Schiff base SH11 with tuberculostatic and radical scavenging activities against INH-induced oxidative hepatic damage

Nedyalka Georgieva, Zvezdelina Yaneva, Galina Nikolova, Svetlana Simova

Department of Medical Chemistry and Biochemistry, Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria.
Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, Students Campus, Stara Zagora, Bulgaria.
Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria.

DOI: 10.4236/abb.2012.327130                

Abstract

By in vitro visible electron paramagnetic resonance (EPR) spectrophotometry method we demonstrated that N-isonicotinoyl-N’-(3-etoxy-2-hydroxybenzaldehyd) hydrazone (SH11) exhibits radical scavenging activity (SSA). Malondialdehyde (MDA) in mice treated with INH was increased (2.578 ± 0.349 mM vs. 2.024 ± 0.164 μM, p < 0.001), while both superoxide dismutase (SOD) (1.583 ± 0.562 U_SOD/mg Pr vs. 2.273 ± 0.317 U_SOD/mg Pr, p < 0.05) and catalase (CAT) (30.176 ± 7.300 U_CAT/mg Pr, vs. 47.070 ± 16.490 U_CAT/mg Pr, p < 0.05) were decreased, compared to the untreated controls. The combination INH + SH11 (30 mg/kg p.o.) showed reduced levels of MDA, compared to the INH-treated (mean 2.291 ± 0.025 μM than 2.578 ± 0.349, p < 0.05). The combination with the lowest reduction of SOD compared to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11, but there was a significant difference in SOD activities between the group treated with this combination and the untreated controls (p < 0.05). The most effective combination, with CAT levels, close to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11.

Keywords

INH; Schiff Base; Tuberculosis; EPR; Lipid Peroxidation; Antioxidant Enzymes

Conflicts of Interest

The authors declare no conflicts of interest.

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