Author(s)

Asmaa Elframawy¹, Hisham Deif², Ranya El-Bakatoushi^3*

¹Nucleic Acids Research Department, Genetic Engineering & Biotechnology Research Institute (GEBRI), City for Scientific Research, Borg Elarab, Alexandria, Egypt.
²Botany Department, Faculty of Science, University of Alexandria, Alexandria, Egypt.
³Biology and Geology Sciences Department, Faculty of Education, University of Alexandria, Alexandria, Egypt.

Two forms of A. halimus shrubs: erect habit (A. halimus) and bushy habit shrub (A. schweinfurthii) are used naturally isolated by a considerable distance from each other and occupy the same area. To explore the effect of natural isolation on the genetic basis of the two forms, Start Codon Targeted (SCoT) and the phylogenetic relationships of A. halimus by sequencing ITS1-5.8S-ITS2 regions of the ribosomal DNA are used. Significant isolation-by-distance relationship was found (r = 0.62, P = 0.001). Soil factors did not influence molecular variations. The natural isolation of A. halimus habitats restricts gene flow among the populations and the observed high within-population genetic diversity (74.19%) in this species is best explained by its outcrossing behaviour, long-lived individuals and overlapping generations. The UPGMA analysis of the SCoT results showed that all the studied populations were divided into two discrete genetic groups with significant separation of the two forms in Burg El-Arab area (Populations 1 and 2) and insignificant separation between two forms in El-Hammam area (population 5 and 6). The sequencing of the ITS1-5.8S-ITS2 rDNA regions also showed the insignificant separation of the two A. halimus forms. We conclude that gene flow depending on habitat fragmentation was the main factor affecting the population genetic differentiation. We suggest that the two forms do not merit specific rank in presence of interference between the two forms and absence of a breeding barrier fail to separate the different populations when they become sympatric.

Atriplex halimus, SCoT, ITS1-5.8S-ITS2 rDNA Regions, Atriplex schweinfurthii

Elframawy, A. , Deif, H. and El-Bakatoushi, R. (2016) Genetic Variation among Fragmented Populations of Atriplex halimus L. Using Start Codon Targeted (SCoT) and ITS1-5.8S-ITS2 Region Markers. American Journal of Molecular Biology, 6, 101-115. doi: 10.4236/ajmb.2016.62011.