Antihepatotoxic Effect of Feronia Limonia Fruit against Carbon Tetrachloride Induced Hepatic Damage in Albino Rats

Rucha Upadhyay, Narendra Deo Pandey, Shahid Suhail Narvi, Amita Verma, Bahar Ahmed

.

DOI: 10.4236/cm.2010.11003                

Abstract

Feronia limonia Swingle, though mentioned as a high utility medicinal plant in Ayurveda for the treat-ment of liver disorders, but has no scientific investigations. Therefore, the present study was undertaken to investigate the hepatoprotective activity of extracts of Feronia limonia fruit on albino rat liver, damage induced by single dose of carbon-tetrachloride (1.5 ml/kg, p.o.). The oral administration of extracts (500 mg/kg, p.o.) for 7 days has shown significant hepatoprotective action. There is reduction in the elevated levels of serum enzymes such SGOT, SGPT, ALP and Serum bilirubin and elevation in level of GSH, GPX,GLY and total protein in Feronia limonia protected rats.

Keywords

Anti-Hepatotoxic, Carbon Tetrachloride, Feronia Limonia Swingle

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	J. D. Hooker, “The Flora of British India,” L. Reeve & Co, London, 1875, p. 178.
[2]	K. R. Kirtikar, B. D. Basu and I. C. S. An, “Indian Medicinal Plants,” Orient Enterprises, Dehradun, India, 1993, Vol. 1, pp. 496-498.
[3]	M.Yusuf, J. U. Chowdhury, M. A. Wahab and J. Begum, “Medicinal Plants of Bangladesh,” BCSIR Laboratories. Chittagong, 1994, pp. 112-114.
[4]	L. M. Perry, “Medicinal plants of East and Southern Asia,” The MIT Press, Cambridge, 1980, pp. 87-88.
[5]	S. K. Talapatra, M. K. Chaudhuri and B. Talapatra, “Coumarins from the Root Bark of Feronia Elephantum,” Phytochemistry, Vol. 12, 1973, pp. 236-237.
[6]	S. R. Gupta, T. R. Seshadri, C. S. Sharma and N. D. Sharma, “Chemical Components of Feronia limonia,” Planta Medica, Vol. 36, 1979, pp. 95-96.
[7]	A. Agrawal, I. R. Siddique and J. Singh, “Coumarins from the Roots of Feronia limonia,” Phytochemistry, Vol. 28, 1989, pp. 1229-1232.
[8]	A. J. Macleod and N. M. Pieris, “Volatile Flavour Components of Wood Apple (Feronia limonia) and a Processed Product,” Journal of Agricultural and Food Chemistry, Vol. 29, 1981, pp. 49-53.
[9]	A. Ahmad, L. N. Misra and R. S. Thakur, “Composition of the Volatile Oil from Feronia Limonia Leaves,” Planta Medica, Vol. 55, 1989, pp. 199-200.
[10]	B. Ahmed, T. A. Al-Howiriny and A. B. Siddique, Journal of Ethanopharmacology, Vol. 7, 2003, p. 237.
[11]	S. Reitman and S. Frankel, “In Vitro Determination of Transaminase Activity in Serum,” American Journal of Clinical Pathology, Vol. 28, 1957, p. 56.
[12]	P. R. N. Kind and E. J. King, “Estimation of Plasma Phosphatase by Determination of Hydrolysed Phenol with Amino Antipyrine,” Journal of Clinical Pathology, Vol. 7, 1954, p. 322.
[13]	O. H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall, “Protein Measurement with Folin-Phenol Reagent,” Journal of Biological Chemistry, Vol. 193, 1951, p. 265.
[14]	L. Jendrassik and P. Grof, “Vereinfachte Photo-Metrische Methoden zur Bestimmung des Bilirubins,” Biochemische Zeitschrift, Vol. 297, 1938, pp. 81-89.
[15]	E. Beutler, O. Duran and B. M. Kelly, “Improved Method for the Determination of Blood Glutathione,” Journal of Laboratory Clinical Medicine, Vol. 61, 1963, p. 882.
[16]	W. Z. Hassid and S. Abraham, “Chemical Procedures for Analysis of Polysaccarides,” In: S. P. Colowick and N. O. Kaplan Eds., Methods in Enzymology, Academic Press Inc., New York, Vol. 3, 1957, pp. 34.
[17]	J. T. Rotruck, A. L. Pope, H. E. Ganther, D. G. Hafeman and W. G. Hoekstra, “Selenium: biochemical role as a component of glutathione peroxidase,” Science, Vol. 179, 1973, pp. 588-590.
[18]	A. E. Galigher and E. N. Kozloff, Essentials of Practical Microtechnique, 2nd Edition, Philadelphia, 1971, pp. 210.
[19]	K. H. Cheeseman, E. F. Albano, A. Thomasi and T. Slater, “Biochemical Studies on the Metabolic Activation of Halogenated Alkanes,” Environmental Health Perspectives, Vol. 64, 1985, p. 85.
[20]	K. Singh, A. K. Khanna and R. Chandan, “Hepatoprotective Activity of Ellagic Acid against Carbon Tetrachloride Induced Hepatotoxicity in Rats,” Indian Journal of Experimental Biology, Vol. 37, 1999, pp. 1025-1026.