Oxidative stress in brickmakers of Juárez City, Chihuahua, México: Case-control study
Leobardo Manuel Gómez-Oliván, Alba Yadira Corral-Avitia, Katya Aimeé Carrasco-Urrutia, Navor Armando González-Granados, Marcela Galar-Martínez, Nadia Neri-Cruz, Hariz Islas-Flores, Octavio Dublan-García
Facultad de Enfermería y Nutriología, Universidad Autónoma de Chihuahua, Chihuahua, México.
Laboratorio de Ciencias Ambientales, Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Juárez, México.
Laboratorio de Toxicología Acuática, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México City, México.
Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Toluca, México.
DOI: 10.4236/abb.2012.327128   PDF    HTML     4,227 Downloads   7,269 Views   Citations

Abstract

A case-control study was conducted in a brickmaker’s community in Juarez City, Chihuahua in Mexico. This population has been chronically exposed to a wide spectrum of potentially health-damaging pollutants that include coarse, fine and ultrafine particles, carbon monoxide (CO), oxides of nitrogen and sulphur, transitional metals, polycyclic aromatic hydrocarbons (PAHs), volatile organic compounds and bioaerosols. Lipid peroxidation level (LPX) and protein carbonyl content (PCC) help to evaluate oxidized protein content and activity of the antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were evaluated in blood samples of study participants. The group of occupationally exposed (OE) workers consisted of 30 individuals ranging in age from 25 to 55 years, with a mean of 15 years in a brick production-related job. The control group included 30 volunteers who were neither environmentally nor occupationally exposed to brick production activities and whose sociodemo-graphic characteristics were similar to the OE group. Results indicate that none of workers used any type of protective equipment (respirator or face mask, gloves, coveralls) during brick manufacturing. LPX and PCC significantly increased (p < 0.5) in the OE group compared to the control group. SOD, CAT and GPx activity was significantly increased (p < 0.5) in the OE group compared to the control group. Therefore, evaluation of stress oxidative biomarkers is advisable in order to assay chronic exposure to workers in brick manufacturing.

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Gómez-Oliván, L. , Corral-Avitia, A. , Carrasco-Urrutia, K. , González-Granados, N. , Galar-Martínez, M. , Neri-Cruz, N. , Islas-Flores, H. and Dublan-García, O. (2012) Oxidative stress in brickmakers of Juárez City, Chihuahua, México: Case-control study. Advances in Bioscience and Biotechnology, 3, 1051-1059. doi: 10.4236/abb.2012.327128.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Fullerton, D.G., Bruce, N. and Gordon, S.B. (2008) Indoor air pollution from biomass fuel smoke is a major health concern in the developing world. Transactions of the Royal Society of Tropical Medicine and Hygiene, 102, 843-851. doi:10.1016/j.trstmh.2008.05.028
[2] Lin, H.H., Ezzati, M. and Murray, M. (2007) Tobacco smoke, indoor air pollution and tuberculosis: A systematic review and metaanalysis. PLoS Med, 4, e20. doi:10.1371/journal.pmed.0040020
[3] Laden, F., Schwartz, J., Speizer, F.E. and Dockery, D.W. (2006) Reduction in fine particulate air pollution and mortality: Extended follow-up of the Harvard Six Cities study. American Journal of Respiratory and Critical Care Medicine, 173, 667-672. doi:10.1164/rccm.200503-443OC
[4] Pope III, C.A., Burnett, R.T., Thun, M.J., Calle, E.E., Krewski, D., Ito, K. and Thurston, G.D. (2002). Lung cancer, cardiopulmonary mortality, and long-term exposure to fine particulate air pollution. Journal of the American Medical Association, 287, 1132-1141. doi:10.1001/jama.287.9.1132
[5] Corral-Avitia, A.Y. and De la Mora-Covarrubias, A. (2012) Environmental assessment of brick kilns in Chihuahua State, Mexico, using digital cartography. In: Mahamane, A., Ed., The Functioning of Ecosystem, Rijeka, Croatia, 271-282.
[6] Sinha, S.N., Kulkarni, P.K., Shah, S.H., Desai, N.M., Patel, G.M., Mansuri, M.M. and Saiyed, H.N. (2006) Environmental monitoring of benzene and toluene produced in indoor air due to combustion of solid biomass fuels. Science Total Environmental, 357, 280-287. doi:10.1016/j.scitotenv.2005.08.011
[7] Naeher, L.P. Brauer, M., Lipsett, M., Zelikoff, J.T., Simpson, C.D., Koenig, J.Q., and Smith, K.R. (2007) Woodsmoke health effects: A review. Inhalation Toxicology, 19, 67-106. doi:10.1080/08958370600985875
[8] Straif, K., Baan, R., Grosse, Y., Secretan B., Ghissassi, F. and Cogliano,V. (2006) WHO International Agency for Research on Cancer Monograph Working Group. Carcinogenicity of household solid fuel combustion and of high-temperature frying. Lancet Oncology, 7, 977-978. doi:10.1016/S1470-2045(06)70969-X
[9] Danielsen, S., Loft, A., Kocbach, P., Schwarze, E. and Moller, P. (2009) Oxidative damage to DNA and repair induced by Norwegian wood smoke particles in human A549 and THP-1 cell lines. Mutation Research, 674, 116-122. doi:10.1016/j.mrgentox.2008.10.014
[10] Bruce, C.W., Corral, A.Y. & Lara, A.S. (2007) Development of cleaner burning brick kilns in Ciudad Juarez, Chihuahua, México. Journal of the Air & Waste Management Association, 57, 444-456. doi:10.3155/1047-3289.57.4.444
[11] Blackman, A., Newbold, S., Shih, J.-S. Cook, J. (2000) The benefits and costs of informal sector pollution control: Mexican brick kilns. Resources for the Future. Washington DC.
[12] Barata, C., Varo, I., Navarro, J.C., Arun S. and Porte, C. (2005) Antioxidant enzyme activities and lipid peroxidation in the freshwater cladoceran Daphnia magna exposed to redox cycling compounds. Comparative Bio-chemistry and Physiology, 140, 175-186.
[13] Abdollahi, M., Ranjbar, A., Shadnia. S., Nikfar, S. and Rezaie, A. (2004) Pesticides and oxidative stress: A review. Medical Science Monitor, 10, 141-147.
[14] Livingstone, D.R. (2003) Oxidative stress in aquatic organisms in relation to pollution and aquaculture. Revue de Medicine Veterinaire, 154, 427-430.
[15] Halliwell, B. and Gutteridge, J.M.C. (1999) Free Radicals in Biology and Medicine. 3rd Edition, Oxford University Press, Oxford.
[16] Rikans, L.E. and Hornbrook, K.R. (1997) Lipid peroxidetion, antioxidant protection and aging. Biochimical et Biophysical Acta, 1362, 116-127. doi:10.1016/S0925-4439(97)00067-7
[17] Büege, J.A. and Aust, S.D. (1978) Microsomal lipid per-oxidation. Methods in Enzymology, 52, 302-310. doi:10.1016/S0076-6879(78)52032-6
[18] Levine, R.L., Williams, J.A., Stadtman, E.R. and Shacter, E. (1994) Carbonyl assays for determination of oxidatively modified proteins. Methods in Enzymology, 233, 346-357. doi:10.1016/S0076-6879(94)33040-9
[19] Misra, H.P. and Fridovich, I. (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry, 247, 3170-3175.
[20] Radi, R., Turrens, J.F., Chang, L.Y., Bush, K.M., Carpo, J.D. and Freeman, B.A. (1991) Detection of catalase in rat heart mitochondria. Journal of Biological Chemistry, 266, 22028-22034.
[21] Gunzler, W. and Flohe-Clairborne, A. (1985) Glutathione peroxidase. In: Green-Wald, R.A., Ed., Handbook of Methods for Oxygen Radical Research, CRC Press, Boca Ratón, 285-290.
[22] Bradford, M. (1976) A rapid and sensitive method for the quantitation of microorganism quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248-254. doi:10.1016/0003-2697(76)90527-3
[23] Bertini, I. and Cavallaro, G. (2008) Metals in the “omics” world: Copper homeostasis and cytochrome c oxidase assembly in a new light. Journal of Biological Inorganic Chemistry, 13, 3-14. doi:10.1007/s00775-007-0316-9
[24] Cairo, G., Recalcati, S., Pietrangelo, A. and Minotti, G. (2002) The iron regulatory protein: Targets and modulators of free radical reactions and oxidative damage. Free Radical Biology and Medicine, 32, 1237-1243. doi:10.1016/S0891-5849(02)00825-0
[25] Cornejo, P., Tapia, G., Puntarulo, S., Galleano, M., Videla, L.A. and Fernandez, V. (2001). Iron-induced changes in nitric oxide and superoxide radical generation in rat liver after lindane or thyroid hormone treatment. Toxicology Letters, 119, 87-93. doi:10.1016/S0378-4274(00)00295-2
[26] Bagchi, D., Stohs, S.J., Downs, B.W., Bagchi, M. and Preuss, H.G. (2002) Cytotoxicity and oxidative mechanisms of different forms of chromium. Toxicology, 180, 5-22. doi:10.1016/S0300-483X(02)00378-5
[27] Cuypers, A., Plusquin, M., Remans, T., Jozefczak, M., Keunen, E., Gielen, H., Opdenakker, K., Nair, A.R., Munters, E., Artois, T.J., Nawrot, T., Vangronsveld, J. and Smeets, K. (2010) Cadmium stress: An oxidative challenge. Biometals, 23, 927-940. doi:10.1007/s10534-010-9329-x
[28] Ercal, N., Gurer-Orhan, H. and Aykin-Burns, N. (2001) Toxic metals and oxidative stress. Part 1. Mechanisms involved in metal-induced oxidative damage. Current Topics in Medicinal Chemistry, 1, 529-539. doi:10.2174/1568026013394831
[29] Gackowski, D., Kruszewski, M., Jawien, A., Ciecierski, M. and Olinski, R. (2001) Further evidence that oxidative stress may be a risk factor responsible for the development of atherosclerosis. Free Radical Biology and Medicine, 31, 542-547. doi:10.1016/S0891-5849(01)00614-1
[30] Fraga, C.G. and Oteiza, P.I. (2002) Iron toxicity and anti-oxidant nutrients. Toxicology, 180, 23-32. doi:10.1016/S0300-483X(02)00379-7
[31] Bush, A.I. (2008) Drug development based on the metals hypothesis of Alzheimer’s disease. Journal of Alzheimer′s Disease. 15, 223-240.
[32] Durackova, Z. (2010) Some current insights into oxidative stress. Physiological Research, 59, 459-469.
[33] O’Brien, T., Mandel, H.G., Pritchard, D.E. and Patierno, S.R. (2002) Critical role of chromium (Cr)-DNA interactions in the formation of Cr-induced polymerase arresting lesions. Biochemistry, 41, 12529-12537. doi:10.1021/bi020452j
[34] Quievryn, G., Peterson, E., Messer, J. and Zhitkovich, A. (2003) Genotoxicity and mutagenicity of chromium (VI)/ ascorbate-generated DNA adducts in human and bacterial cells. Biochemistry, 42, 1062-1070. doi:10.1021/bi0271547
[35] Waisberg, M., Joseph, P., Hale, B. and Beyersmann, D. (2003) Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology, 192, 95-117. doi:10.1016/S0300-483X(03)00305-6
[36] Price, D.J. and Joshi, J.G. (1983) Ferritin. Binding of beryllium and other divalent metal ions. Journal of Biological Chemistry, 258, 10873-10880.
[37] Watjen, W. and Beyersmann, D. (2004) Cadmium-induced apoptosis in C6 glioma cells: Influence of oxidative stress. Biometals, 17, 65-78. doi:10.1023/A:1024405119018
[38] Gurer, H. and Ercal, N. (2000) Can antioxidants be beneficial in the treatment of lead poisoning? Free Radical Biology and Medicine, 29, 927-945. doi:10.1016/S0891-5849(00)00413-5
[39] Garcon, G., Garry, S., Gosset, P., Zerimech, F., Martin, A., Hannothiaux, M.H. and Shirali, P. (2001) Benzo(a)-pyrene-coated onto Fe2O3 particles induced lung tissue injury: role of free radicals. Cancer Letters, 167, 7-15. doi:10.1016/S0304-3835(01)00474-8
[40] Garcon, G., Ledoux, F., Hannothiaux, M.H., Zerimech, F., Puskaric, E. and Shirali, P. (2002) Urban particulate air pollution and evaluation of its toxicity on human pulmonary cells in culture. 6th International Aerosol Conference Taiwan.
[41] Tilman, G. (2005) Oxidants and antioxidants defense systems. In: The handbook of environmental chemistry 2.0. S pringer Berlin Heidelberg, New York.
[42] Bonnefont-Rousselot, D., Therond, P., Beaudeux, J.L., Peynet, J., Legrand, A. and Delatrade, J. (2001) Aging and oxidative stress. Which potential markers? Annales de Biologie Clinique, 59, 453-459.
[43] Jomova, K. and Valko, M. (2011) Advances in metal-induced oxidative stress and human disease. Toxicology, 283, 65-87. doi:10.1016/j.tox.2011.03.001

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