The attachment of Staphylococcus epidermidis on the surface of a carbon paste electrode at various positive potentials: The effect of pH, incubation time, and solid-medium type

Dai Long Vu, Libor Červenka, Jana Vavřičková

Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic.

DOI: 10.4236/jbise.2012.512087                

Abstract

The attachment of Staphylococcus epidermidis to the surface of carbon paste electrode (CPE) by applying positive potentials (50 - 600 mV) with regard of various buffer pH, cultivation time and solid-medium type was studied. The attachment process was analyzed by measuring the electric current derived from the dye (amido black) adsorbed on the vacant areas of CPE after attachment of microbial cells. The pH was not identified as a single main factor affecting the attachment (p > 0.05), however further insight revealed that the potentials applied had different effects on the microbial cells attachment. Both cultivation time and solid-medium type significantly affected the microbial attachment. Generally, increase of cultivation time up to 168 h resulted in increase of adhesion. Applying potentials 300, 400 and 200 mV resulted in the highest attachment process for bacteria cultivated for 24, 48 and 168 h, respectively. S. epidermidis cultivated on the blood agar and Baird-Parker agar plates showed the higher extent of attachment.

Keywords

Carbon Paste Electrode; Attachment; Staphylococcus epidermidis

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Araújo, E.A., de Andrade, N.J., da Silva, L.H.M., et al. (2010) Control of microbial adhesion as a strategy for food and bioprocess technology. Food and Bioprocess Technology, 3, 321-332. doi:10.1007/s11947-009-0290-z
[2]	Kordialik-Bogacka, E. (2011) Surface properties of yeast cells during heavy metal biosorption. Central European Journal of Chemistry, 9, 348-351. doi:10.2478/s11532-011-0008-8
[3]	Shi, X. and Zhu, X. (2009) Biofilm formation and food safety in food industries. Trends in Food Science and Technology, 20, 407-413. doi:10.1016/j.tifs.2009.01.054
[4]	Busscher, H.J. and Weerkamp, A.H. (2009) Specific and non-specific interactions in bacterial adhesion to solid substrata. FEMS Microbiology, 46, 165-173. doi:10.1111/j.1574-6968.1987.tb02457.x
[5]	Nostro, A., Cellini, L., di Giulio, M., et al. (2012) Effect of alcaline pH on staphylococcal biofilm formation. Acta Pathologica, Microbiologica, et Immunologica Scandi- navica, 120, 733-742.
[6]	Oh, Y.J., Jo, W., Yang, Y., et al. (2007) Influence of culture conditions on Escherichia coli O157:H7 biofilm formation by atomic force microscopy. Ultramicroscopy, 107, 869-874. doi:10.1016/j.ultramic.2007.01.021
[7]	Sheng, X., Ting, Y.P. and Pehkonen, S.O. (2008) The influence of ionic strenght, nutrients and pH on bacterial adhesion to metals. Journal of Colloid and Interface Science, 321, 256-264. doi:10.1016/j.jcis.2008.02.038
[8]	Speranza, B., Corbo, M.R. and Sinigaglia, M. (2011) Effects of nutritional and environmental conditions on Salmonella spp. biofilm formation. Journal of Food Science, 76, M12-M16. doi:10.1111/j.1750-3841.2010.01936.x
[9]	Tang, J., Chen, J., Liu, J., et al. (2012) Effects of different cultivation conditions on Staphylococcus aureus biofilm formation and diversity of adhesion genes. Journal of Food Safety, 32, 210-218. doi:10.1111/j.1745-4565.2012.00370.x
[10]	Tresse, O., Lebret, V., Benezech, T., et al. (2006) Comparative evaluation of adhesion, surface properties and surface protein composition of Listeria monocytogenes strains after cultivation at constant pH of 5 and 7. Journal of Applied Microbiology, 101, 53-62. doi:10.1111/j.1365-2672.2006.02968.x
[11]	Jones, D.S., Adair, C.G., Mawhinney, W.M., et al. (1996) Standardization and comparison of methods employed for microbial cell surface hydrophobicity and charge determination. International Journal of Pharmaceutics, 131, 83-89. doi:10.1016/0378-5173(95)04368-3
[12]	Torimura, M., Itoet, S., Kano, K., et al. (1999) Surface characterization and online activity measurements of microorganisms by capillary zone electrophoresis. Journal of Chromatography B, 721, 31-37. doi:10.1016/S0378-4347(98)00490-3
[13]	Morisaki, H., Sugimoto, M. and Shiraishi H. (2000) Attachment of bacterial cells to carbon electrodes. Bioelectro-chemistry, 51, 21-25. doi:10.1016/S0302-4598(99)00082-3
[14]	Vu, D.L., Sys, M. and ?ervenka, L. (2012) The effect of various potentials on the attachment of Saccharomyces cerevisiae and Staphylococcus epidermidis to carbon paste electrodes. International Journal of Electrochemical Science, 6, 5265-5274. http://www.electrochemsci.org/papers/vol6/6115265.pdf
[15]	?vancara, I. and Metelka, R. (2000) Piston-driven carbon paste holders for electrochemical measurements. In: Vy- t?as, K. and Kalcher, K. Eds., Sensing in Electroanalysis, University of Pardubice, Pardubice, 7-18.
[16]	Xie, X.H., Li, E.L. and Tang, Z.K. (2010) EQCM and EIS study of the effect of potential of zero charge on Escherichia coli biofilm development. International Journal of Electrochemical Science, 5, 1018-1025. http://www.electrochemsci.org/papers/vol5/5071018.pdf
[17]	K?odzińska, E., Szumski, M., Dziubakiewicz, E., et al. (2010) Effect of zeta potential value on bacterial behavior during electrophoretic separation. Electrophoresis, 31, 1590-1596. doi:10.1002/elps.200900559
[18]	Matsuo, M., Oogai, Y., Kato, F., et al. (2011) Growth- phase dependence of susceptibility to antimicrobial peptides in Staphylococcus aureus. Microbiology, 157, 1786- 1797. doi:10.1099/mic.0.044727-0
[19]	Vanderijn, I. (1986) Quantitative analysis of cell walls of nutritionally variant staphylococci grown under various growth conditions. Infection and Immunity, 49, 518-522.
[20]	Bakholdina, S.I., Krasikova, I.N. and Soloveva, T.F. (2001) The effect of the cultivation method and the growth phase on the lipopolysachcaride composition of Yersinia pseudotuberculosis. Russian Journal of Bioorganic Chemistry, 27, 130-134. doi:10.1023/A:1011341405310
[21]	Kotyńska, J., Petelska, A.D., Szeremeta, M., et al. (2012) Changes in surface-charged density of blood cells after sudden unexpected death. Journal of Membrane Biology, 245, 185-190. doi:10.1007/s00232-012-9428-4
[22]	Anderson, N.W., Buchan, B.W., Riebe, K.M., et al. (2012) Effects of solid-medium type on routine identification of bacterial isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Clinical Microbiology, 50, 1008-1013. doi:10.1128/JCM.05209-11