0.32 and47.48mg/g of sinapic acid, sinapine, sinapoyl glucose, canolol and total phenolics) as compared to 0.08, 0.70, 0.88, 0.45 and0.71mg/g, respectively in the n-hexane-extracted oil. These results indicate the superiority of SFE and advocate its use for the extraction of highly stable and functional canola oil for further health and nutraceutical uses. The present results have an industrial and technological relevance as SFE could be competitive with the traditional extraction techniques providing an environmental approach and enhancing the obtained oil quality and stability. After recovery of the initial installation costs, SFE could be more economic than conventional extraction. However, further economical studies are needed to validate this last conclusion.

Share and Cite:

R. Khattab, C. Rempel, M. Suh and U. Thiyam, "Quality of Canola Oil Obtained by Conventional and Supercritical Fluid Extraction," American Journal of Analytical Chemistry, Vol. 3 No. 12A, 2012, pp. 966-976. doi: 10.4236/ajac.2012.312A128.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Statistics Canada, “Cereals and Oilseeds Review,” Minister of Industry, Vol. 35, No. 6, 2012, p. 31.
[2] R. Rice, “Mediterranean Diet,” Lancet, Vol. 344, No. 8926, 1994, pp. 893-894. HUdoi:10.1016/S0140-6736(94)92869-XU
[3] World Health Organization, “Life in 21st Century. A Vision for All, World Health Report,” WHO, Geneve, 1994.
[4] R. G. Ackman, “Canola Fatty Acids—An Ideal Mixture for Health, Nutrition and Food Use,” In: F. Shahidi, Ed., Canola and Rapeseed: Production, Chemistry, Nutrition and Processing Technology, Van Nostrand Reinhold, New York, 1994, pp. 81-89.
[5] M. Naczk, R. Amarowicz, A. Sullivan and F. Shahidi, “Current Research Developments on Polyphenolics of Rapeseed/Canola: A Review,” Food Chemistry, Vol. 62, No. 4, 1998, pp. 489-502. HUdoi:10.1016/S0308-8146(97)00198-2U
[6] H. Kozlowska, M. Naczk, F. Shahidi and R. Zadernowski, “Phenolic Acids and Tannin in Rapeseed and Canola,” In: F. Shahidi, Ed., Canola and Rapeseed: Production, Chemistry, Nutrition and Processing Technolog, Van Nostr- and Reinhold, New York, 1994, pp. 193-210.
[7] A. Koski, E. Psomiadou, M. Tsimidou, A. Hopia, P. Kefalas, K. Wahala and M. Heinonen, “Oxidative Stability and Minor Constituents of Virgin Olive Oil and Cold-Pressed Rapeseed Oil,” European Food Research and Technology, Vol. 214, No. 4, 2002, pp. 294-298. HUdoi:10.1007/s00217-001-0479-5U
[8] A. Koski, S. Pekkarinen, A. Hopia, K. Wahala and M. Heinonen, “Processing of Rapeseed Oil: Effects on Sinapic Acid Derivative Content and Oxidative Stability,” European Food Research and Technology, Vol. 217, No. 2, 2003, pp. 110-114. HUdoi:10.1007/s00217-003-0721-4U
[9] H. Kuwahara, A. Kanazawa, D. Wakamatu, S. Morimura, K. Kida, T. Akaike and H. Maeda, “Antioxidant and Antimutagenic Activities of 4-Vinyl-2, 6-Dimethoxyphenol (Canolol) Isolated from Canola Oil,” Journal of Agricultural and Food Chemistry, Vol. 52, No. 14, 2004, pp. 4380-4387. HUdoi:10.1021/jf040045+U
[10] H. Nowak, R. Kujava, R. Zadernowski, B. Roczniak and H. Kozlowska, “Antioxidative and Bactericidal Properties of Phenolic Compounds in Rapeseeds,” Fat Science and Technology, Vol. 94, No. 4, 1992, pp. 149-152.
[11] U. N. Wanasundara and F. Shahidi, “Canola Extract as an Alternative Natural Antioxidant for Canola Oil,” Journal of the American Oil Chemists’ Society, Vol. 71, No. 8, 1994, pp. 817-822.
[12] DGF, “Deutsche Einheitsmethoden zur Untersuchung von Fetten, Fettprodukten, Tensiden und verwandten Stoffen,” Wissenschaftliche Verlagsgesellschaft, Stuttgart, 1998.
[13] AOCS, “Official Methods and Recommended Practices of the AOCS,” 6th Edition, American Oil Chemists’ Society, Champaign, 2004.
[14] International Organization for Standardization, “Oilseeds—Determination of Hexane Extract (or Light Petroleum Extract), Called ‘Oil Content’,” International Organization for Standardization, Genève, 1988.
[15] FOSFA, “Determination of Oil Content in Oilseeds—Solvent Extraction, Reference Method,” FOSFA International Manual, Part 2, Standard Contractual Method 45- 50, 1998, pp. 283-288.
[16] L. A. Johnson and E. W. Lusas, “Comparison of Alternative Solvents for Oils Extraction,” Journal of the American Oil Chemists’ Society, Vol. 60, No. 2, 1983, pp. 181- 193.
[17] F. Temelli and N. T. Dunford, “The Effect of Processing Parameters on Extraction of Canola Oil and Phospholipids Using Supercritical Carbon Dioxide,” In: S. S. Koseoglu, K. C. Rhee and R. F. Wilson, Eds., Advances in Oils and Fats, Antioxidants, and Oilseed By-Product, AOCS Press, Champaign, 2004, pp. 25-31.
[18] D. Anderson, “A Primer on Oils Processing Technology,” In: Y. H. Hui, Ed., Bailey’s Industrial Oil and Fat Products, Wiley-Interscience, New York, 1996, pp. 124-125.
[19] J. P. Friedrich, G. R. List and A. J. Heakin, “Petroleum- Free Extraction of Oil from Soybeans with Supercritical CO2,” Journal of the American Oil Chemists’ Society, Vol. 59, No. 7, 1982, pp. 288-292.
[20] S. L. Taylor, J. W. King and G. R. List, “Determination of Oil Content in Oilseeds by Analytical Supercritical Fluid Extraction,” Journal of the American Oil Chemists’ Society, Vol. 70, No. 4, 1993, pp. 437-439.
[21] L. Bruhl and B. Matthaus, “Extraction of Oilseeds by SFE—A Comparison with Other Methods for Determination of the Oil Content,” Fresenius Journal of Analytical Chemistry, Vol. 364, No. 7, 1999, pp. 631-634. HUdoi:10.1007/s002160051399U
[22] V. J. Barthet and J. K. Daun, “An Evaluation of Supercritical Fluid Extraction as an Analytical Tool to Determine Fat in Canola, Flax, Solin, and Mustard,” Journal of the American Oil Chemists’ Society, Vol. 79, No. 3, 2002, pp. 245-251.
[23] J. P. Friedrich and E. H. Pryde, “Supercritical CO2 Extraction of Lipid-Bearing Materials and Characterization of the Products,” Journal of the American Oil Chemists’ Society, Vol. 61, No. 2, 1984, pp. 223-228.
[24] M. Fattori, N. R. Bulley and A. Meisen, “Carbon Dioxide Extraction of Canola Seed: Oil Solubility and Effect of Seed Treatment,” Journal of the American Oil Chemists’ Society, Vol. 65, No. 6, 1988, pp. 968-974.
[25] M. A. Gomez and M. D. L. Ossa, “Quality of Wheat Germ Oil Extracted by Liquid and Supercritical Carbon Dioxide,” Journal of the American Oil Chemists’ Society, Vol. 77, No. 9, 2000, pp. 969-974.
[26] N. T. Dunford and F. Temelli, “Extraction of Phospholipids from Canola with Supercritical Carbon Dioxide and Ethanol,” Journal of the American Oil Chemists’ Society, Vol. 72, No. 9, 1995, pp. 1009-1015.
[27] R. Przybylski, Y. C. Lee and I. H. Kim, “Oxidative Stability of Canola Oils Extracted with Supercritical Carbon Dioxide,” LWT—Food Science and Technology, Vol. 31, No. 7-8, 1998, pp. 687-693.
[28] Z. Quancheng, S. Guihua, J. Hong and W. Moucheng, “Concentration of Tocopherols by Supercritical Carbon Dioxide with Co-Solvent,” European Food Research and Technology, Vol. 219, No. 4, 2004, pp. 398-402. HUdoi:10.1007/s00217-004-0968-4U
[29] E. Jenab, K. Rezaei and Z. Emam-Djomeh, “Canola Oil Extracted by Supercritical Carbon Dioxide and a Commercial Organic Solvent,” European Journal of Lipid Science and Technology, Vol. 108, No. 6, 2006, pp. 488-492. HUdoi:10.1002/ejlt.200600026U
[30] U. N. Wanasundara, R. Amarowicz and F. Shahidi, “Partial Characterization of Natural Antioxidants in Canola Meal,” Food Research International, Vol. 28, No. 6, 1995, pp. 525-530. HUdoi:10.1016/0963-9969(96)87362-5U
[31] U. Thiyam, H. Stockmann and K. Schwarz, “Antioxidant Activity of Rapeseed Phenolics and Their Interactions with Tocopherols during Lipid Oxidation,” Journal of the American Oil Chemists’ Society, Vol. 83, No. 6, 2006, pp. 523-528.
[32] J. Folch, M. Lees and G. H. Stanley, “A Simple Method for Isolation and Purification of Total Lipides from Animal Tissues,” Journal of Biological Chemistry, Vol. 226, No. 1, 1957, pp. 497-509.
[33] H. Li, J. Wu, C. Rembel and U. Thiyam, “Effect of Operating Parameters on Oil and Phenolic Extraction Using Supercritical CO2,” Journal of the American Oil Chemists’ Society, Vol. 87, No. 9, 2010, pp. 1081-1089.
[34] I. S. Saguy, A. Shani, P. Weinberg and N. Garti, “Utilization of Jojoba Oil for Deep-Fat Frying of Foods,” LWT—Food Science and Technology, Vol. 29, No. 5-6, 1996, pp. 573-577.
[35] W. R. Morrison and L. M. Smith, “Preparation of Fatty Acid Methyl Esters and Dimethylacetals from Lipids with Boron Fluoride-Methanol,” Journal of Lipid Research, Vol. 5, No. 4, 1964, pp. 600-608.
[36] R. Khattab, M. Eskin, M. Aliani and U. Thiyam, “Determination of Sinapic Acid Derivatives in Canola Extracts Using High Performance Liquid Chromatography,” Journal of the American Oil Chemists’ Society, Vol. 87, No. 2, 2010, pp. 147-155.
[37] J. Shi, S. Kassama and Y. Kaduda, “Supercritical Fluid Extraction Technology,” In: J. Shi, Ed., Functional Food Ingredients and Nutraceuticals: Processing Technologies, CRC Press Taylor, Boca Raton, 2007, pp. 3-43.
[38] R. E. Timms, “Physical Properties of Oils and Mixtures of Oils,” Journal of the American Oil Chemists’ Society, Vol. 62, No. 2, 1985, pp. 241-248.
[39] R. G. Ackman and C. A. Eaton, “Specific Gravities of Rapeseed and Canbra Oils,” Journal of the American Oil Chemists’ Society, Vol. 54, No. 10, 1977, pp. 435-439.
[40] Canola Council of Canada, “Canola Oil: Physical and Chemical Properties,” 2009. https://canolaouncil.merchantsecure.com/uploads/Chemical1-6.pdf
[41] G. Knothe and R. O. Dunn, “A Comprehensive Evaluation of the Melting Points of Fatty Acids and Esters Determined by Differential Scanning Calorimetry,” Journal of the American Oil Chemists’ Society, Vol. 86, No. 9, 2009, pp. 843-856.
[42] O. O. Fasina, M. Craig-Schmidt, Z. Colley and H. Hallman, “Predicting Melting Characteristics of Vegetable Oils from Fatty Acid Composition,” LWT—Food Science and Technology, Vol. 41, No. 8, 2008, pp. 1501-1505. HUdoi:10.1016/j.lwt.2007.09.012U
[43] U. Riiner, “Investigation on the Polymorphism of Fats and Oil by Temperature Programmed X-Ray Diffraction,” LWT—Food Science and Technology, Vol. 3, 1970, pp. 101-106.
[44] R. Farhoosh, R. E. Kenari and H. Poorazrang, “Frying Stability of Canola Oil Blended with Palm Olein, Olive, and Corn Oils,” Journal of the American Oil Chemists’ Society, Vol. 86, No. 1, 2009, pp. 71-76.
[45] Canola Council of Canada, “Standards and Regulations,” 2009. http://www.canolacouncil.org/uploads/Standards1-2.pdf
[46] H. Kim and S. H. Yoon, “Effect of Extraction Solvents on Oxidative Stability of Crude Soybean Oil,” Journal of the American Oil Chemists’ Society, Vol. 67, No. 3, 1990, pp. 165-167.
[47] L. Brunetti, A. Daghetta, E. Fedeli, I. Kikic and L. Zanderighi, “Deacidification of Olive Oils by Supercritical Carbondioxide,” Journal of the American Oil Chemists’ Society, Vol. 66, No. 2, 1989, pp. 209-217.
[48] H. Rui, L. Zhang, Z. Li and Y. Pan, “Extraction and Characteristics of Seed Kernel Oil from White Pitaya,” Journal of Food Engineering, Vol. 93, No. 4, 2009, pp. 482- 486. HUdoi:10.1016/j.jfoodeng.2009.02.016U
[49] J. E. Kinsella, J. L. Shimp and J. Mai, “The Proximate and Lipid Composition of Several Species of Freshwater Fishes,” New York Food and Life Sciences Bulletin, No. 69, 1978, pp. 1-20.
[50] E. N. Frankel, “Chemistry of Autoxidation: Mechanism, Products and Flavor Significance,” In: D. B. Min and T. H. Smouse, Eds., Flavor Chemistry of Fats and Oils, AOCS, Champaign, 1985, pp. 1-37.
[51] R. Eggers and U. Sievers, “Processing Oilseed with Supercritical Carbon Dioxide,” Journal of Chemical Engineering of Japan, Vol. 22, No. 6, 1989, pp. 641-649. HUdoi:10.1252/jcej.22.641U
[52] B. Bozan and F. Temelli, “Supercritical CO2 Extraction of Flaxseed,” Journal of the American Oil Chemists’ Society, Vol. 79, No. 3, 2002, pp. 231-235.
[53] C. Wijesundera, C. Ceccato, P. Fagan and Z. Shen, “Seed Roasting Improves the Oxidative Stability of Canola (B. napus) and Mustard (B. juncea) Seed Oils,” European Journal of Lipid Science and Technology, Vol. 110, No. 4, 2008, pp. 360-367. HUdoi:10.1002/ejlt.200700214U

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.