Effect of intraperitoneal and intramuscular injection of killed aeromonas hydrophila on lymphocytes and serum proteins of common carp, cyprinus carpio

Rahim Peyghan, Gholamhosain H. Khadjeh, Naghmeh Mozarmnia, Maryam Dadar

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DOI: 10.4236/abb.2010.11004                

Abstract

The effects of injectable killed Aeromonas hydrophila on lymphocyte populations and on serum proteins of juvenile common carp, Cyprinus carpio, were examined. The fishes were injected either intraperitoneally or intramuscularly with killed A. hydrophila bacterin isolated from a diseased fish. 15 days after injection the lymphocyte count was significantly higher in the kidney of intraperitoneally bacteria injected fishes than in the normal saline injected fishes (p < 0.05), but in the blood smears the lymphocyte count difference was not significant (p > 0.05). In the intramuscular bacteria injected fishes in comparison with the normal saline group, the lymphocyte count was significantly higher only in the blood (p < 0.05) but in the kidney the difference was not significant (p > 0.05). In comparison between the serum protein parameters in different groups, in intraperitoneal injection of killed-bacteria, total albumin as well as the ratio of albumin/globulin was greater than the control group (p < 0.05). The other fractions had not showed any significant difference (p > 0.05). In intramuscular injection, the normal saline-injected group, amount of α?-globulin was significantly higher than bacteria-injected group (p < 0.05). For the other fractions there was no significant difference between two groups (p > 0.05).

Keywords

Aeromonas hydrophila; Globulins; Albumin, Lymphocytes; Vaccination

Conflicts of Interest

The authors declare no conflicts of interest.

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