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The Fate of Aflatoxin in Corn Fermentation

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DOI: 10.4236/nr.2012.33017    4,826 Downloads   8,518 Views   Citations


A lab-scale ethanol fermentation was investigated to determine where aflatoxin concentrated during each phase of production. Four corn samples with high levels of aflatoxin (ranging from 7750 – 17,208 parts per billion) and their replicates were compared with a replicated negative control. Fractions were taken from the fermented mash, distilled ethanol, stillage, and dried corn solids (DCS). These fractions were analyzed using two different immunoassay methods and liquid chromatography tandem mass spectrometry (LC-MS/MS). Results indicated no aflatoxin was found in the distilled ethanol. Some aflatoxin (13%) was detected in the stillage, but most of the toxin was recovered in the DCSs ranging from 31% to 58%. A second series of experiments were conducted to investigate the effect of binders on dried distillers grains (DDGs). A brewers dried yeast anti-caking binder that contains glucomannon (MTB-100?), was mixed with contaminated DDGs. Addition of the binder showed a significant reduction in aflatoxin levels in comparison to a positive control. Aflatoxin binding at 2% binder w/w reached 72.5% and showed a minimal binding percentage increase of 80% at 6% binder w/w. Testing was also conducted to determine if environmental variables such as pH and temperature had any effect on the binding capabilities. Temperature near 0?C resulted in binding at 19.7% at a pH range of 6 to 8. Additionally, at a temperature of 40?C resulted in binding of 36%, 47%, and 45% at pHs 6, 7, and 8, respectively. These findings suggest that the addition of sorbents may be an effective way of salvaging contaminated DDGs.

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C. Johnston, R. Singleterry, C. Reid, D. Sparks, A. Brown, B. Baldwin, S. Ward and W. Williams, "The Fate of Aflatoxin in Corn Fermentation," Natural Resources, Vol. 3 No. 3, 2012, pp. 126-136. doi: 10.4236/nr.2012.33017.


[1] J. W. Bennett and M. Klich, “Mycotoxins,” Clinical Microbiology Revies, Vol. 16, No. 3, 2003, pp. 497-516. doi:10.1128/CMR.16.3.497-516.2003
[2] G. A. Payne, “Epidemiology of Aflatoxin Formation by A. flavus. Aflatoxin and Aspergillus flavus in Corn,” Southern Cooperative Series Bulletin, Vol. 279, 1983, pp. 16-19.
[3] M. A. Klich, L. S. Lee and H. E. Huizar, “The Occurrence of Aspergillus flavus in vegetative tissue of Cotton Plants And Its Relation to Seed Infection,” Mycopathologia, Vol. 95, No. 3, 1986, pp. 171-174. doi:10.1007/BF00437123
[4] H. S. Lillard, R. T. Hanlin and D. A. Lillard, “Aflatoxigenic Isolates of Aspergillus flavus from Pecans,” Applied Microbiology, Vol. 19, No. 1, 1970, pp. 128-130.
[5] W. D. Price, R. A. Lovell and D. G. McChesney, “Naturally Occurring Toxins in Feedstuffs: Center for Veterinary Medicine Perspective,” Journal of Animal Science, Vol. 71, No. 9, 1993, pp. 2556-2562.
[6] H. M. Martins, M. L. Martins and F. M. A. Bernardo, “Bacillaceae Spores, Fungi and Aflatoxin Determination in Honey,” RPCV (Revista Portuguesa De Ciências Vet- erinárias), Vol. 98, No. 546, 2003, pp. 85-88.
[7] C. Bircan, S. A. Barringer, U. Ulken and R. Pehlivan, “Increased Aflatoxin Contamination of Dried Figs in a Drought Year,” food Additives and Contaminants, Part A, Vol. 25, No. 11, 2008, pp. 1400-1408.
[8] N. Delage, A. d’Harlingue, B. C. Ceccaldi and G. Bompeix, “Occurrence of Mycotoxins in Fruit Juices and Wine,” Food Control, Vol. 14, No. 4, 2008, pp. 225-227. doi:10.1016/S0956-7135(03)00010-0
[9] P. Rahimi, B. Sharifnabi and M. Bahar, “Detection of Aflatoxin in Aspergillus Species Isolated from Pistachio in Iran,” Journal of Phytopathology, Vol. 156, No. 1, 2008, pp. 15-20. doi:10.1111/j.1439-0434.2007.01312.x
[10] Council for Agricultural Science and Technology, “My- cotoxins, Economics and Health Risks,” Report No. 116, Ames, IA. 1980.
[11] Council for Agricultural Science and Technology (CAST) “Aflatoxins and Other Mycotoxins, an Agricultural Perspective,” Report No. 80. Ames, IA. 1979.
[12] International Agency for Research on Cancer, “Some Traditional Herbal Medicines, Some Mycotoxins, Napthalene and Styrene,” Vol. 82, IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, World Health Organization, Geneva, 2002, pp. 171-175.
[13] W. P. Blount, “Turkey ‘X’ Disease,” Turkeys, Vol. 9, No. 7, 1961, pp. 52-61.
[14] D. L. Wei and S. C. Jong, “Production of Aflatoxins by Strains of the Aspergillus flavus Group Maintained at ATCC,” Mycopathologia, Vol. 93, No. 1, 1986, pp. 19-24. doi:10.1007/BF00437010
[15] M. A. Klich and J. I. Pitt, “Differentiation of Aspergillus flavus from A. parasiticus and Other Closely Related Species,” Fragments of training set structures, Vol. 91, No. 1, 1988, pp. 99-108. doi:10.1016/S0007-1536(88)80010-X
[16] R. A. Squire, “Rating Animal Carcinogens: A Proposed Regulatory Approach,” Science, Vol. 214, No. 4523, 1981, pp. 877-880. doi:10.1126/science.7302565
[17] J. M. Cullen and P. M. Newberne, “Acute Hepatotoxicity of Aflatoxins,” In: D. L. Eaton and J. D Groopman, Eds., The Toxicology of Aflatoxins: Human Health, Veterinary, and Agricultural Significance, Academic Press, San Diego, 1994, pp. 3-26.
[18] H. P. van Egmond, “Aflatoxin M1: Occurrence, Toxicity, Regulation,” In: H. P. van Egmond, Ed., Mycotoxins in Dairy Products, Elsevier Applied Science, London, 1989, pp. 11-55.
[19] S. C. Pei, Y. Y. Zhang, S. A. Eremin and W. J. Lee, “Detection of Aflatoxin M1 in Milk Products from China by ELISA Using Monoclonal Antibodies,” Food Control, Vol. 20, No. 12, 2009, pp. 1080-1085. doi:10.1016/j.foodcont.2009.02.004
[20] M. A. Klich, “Biogeography of Aspergillus Species in Soil and Litter,” Mycologia, Vol. 94, No. 1, 2002, pp. 21-27. doi:10.2307/3761842
[21] M. A. Klich, “Aspergillus flavus: The Major Producer of Aflatoxin,” Molecular Plant Pathology, Vol. 8, No. 6, 2007, pp. 713-722. doi:10.1111/j.1364-3703.2007.00436.x
[22] H. P. van Edmond and M. A. Jonker, “Worldwide Regulations on Aflatoxins,” In: H. K. Abbas, Ed., Aflatoxin and Food Safety, Taylor and Francis, Boca Raton, 2005, pp. 77-93.
[23] “US Federal Drug Agency (FDA), Office of Regulatory Affairs (ORA) Compliance Policy Guides (CPG) 7126.33 Sec. 683.100—Action Levels for Aflatoxin in Animal Feeds, Issued Nov 21, 1979, reissued Oct 1, 1980, revised Aug 15, 1982, May 18, 1989, and Aug 28,” 1994.
[24] S. D. Donner and C. J. Kucharik, “Corn-Based Ethanol Production Comprises Goal of Reducing Nitrogen Export by the Mississippi River,” Proceedings of the National Academy of Sciences, Vol. 18, No. 105, 2008, pp. 4513-4518. doi:10.1073/pnas.0708300105
[25] S. A. Watson, “Structure and Composition” In: S. A. Watson and P. E. Ramstad, Eds., Corn: Chemistry and Technology, American Association of Cereal Chemists, Inc., St. Paul, 1987 pp. 53-82.
[26] F. Wu and G. P. Munkvold, “Mycotoxins in Ethanol Co-Products: Modeling Economic Impacts on the Livestock Industry and Management Strategies,” Journal of Agricultural and Food Chemistry, Vol. 56, No. 11, 2008, pp. 3900-3911. doi:10.1021/jf072697e
[27] M. J. Spiehs, M. H. Whitney and G. C. Shurson. “Nutrient Database for Distiller’S Dried Grains with Solubles Produced from New Ethanol Plants in Minnesota and South Dakota,” Journal of Animal Science, Vol. 80, No. 10, 2002, pp. 2639
[28] D. J. Schingoethe, “Corn Co Products for Cattle,” Pro- ceedings from 40th Eastern Nutrition Conference, Ottawa, 11-12 May 2004, pp. 30-47.
[29] B. S. Dien, R. B. Hespell, L. O. Ingram and R. J. Bothast, “Conversion of Corn Milling Fibrous Co-Products into Ethanol by Recombinant Escherichia Coli Strains K011 and SL40,” World Journal of Microbiology and Biotechnology, Vol. 13, No. 6, 1997, pp. 619-625. doi:10.1023/A:1018554417934
[30] B. C. Saha, B. S. Dien and R. J. Bothast, “Fuel Ethanol Production from Corn Fiber, Current Status and Technical Prospects,” Applied Biochemistry and Biotechnology, Vol. 70-72, No. 1, 1998, pp. 115-125. doi:10.1007/BF02920129
[31] Y. Kim, N. S. Mosier, R. Hendrickson, T. Ezeji, H. Blaschek, B. Dien, M. Cotta, B. Dale and M. R. Ladisch, “Composition of Corn Dry-Grind Ethanol By-Products: DDGS, Wet Cake, and Thin Stillage,” Bioresource Technology, Vol. 99, No. 12, 2008, pp. 5165-5176. doi:10.1016/j.biortech.2007.09.028
[32] F. S. Chu, C. C. Shang, S. H. Ashoor and N. Prentice, “Stability of Aflatoxin B1 and Ochratoxin A in Brewing,” Journal of Applied Microbiology, Vol. 29, No. 3, 1975, pp. 313-316.
[33] R. Dam, S. W. Tam and L. D. Satterlee, “Destruction of Aflatoxins during Fermentation and By-Product Isolation from Artificially Contaminated Grains,” Cereal Chemistry, Vol. 54, No. 3, 1977, pp. 705-714.
[34] E. B. Lillehoj, W. F. Maisch and A. Lagoda, “The Fate of Aflatoxin in Naturally Contaminated Corn during the Ethanol Fermentation,” Canadian Journal of Microbiology, Vol. 25, No. 8, 1979, pp. 911-914. doi:10.1139/m79-135
[35] R. J. Bothast, G. A. Bennett, J. E. Vancauwenberge and J. L. Richard, “Fate of Fumonisin B1 in Naturally Contaminated Corn during Ethanol Fermentation,” Applied and Environmental Microbiology, Vol. 58, No. 1, 1992, pp. 233-236.
[36] Z. S. C. Okoye, “Carryover of Aflatoxin B1 in Contaminated Substrate Corn in Nigerian Native Beer,” Bulletin of Environmental Contamination and Toxicology, Vol. 37, No. 1, 1986, pp. 482-489. doi:10.1007/BF01607792
[37] L. L. Southern and A. J. Clawson, “Ammoniation of Corn Contaminated with Aflatoxin and Its Effects on Growing Rats,” Journal of Animal Science, Vol. 50, No. 3, 1982, pp. 459-466.
[38] M. D. Buser and H. K Abbas, “Effects of Extrusion Temperature and Dwell Time on Aflatoxin Levels in Cottonseed,” Journal of Agricultural and Food Chemistry, Vol. 50, No. 9, 2002, pp. 2556-2559. doi:10.1021/jf011479x
[39] J. A. Méndez-Albores, G. A. Villa, J. C. Del Rio-García and E. M. Martínez, “Aflatoxin-Detoxification Achieved with Mexican Traditional Nixtamalization Process (MTNP) Is Reversible,” Journal of the Science of Food and Agriculture, Vol. 84, No. 12, 2004, pp. 1611-1614. doi:10.1002/jsfa.1853
[40] I. Ghanem, M. Orfi and M. Shamma, “Effect of Gamma Radiation on the Inactivation of Aflatoxin B1 in Food and Feed Crops,” Brazilian Journal of Microbiology, Vol. 39, No. 4, 2008, pp. 787-791. doi:10.1590/S1517-83822008000400035
[41] S. Herzallah, K. Alshawabkeh and A. Al Fataftah, “Aflatoxin Decontamination of Artificially Contaminated Feeds by Sunlight, γ-Radiation, and Microwave Heating,” The Journal of Applied Poultry Research, Vol. 17, No. 4, 2008, pp. 515-521. doi:10.3382/japr.2007-00107
[42] The Code of Federal Regulations, “Title 21, Food and Drugs; Part 582, Substances Generally Recognized as Safe; Subpart c, Anticaking Agents; Section 582.2727, Sodium Aluminosilicate,” Federal Register, Vol. 77, 2011, p. 567.
[43] The Code of Federal Regulations, “Title 21, Food and Drugs; Part 582, Substances Generally Recognized as Safe; Subpart c, Anticaking Agents; Section 582.2729, Hydrated Sodium Calcium Aluminosilicate,” Federal Register, Vol. 77, 2011, p. 568.
[44] D. E. Diaz, W. M. Hagler Jr., B. A. Hopkins and L. W. Whitlow, “Aflatoxin binders I: In Vitro Binding Assay for Aflatoxin B1 by Several Potential Sequestering Agents,” Mycopathologia, Vol. 156, No. 3, 2002, pp. 223-226. doi:10.1023/A:1023388321713
[45] J. C. R. Demythenaere, C. Dagher, P. Sandra, S. Kallithraka, R. Verhe and N. De Kimpe, “Flavour Analysis of Greek White Wine by Solid-Phase Microextraction-Capillary Gas Chromatography,” Journal of Chro- matography A, Vol. 985, No. 1-2, 2003, pp.127-153.
[46] G. Piva, F. Galvano, A. Pietri and A. Piva, “Detoxification Methods of Aflatoxins: A Review,” Nutrition Research, Vol. 15, No. 5, 1995, pp. 767-776. doi:10.1016/0271-5317(95)00042-H
[47] M. Peraica, A. M. Domijan, ?. Jurjevi? and B. Cvjetkovi?, “Prevention of Exposure to Mycotoxins From Food and Feed,” Archives of Industrial Hygiene and Toxicology, Vol. 53, No. 3, 2002, pp. 229-237.
[48] D. E. Diaz, W. M. Hagler Jr., J. T. Blackwelder, J. A. Eve, B. A. Hopkins, K. L. Anderson, F. T. Jones and L. W. Whitlow, “Aflatoxin Binders II: Reduction of Aflatoxin M1 in Milk by Sequestering Agents of Cows Consuming Aflatoxin In Feed,” Mycopathologia, Vol. 157, No. 2, 2004, pp. 1-8.
[49] M. G. Tenorio Arvide, I. Mulder, A. L. B. Velazquez and J. B. Dixon, “Smectite Clay Adsorption of Aflatoxin vs. Octahedral Composition as Indicated by FTIR,” Clays and Clay Minerals, Vol. 56, No. 5, 2008, pp. 571-578. doi:10.1346/CCMN.2008.0560510
[50] I. A. Samdal, J. A. B. Aasen, L. R. Briggs, E. Dahl and C. O. Miles, “Comparison of ELISA and LC-MS Analyses for Yessotoxins in Blue Mussels (Mytilus edulis),” Toxicon, Vol. 46, No. 1, 2005, pp. 7-15. doi:10.1016/j.toxicon.2005.02.003
[51] Z. Zheng, J. Hanneken, D. Houchins, R. S. King, P. Lee and J. L. Richard, “Validation of an ELISA Test Kit for the Detection of Ochratoxin A in Several Food Commodities by Comparison with HPLC,” Mycopathologia, Vol. 159, No. 2, 2005, pp. 265. doi:10.1007/s11046-004-8663-3
[52] H. Colak, H. Hampikyan, B. Ulusoy and O. Ergun, “Comparison of Competitive ELISA with an HPLC Method for the Determination of Aflatoxin M1 in Turkish White, Kasar and Tulum cheeses,” European Food Research and Technology, Vol. 223, No. 6, 2006, pp. 719- 723. doi:10.1007/s00217-006-0258-4
[53] J. M. Faupel-Badger, B. J. Fuhrman, X. Xu, R. T. Falk, L. K. Keefer, T. D. Veenstra, R. N. Hoover and R. G. Ziegler, “Comparison of Liquid Chromatography-Tandem Mass Spectrometry, RIA, and ELISA Methods for Measurement of Urinary Estrogens,” Cancer Epidemiology, Biomarkers & Prevention, Vol. 19, No. 1, 2010 pp. 292-300. doi:10.1158/1055-9965.EPI-09-0643
[54] US Food and Drug Administration, Center for Veterinary Medicine, “Nationwide Survey of Distillers Grains for Aflatoxins,” 2006.
[55] G. S. Murthy, D. E. Townsend, G. L. Meerdink, G. L. Bargren, M. E. Tumbleson and V. Singh, “Effect of Aflatoxin B1 on the Dry-Grid Ethanol Process,” Cereal Chemistry, Vol. 82, No. 3, 2005, pp. 302-304. doi:10.1094/CC-82-0302
[56] G. A. Bennett, A. A. Lagoda, O. L. Shotwell and C. W. Hesseltine, “Utilization of Zearalenone-Contaminated Corn for Ethanol Production,” Journal of the American Oil Chemists’ Society, Vol. 58, No. 11, 1981, pp. 974-976. doi:10.1007/BF02659774
[57] S. Fruhauf, H. Schwartz, F. Ottner, R. Krska and E. Vekiru, “Yeast Cell Based Feed Additives: Studies on Aflatoxin B1 and Zearalenone,” Food additives & Contaminants, Vol. 29, No. 2, 2012, pp. 217-231. doi:10.1080/19440049.2011.630679

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