Author(s)

Tanzila Shahzadi, Zahid Anwar, Zafar Iqbal, Awais Anjum, Tahir Aqil, Bakhtawar  , Arroha Afzal, Muhammad Kamran, Sajid Mehmood, Muhammad Irshad

Agriculture Biotechnology, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Department of Biochemistry, Nawaz Sharif Medical College, University of Gujrat, Gujrat, Pakistan.
Department of Botany, University of Gujrat, Gujrat, Pakistan.
Department of Microbiology, Quaid-e-Azam University, Islamabad, Pakistan.

In the present study, a co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase using cheap and easily available agro-industrial residue corn stover as growth supporting substrate. Various physio-chemical and nutritional variables were optimized using classical and completely randomized designs for induced production of exoglucanase, and β-glucosidase from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state fermentation (SSF). Analysis profile showed that when the conditions of the SSF medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with 6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ± 6.5 U/mL) and β-glucosidase (255 ± 3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35°C.

Process Optimization; SSF; Co-Culture; T. viride; G. lucidum

Shahzadi, T. , Anwar, Z. , Iqbal, Z. , Anjum, A. , Aqil, T. ,  , B. , Afzal, A. , Kamran, M. , Mehmood, S. and Irshad, M. (2014) Induced Production of Exoglucanase, and β-Glucosidase from Fungal Co-Culture of T. viride and G. lucidum. Advances in Bioscience and Biotechnology, 5, 426-433. doi: 10.4236/abb.2014.55051.