BOD Exertion and OD600 Measurements in Presence of Heavy Metal Ions Using Microbes from Dairy Wastewater as a Seed

Susheel K. Mittal, Siloni Goel

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DOI: 10.4236/jwarp.2010.25055                

Abstract

BOD measurements in presence of cobalt, nickel, copper, zinc, silver and cadmium are reported using wastewater from dairy industry as a seed. Extent of inhibition in BOD is studied for variables like, concen-tration of metal ions (0.2 mM to 1.4 mM), pH (3 to 8) and temperature of incubation (15℃, 20℃, 25℃, 30℃ and 35℃). Results of BOD inhibition are supported by absorbance measurement (OD600) studies of microbial matter preserved in Luriya broth medium. OD measurement results are used to derive minimum inhibitory concentration, i.e., threshold concentration of each metal showing toxicity towards microbes. Sil-ver is found to be the most toxic element.

Keywords

BOD Inhibition, Metal Ions, OD600, Microbes, Dairy Wastewater

Conflicts of Interest

The authors declare no conflicts of interest.

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