A sero-prevalence survey of Toxoplasmosis in domestic animals (camels, cattle, sheep and goats) was conducted in the Benadir region of Somalia from January to July 2014 to determine the status of the disease. A total of 151 sera were tested, 64 camels; 28 cattle; 29 sheep and 30 goats, for the presence of Toxoplasma gondii antibodies using Latex Agglutination Test (LAT). Totally about 15.9% (24/151) of studied animals showed sero-positive. The sero-prevalence was found in 6.3% of 64 camels, 7.1% of 28 cattle, 34.5% of 29 sheep and 26.7% of 30 goats. The antibody titres to T. gondii positive sera were 2 (8.3%), 9 (37.5%), 4 (16.7%), 4 (16.7%), 4 (16.7%), 1 (4.2%) and 0 (0%) by dilution of 1:2, 1:4, 1:8, 1:16, 1:32, 1:64 and 1:128 respectively. Out of 151 domestic animals (45 male and 106 female), 8 (5.3%) males and 16 (10.6%) females were infected with T. gondii. The study recommends the need for further researches in the whole country using different serological tests and to determine the impact of these findings on the human population. It would be important to increase public awareness on zoonotic potential of Toxoplasmosis.