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The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in Arabidopsis thaliana

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DOI: 10.4236/ajmb.2012.23025    3,591 Downloads   6,208 Views   Citations

ABSTRACT

We have previously described a simple method to analyze the chromatin structure of Arabidopsis telomeres independently of that of Interstitial Telomeric Sequences (ITSs). By using this method, we found that, whereas ITSs are heterochromatic, Arabidopsis telomeres exhibit euchromatic features [1]. Some concerns have been recently raised about the accuracy of this procedure [2]. Here, we summarize these concerns and justify our experimental approaches and interpretation of results.

Cite this paper

Vaquero-Sedas, M. and Vega-Palas, M. (2012) The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in Arabidopsis thaliana. American Journal of Molecular Biology, 2, 242-244. doi: 10.4236/ajmb.2012.23025.

References

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[2] Majerová, E., Fojtová, M., Mandáková, T. and Fajkus, J. (2011) Methylation of plant telomeric DNA: what do the results say? Plant Molecular Biology, 77, 533-536. doi:10.1007/s11103-011-9834-5
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[4] Gámez-Arjona, F., López-López, C., Vaquero-Sedas, M. and Vega-Palas, M. (2010) On the organization of the nucleosomes associated with telomeric sequences. Biochimica et Biophysica Acta, 1803, 1058-61. doi:10.1016/j.bbamcr.2010.03.021
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[6] Uchida, W., Matsunaga, S., Sugiyama, R. and Kawano, S. (2002) Interstitial telomere-like repeats in the Arabidopsis thaliana genome. Genes Genetic Systems, 77, 63-7. doi:10.1266/ggs.77.63
[7] Vannier, J., Depeiges, A., White, C. and Gallego, M. (2009) ERCC1/XPF protects short telomeres from homologous recombination in Arabidopsis thaliana. PLoS Genetics, 5, e1000380. doi:10.1371/journal.pgen.1000380
[8] Pisano, S., Galati, A. and Cacchione, S. (2008) Telomeric nucleosomes: Forgotten players at chromosome ends. Cell Molecular Life Science. doi:10.1007
[9] Lippman, Z., Gendrel, A., Colot, V. and Martienssen, R. (2005) Profiling DNA methylation patterns using genomic tiling microarrays. Nat Methods, 2, 219-24. doi:10.1038/nmeth0305-219

  
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